Results 1 to 10 of about 114,219 (358)
dsRNA-Seq: Identification of viral infection by purifying and sequencing dsRNA [PDF]
Viruses, 2019 AbstractRNA viruses are a major source of emerging and re-emerging infectious diseases around the world. We developed a method to identify RNA viruses that is based on the fact that all RNA viruses produce dsRNA while replicating. Purifying and sequencing dsRNA from total RNA isolated from infected tissue allowed us to recover replicating viral ...
Alex C. Stabell +12 more
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Mathematical modelling of OAS2 activation by dsRNA and effects of dsRNA lengths
AIMS Mathematics, 2021 The activation of 2'-5'-oligoadenylate synthetase (OAS) enzymes by direct interaction with viral double-stranded RNA (dsRNA) is a key part of the innate immune response to viral infection. A downstream effect of the OAS-dsRNA interaction is to degrade the single-stranded RNA to prevent the spread of the virus. The activation of OAS2, one of the members
Nikhat Lubna +4 more
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Barriers to Efficient Foliar Uptake of dsRNA and Molecular Barriers to dsRNA Activity in Plant Cells [PDF]
Frontiers in Plant Science, 2020 Foliar application of dsRNA to elicit an RNA interference (RNAi) response is currently under consideration as a crop protection strategy. To access the RNAi machinery of a plant, foliarly applied dsRNAs must traverse the plant cuticle, avoid nuclease degradation, and penetrate the cell wall and plasma membrane.
Bill Hendrix +3 more
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Cold Spring Harbor Perspectives in Biology, 2019 Long double-stranded RNAs (dsRNAs) are abundantly expressed in animals, in which they frequently occur in introns and 3' untranslated regions of mRNAs. Functions of long, cellular dsRNAs are poorly understood, although deficiencies in adenosine deaminases that act on RNA, or ADARs, promote their recognition as viral dsRNA and an aberrant immune ...
Daniel P. Reich, Brenda L. Bass
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Double-Stranded RNA Attenuates the Barrier Function of Human Pulmonary Artery Endothelial Cells [PDF]
, 2013 Circulating RNA may result from excessive cell damage or acute viral infection and can interact with vascular endothelial cells. Despite the obvious clinical implications associated with the presence of circulating RNA, its pathological effects on ...
A Shibamiya +60 more
core +14 more sources
SID-1 is a dsRNA-selective dsRNA-gated channel [PDF]
RNA, 2011 Systemic RNAi in Caenorhabditis elegans requires the widely conserved transmembrane protein SID-1 to transport RNAi silencing signals between cells. When expressed in Drosophila S2 cells, C. elegans SID-1 enables passive dsRNA uptake from the culture medium, suggesting that SID-1 functions as a channel for the transport of double-stranded RNA (dsRNA ...
Shih, Joseph D., Hunter, Craig
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Effects of Nanoparticles on Double-Stranded RNA Stability in Corn Soil [PDF]
, 2018 Double-stranded RNA (dsRNA) can potentially be used as a pesticide because these molecules trigger an immune response called RNA interference (RNAi). If the expression of essential genes matching the dsRNA sequence are silenced, then the pest dies.
Reyanldo, Miriam +7 more
core +5 more sources
Mechanism of PKR Activation by dsRNA [PDF]
Journal of Molecular Biology, 2008 Protein kinase R (PKR) is a central component of the interferon antiviral defense pathway. Upon binding double-stranded RNA (dsRNA), PKR undergoes autophosphorylation reactions that activate the kinase. PKR then phosphorylates eukaryotic initiation factor 2alpha, thus inhibiting protein synthesis in virally infected cells.
Jeffrey W. Lary +3 more
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dsRNA Uptake in Adult Drosophila [PDF]
, 2011 RNA interference (RNAi) is a conserved sequence-specific gene silencing mechanism that is induced by double-stranded RNA (dsRNA). The development of methods that allow internalization of dsRNA and concomitant silencing of the desired gene has not stopped since the first demonstration of RNAi in Caenorhabditis elegans.
Obadia, Benjamin, Saleh, Maria-Carla
openaire +5 more sources
Capsid Structure of dsRNA Fungal Viruses [PDF]
Viruses, 2018 Most fungal, double-stranded (ds) RNA viruses lack an extracellular life cycle stage and are transmitted by cytoplasmic interchange. dsRNA mycovirus capsids are based on a 120-subunit T = 1 capsid, with a dimer as the asymmetric unit. These capsids, which remain structurally undisturbed throughout the viral cycle, nevertheless, are dynamic particles ...
José R. Castón +5 more
openaire +6 more sources