Results 111 to 120 of about 74,293 (296)

Synthetic biology approach for plant protection using dsRNA

Plant Biotechnology Journal, 2018
Summary Pathogens induce severe damages on cultivated plants and represent a serious threat to global food security. Emerging strategies for crop protection involve the external treatment of plants with double‐stranded (ds)RNA to trigger RNA interference.
Annette Niehl, Marjukka Soininen, M. Poranen, M. Heinlein   +3 more
semanticscholar   +1 more source

An RNA interference biopesticide reduces reproduction of the honey bee parasite Varroa destructor by down‐regulating embryo development pathways

Pest Management Science, EarlyView.
Varroa destructor mites exposed to dsRNA specific in sequence to a V. destructor calmodulin gene significantly effected gene expression of adult female mites sampled 5 days after entering a honey bee brood cell to reproduce. Significantly down‐regulated genes included Calmodulin and were associated with calcium ion binding and embryo developmental ...
Zoe E Smeele, Rose A McGruddy, James W Baty, Brian Manley, Kenneth Narva, Emma De Neef, Eric RL Gordon, Upendra K Devisetty, Katie Youngs, Antoine Felden, Philip J Lester   +10 more
wiley   +1 more source

Development of Biolayer Interferometry (BLI)-Based Double-Stranded RNA Detection Method with Application in mRNA-Based Therapeutics and Vaccines

Pharmaceutics
Background: In vitro-transcribed (IVT) mRNA has been established as a promising platform for therapeutics and vaccine development. Double-stranded RNA (dsRNA) is a major impurity of IVT mRNA and can trigger unfavored immune responses, potentially causing
Dharia Sara Silas, Bindiya Juneja, Keerat Kaur, Muralikrishna Narayanareddy Gari, Yingjian You, Youmi Moon, Yizhuo Chen, Srishti Arora, Johanna Hansen, Kathir Muthusamy, Yue Fu, Nisha Palackal, Erica A. Pyles   +12 more
doaj   +1 more source

Reporters transiently transfected into mammalian cells are highly sensitive to translational repression induced by dsRNA expression.

PLoS ONE, 2014
In mammals, double-stranded RNA (dsRNA) can mediate sequence-specific RNA interference, activate sequence-independent interferon response, or undergo RNA editing by adenosine deaminases. We showed that long hairpin dsRNA expression had negligible effects
Jana Nejepinska, Radek Malik, Susan Wagner, Petr Svoboda   +3 more
doaj   +1 more source

AMBRA1 promotes dsRNA- and virus-induced apoptosis through interacting with and stabilizing MAVS [PDF]

, 2021
Yuxia Lin, Changbai Huang, Huixin Gao, Xiaobo Li, Quanshi Lin, Shili Zhou, Zhiting Huo, Yanxia Huang, Chao Liu, Ping Zhang   +9 more
openalex   +1 more source

SIGS vs HIGS: a study on the efficacy of two dsRNA delivery strategies to silence Fusarium FgCYP51 genes in infected host and non‐host plants

Molecular plant pathology, 2019
Summary CYP3RNA, a double‐stranded (ds)RNA designed to concomitantly target the two sterol 14α‐demethylase genes FgCYP51A and FgCYP51B and the fungal virulence factor FgCYP51C, inhibits the growth of the ascomycete fungus Fusarium graminearum (Fg) in ...
A. Koch, L. Höfle, B. Werner, J. Imani, A. Schmidt, L. Jelonek, K. Kogel   +6 more
semanticscholar   +1 more source

Evaluating the potential of RNA interference for control of striped cucumber beetle, Acalymma vittatum (Fabricius) (Coleoptera: Chrysomelidae)

Pest Management Science, EarlyView.
SCB has a robust RNAi response to injected dsRNA, but oral RNAi is less efficient. Nucleases do not appear to be responsible for the discrepancy, suggesting other factors are involved. Abstract BACKGROUND The striped cucumber beetle (SCB) is a serious pest of cucurbit crops, causing damage both by feeding on plants and by vectoring plant diseases ...
Emine Kaplanoglu, Félix Longpré, Cam Donly   +2 more
wiley   +1 more source

Supplementary Figure 7 from Helicase-Driven Activation of NFκB-COX2 Pathway Mediates the Immunosuppressive Component of dsRNA-Driven Inflammation in the Human Tumor Microenvironment

, 2023
Marie‐Nicole Theodoraki, Saigopalakrishna S. Yerneni, Saumendra N. Sarkar, Brian J. Orr, Ravikumar Muthuswamy, Jamie Voyten, Francesmary Modugno, Weijian Jiang, Melissa Grimm, Per Basse, David L. Bartlett, Robert P. Edwards, Paweł Kaliński   +12 more
openalex   +1 more source

Glucosinolate hydrolysis products suppress entomopathogenic nematodes in vitro but do not protect sequestering flea beetle larvae in vivo

Pest Management Science, EarlyView.
The flea beetle's glucosinolate‐based chemical defense fails to protect larvae from nematode infection. However, the defense inhibits the nematode's symbiotic bacteria, thereby potentially impairing nematode reproduction and biocontrol success. Abstract BACKGROUND The efficacy of entomopathogenic nematodes (EPNs) in the biological control of insect ...
Johannes Körnig, Vojtech Beneš, Christin Manthey, Michael Reichelt, Grit Kunert, Christian Paetz, Johanna Kutzschbach, Paula Lampe, Martin Kaltenpoth, Franziska Beran   +9 more
wiley   +1 more source

Synthetic polymer nanocarrier enhances dsRNA stability but not RNAi efficacy in Brassicogethes aeneus

Pest Management Science, EarlyView.
Experimental setup for dsRNA feeding in Brassicogethes aeneus to evaluate the effect of a synthetic polymer nanocarrier on RNAi efficiency. The experiment assessed whether the nanocarrier enhances RNAi after oral dsRNA delivery. (a) Stability of dsRNA–nanocarrier complexes was tested in simulated gut conditions. (b) Gene expression of αCOP and dre4 was
Triin Kallavus, Liina Soonvald, Iris Callewaert, Ana Isabel Silva, Ericmar Ávila Dos Santos, Silva Vilumets, Riina Kaasik, Sven Vereecken, Lenny Van Daele, Peter Dubruel, Jonathan Willow, Clauvis Nji Tizi Taning, Kristof De Schutter, Eve Veromann   +13 more
wiley   +1 more source