Results 171 to 180 of about 114,219 (358)

The stiffness of dsRNA: hydrodynamic studies on fluorescence-labelled RNA segments of bovine rotavirus [PDF]

, 1986
Rüdiger Kapahnke, Winfried Rappold, Ulrich Desselberger, Detlev Riesner   +3 more
openalex   +1 more source

Generation of microalga Chlamydomonas reinhardtii expressing shrimp antiviral dsRNA without supplementation of antibiotics

Scientific Reports, 2019
RNA interference (RNAi) is an effective way of combating shrimp viruses by using sequence-specific double-stranded (dsRNA) designed to knock down key viral genes.
Patai Charoonnart, N. Worakajit, Julie A. Z. Zedler, M. Meetam, C. Robinson, V. Saksmerprome   +5 more
semanticscholar   +1 more source

New insights into control of arbovirus replication and spread by insect RNA interference pathways [PDF]

, 2012
Arthropod-borne (arbo) viruses are transmitted by vectors, such as mosquitoes, to susceptible vertebrates. Recent research has shown that arbovirus replication and spread in mosquitoes is not passively tolerated but induces host responses to control ...
Andersson, Angelini, Angelini, Arensburger, Arensburger, Attarzadeh-Yazdi, Berry, Blevins, Brackney, Chung, Cirimotich, Cohen, Czech, Ding, Dubrulle, Fagegaltier, Flynt, Franz, Ghildiyal, Henke, Holt, Hussain, Hussain, Iwasaki, Kaminker, Kawamura, Keene, Kwon, Lagos-Quintana, Li, Lim, Lucchetta, Mendes, Morazzani, Mueller, Myles, Nene, Qi, Rezza, Ruby, Saleh, Sanchez-Vargas, Schwarz, Selbach, Sijen, Siu, Skalsky, Tam, Thibault, Vodovar, Watanabe, Waterhouse, Wu, Yan, Zeiner, Zeng   +55 more
core   +3 more sources

Research progress on immune mechanism and control strategy of dsRNA impurities in mRNA vaccine

Expert Review of Vaccines
Introduction Double-stranded RNA (dsRNA) is a key impurity of mRNA vaccines prepared by in vitro transcription (IVT) and is primarily transcribed by T7 RNA polymerase.
Xinjun Liu, Chaoying Hu, Qian He, Yu Bai, Xuanxuan Zhang, Zhihao Fu, Xiao Ma, Miao Xu, Zhenglun Liang, Qunying Mao   +9 more
doaj   +1 more source

Effective target genes for RNA interference‐based management of the cabbage stem flea beetle

Insect Molecular Biology, EarlyView.
An oral RNAi screen consisting of 27 dsRNAs identified effective target genes for RNAi‐based pest control in the cabbage stem flea beetle The most effective target genes were involved in proteasomal pathways or gene expression, and their sequences were subjected to in silico off‐target analysis Knock‐down of some effective target genes also inhibited ...
Doga Cedden, Gözde Güney, Xavier Debaisieux, Stefan Scholten, Michael Rostás, Gregor Bucher   +5 more
wiley   +1 more source

Biochemical Comparison of dsRNA Degrading Nucleases in Four Different Insects

Frontiers in Physiology, 2018
Double stranded RNAs (dsRNA) degrading nuclease is responsible for the rapid degradation of dsRNA molecules, and thus accounts for variations in RNA interference (RNAi) efficacy among insect species.
Yingchuan Peng, Kangxu Wang, W. Fu, C. Sheng, Zhaojun Han   +4 more
semanticscholar   +1 more source

Effects of double-stranded RNA on virulence of Paecilomyces fumosoroseus (Deuteromycotina: Hyphomycetes) against the silverleaf whitefly, Bemisia tabaci strain B (Homoptera: Aleyrodidae)

Genetics and Molecular Biology, 2000
Bands of double-stranded RNA (dsRNA) were detected in three out of twelve isolates of Paecilomyces fumosoroseus. Identity of these bands was confirmed by RNAse, DNAse and S1 nuclease treatments.
Andréia Cristiane Souza Azevedo, Daniel Ricardo Sosa-Gómez, Marcos Rodrigues Faria, Maria Helena Pelegrinelli Fungaro   +3 more
doaj   +1 more source

The salivary gland transcriptome of Varroa destructor reveals suitable targets for RNAi‐based mite control

Insect Molecular Biology, EarlyView.
RNAseq and differential expression analysis identified 15 salivary gland genes encoding secreted proteins with putative anti‐bacterial, anti‐fungal, cytolytic, digestive and immunosuppressive function. The three most highly transcribed genes were selected to study their functional importance by reverse genetics, using a non‐invasive approach of dsRNA ...
Andrea Becchimanzi, Alfonso Cacace, Martina Parziale, Giovanna De Leva, Sergio Iacopino, Giovanni Jesu, Ilaria Di Lelio, Virgilio Stillittano, Emilio Caprio, Francesco Pennacchio   +9 more
wiley   +1 more source

Efficiency of RNA interference is improved by knockdown of dsRNA nucleases in tephritid fruit flies

Open Biology, 2019
RNA interference (RNAi) in insects is routinely used to ascertain gene function, but also has potential as a technology to control pest species. For some insects, such as beetles, ingestion of small quantities of double-stranded RNA (dsRNA) is able to ...
Alison Tayler, Daniel Heschuk, D. Giesbrecht, J. Y. Park, S. Whyard   +4 more
semanticscholar   +1 more source

The quest for the best target genes for RNAi‐mediated pest control

Insect Molecular Biology, EarlyView.
A major challenge for RNAi mediated pest control is the identification of the most effective RNAi target genes from the pool of ~37% of all genes that are essential. Effective RNAi target genes are predominantly highly conserved genes serving basic cellular functions such as protein degradation.
Doga Cedden, Gregor Bucher
wiley   +1 more source