Results 11 to 20 of about 74,293 (296)

dsRNA-Seq: Identification of Viral Infection by Purifying and Sequencing dsRNA [PDF]

Viruses, 2019
RNA viruses are a major source of emerging and re-emerging infectious diseases around the world. We developed a method to identify RNA viruses that is based on the fact that RNA viruses produce double-stranded RNA (dsRNA) while replicating. Purifying and
Carolyn J. Decker, Halley R. Steiner, Laura L. Hoon-Hanks, James H. Morrison, Kelsey C. Haist, Alex C. Stabell, Eric M. Poeschla, Thomas E. Morrison, Mark D. Stenglein, Sara L. Sawyer, Roy Parker   +10 more
doaj   +3 more sources

Mathematical modelling of OAS2 activation by dsRNA and effects of dsRNA lengths

AIMS Mathematics, 2021
The activation of 2'-5'-oligoadenylate synthetase (OAS) enzymes by direct interaction with viral double-stranded RNA (dsRNA) is a key part of the innate immune response to viral infection.
Deokro Lee, Amit Koul, Nikhat Lubna, Sean A. McKenna, Stéphanie Portet   +4 more
doaj   +2 more sources

SARS-CoV-2 nsp15 preferentially degrades AU-rich dsRNA via its dsRNA nickase activity [PDF]

Nucleic Acids Research, 2023
SUMMARYIt has been proposed that coronavirus nsp15 mediates evasion of host cell double-stranded (ds) RNA sensors via its uracil-specific endoribonuclease activity. However, how nsp15 processes viral dsRNA, commonly considered as a genome replication intermediate, remains elusive.
Xionglue Wang, Bin Zhu
openalex   +3 more sources

Specific interference by ingested dsRNA

Nature, 1998
A genetic interference phenomenon in the nematode Caenorhabditis elegans has been described in which expression of an individual gene can be specifically reduced by microinjecting a corresponding fragment of double-stranded (ds) RNA1. One striking feature of this process is a spreading effect: interference in a broad region of the animal is observed ...
L. Timmons, A. Fire
semanticscholar   +3 more sources

dsRNA-induced immunity targets plasmodesmata and is suppressed by viral movement proteins

The Plant Cell, 2023
Emerging evidence indicates that in addition to its well-recognized functions in antiviral RNA silencing, dsRNA elicits pattern-triggered immunity (PTI), likely contributing to plant resistance against virus infections. However, compared to bacterial and
Caiping Huang, A. Sede, Laura Elvira-González, Yan Yan, Miguel Rodriguez, J. Mutterer, E. Boutant, Libo Shan, M. Heinlein   +8 more
semanticscholar   +1 more source

Stress granules are shock absorbers that prevent excessive innate immune responses to dsRNA

bioRxiv, 2022
Proper defense against microbial infection depends on the controlled activation of the immune system. This is particularly important for the RIG-I-like receptors (RLRs), which recognize viral dsRNA and initiate antiviral innate immune responses with the ...
Max Paget, Cristhian Cadena, Sadeem Ahmad, Haitao Wang, Tristan X. Jordan, Ehyun Kim, Beechui Koo, Shawn M. Lyons, P. Ivanov, B. tenOever, X. Mu, S. Hur   +11 more
semanticscholar   +1 more source

A prenylated dsRNA sensor protects against severe COVID-19

Science, 2021
Description The bat connection The heterogeneity of COVID-19 makes it challenging to predict the course of infection in an individual. Upon virus infection, interferons (IFNs) generate the initial signals for cellular defenses.
Arthur Wickenhagen, Elena Sugrue, Spyros Lytras, Srikeerthana Kuchi, M. Noerenberg, Matthew L. Turnbull, C. Loney, V. Herder, Jay Allan, Innes Jarmson, Natalia Cameron-Ruiz, Margus Varjak, R. Pinto, Jeffrey Y. Lee, L. Iselin, Natasha Palmalux, Douglas G. Stewart, S. Swingler, Edward J. D. Greenwood, Thomas W M Crozier, Quan Gu, E. L. Davies, S. Clohisey, Bo Wang, Fabio Trindade Maranhão Costa, Monique Freire Santana, Luiz Carlos de Lima Ferreira, L. Murphy, A. Fawkes, A. Meynert, G. Grimes, João Da Silva Filho, M. Marti, J. Hughes, R. Stanton, E. Wang, A. Ho, Ilan Davis, R. Jarrett, Alfredo Castello, David L. Robertson, M. Semple, P. Openshaw, M. Palmarini, P. Lehner, J. Baillie, Suzannah J. Rihn, Sam J. Wilson   +47 more
semanticscholar   +1 more source

SID-1 is a dsRNA-selective dsRNA-gated channel [PDF]

RNA, 2011
Systemic RNAi in Caenorhabditis elegans requires the widely conserved transmembrane protein SID-1 to transport RNAi silencing signals between cells. When expressed in Drosophila S2 cells, C. elegans SID-1 enables passive dsRNA uptake from the culture medium, suggesting that SID-1 functions as a channel for the transport of double-stranded RNA (dsRNA ...
Shih, Joseph D., Hunter, Craig
openaire   +3 more sources

RNAi-based pest control: Production, application and the fate of dsRNA

Frontiers in Bioengineering and Biotechnology, 2022
The limitations of conventional pesticides have raised the demand for innovative and sustainable solutions for plant protection. RNA Interference (RNAi) triggered by dsRNA has evolved as a promising strategy to control insects in a species-specific ...
Li He, Yanna Huang, Xueming Tang
semanticscholar   +1 more source

Endocytosed dsRNAs induce lysosomal membrane permeabilization that allows cytosolic dsRNA translocation for Drosophila RNAi responses [PDF]

Nature Communications
RNA interference (RNAi) is a gene-silencing mechanism triggered by the cytosolic entry of double-stranded RNAs (dsRNAs). Many animal cells internalize extracellular dsRNAs via endocytosis for RNAi induction. However, it is not clear how the endocytosed dsRNAs are translocated into the cytosol across the endo/lysosomal membrane.
Tsubasa Tanaka, Tamaki Yano, Shingo Usuki, Y. H. Seo, Kento Mizuta, Maho Okaguchi, Maki Yamaguchi, Kazuko Hanyu‐Nakamura, Noriko Toyama‐Sorimachi, Katja Brückner, Akira Nakamura   +10 more
openalex   +4 more sources