Organoid and Enteroid Modeling of Salmonella Infection [PDF]
Frontiers in Cellular and Infection Microbiology, 2018 Salmonella are Gram-negative rod-shaped facultative anaerobic bacteria that are comprised of over 2,000 serovars. They cause gastroenteritis (salmonellosis) with headache, abdominal pain and diarrhea clinical symptoms. Salmonellosis brings a heavy burden
Yuebang Yin, Daoguo Zhou, Daoguo Zhou
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Molecular Basis and Differentiation-Associated Alterations of Anion Secretion in Human Duodenal Enteroid MonolayersSummary [PDF]
Cellular and Molecular Gastroenterology and Hepatology, 2018 Background & Aims: Human enteroids present a novel tool to study human intestinal ion transport physiology and pathophysiology. The present study describes the contributions of Cl- and HCO3- secretion to total cyclic adenosine monophosphate (cAMP ...
Jianyi Yin +6 more
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Apical-out bovine intestinal organoids as an infection model for Cryptosporidium parvum [PDF]
Current Research in Parasitology and Vector-Borne DiseasesCryptosporidium parvum is a major pathogen responsible for neonatal calf diarrhoea, but research has been hampered due to the lack of in vitro models that can complete the life cycle.
Mathilde Svensen Varegg +4 more
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Hypoxia Affects Stem Cell Fate in Patient-Derived Ileum Enteroids in a HIF-1α-Dependent Manner [PDF]
CellsThe intestinal epithelium maintains tissue homeostasis through a dynamic balance of stem cell proliferation and differentiation. This process is spatially regulated along the crypt–villus axis, with intestinal stem cells in the crypt regions ...
Zina M. Uckeley +13 more
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Time-Restricted Feeding Reinforces Gut Rhythmicity by Restoring Rhythms in Intestinal Metabolism in a Jetlag Mouse ModelSummary [PDF]
Cellular and Molecular Gastroenterology and HepatologyBackground & Aims: Circadian disturbances result in adverse health effects, including gastrointestinal symptoms. We investigated which physiological pathways in jejunal mucosa were disrupted during chronic jetlag and prevented during time-restricted ...
Hui Leng +5 more
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Utilizing the apical-out enteroids in vitro model to investigate intestinal glucose transport, barrier function, oxidative stress, and inflammatory responses in broiler chickens [PDF]
Frontiers in PhysiologyIntroductionConventional 2D intestinal epithelial cell lines have been widely used in investigating intestinal functions, yet with limitations in recapitulating the in vivo gut physiology of chickens.
Peter Mann +4 more
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Frontiers in Microbiology, 2023 Salmonella enterica serovar Typhimurium (STm) is a major foodborne pathogen and poultry are a key reservoir of human infections. To understand the host responses to early stages of Salmonella infection in poultry, we infected 2D and 3D enteroids, the ...
Kate Sutton +7 more
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Porcine small intestinal organoids as a model to explore ETEC–host interactions in the gut
Veterinary Research, 2021 Small intestinal organoids, or enteroids, represent a valuable model to study host–pathogen interactions at the intestinal epithelial surface. Much research has been done on murine and human enteroids, however only a handful studies evaluated the ...
Bjarne Vermeire +4 more
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TNFα Induces LGR5+ Stem Cell Dysfunction In Patients With Crohn’s DiseaseSummary
Cellular and Molecular Gastroenterology and Hepatology, 2022 Background & Aims: Tumor necrosis factor alpha (TNFα) is considered a major tissue damage-promoting effector in Crohn’s disease (CD) pathogenesis. Patient-derived intestinal organoid (enteroid) recapitulates the disease-specific characteristics of ...
Chansu Lee +6 more
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Cultivation of enteroids from fresh and cryopreserved bovine duodenal tissues
JDS Communications, 2023 This study aimed to develop a method for intestinal tissue cryopreservation and resuscitation for enteroid cultivation. Two different types of tissues, fresh duodenal tissues (n = 3, from Angus steers) and duodenal tissues cryopreserved in 90% fetal ...
Koki Nishihara +3 more
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