Results 11 to 20 of about 4,333 (201)

Establishment of bovine 3D enteroid-derived 2D monolayers [PDF]

Veterinary Research, 2022
Three-dimensional (3D) intestinal enteroids are powerful in vitro models for studying intestinal biology. However, due to their closed structure direct access to the apical surface is impeded, limiting high-throughput applications of exogenous compounds ...
Kate M. Sutton, Brigid Orr, Jayne Hope, Stina R. Jensen, Lonneke Vervelde   +4 more
doaj   +4 more sources

Human enteroids: Preclinical models of non-inflammatory diarrhea [PDF]

Stem Cell Research & Therapy, 2013
Researchers need an available and easy-to-use model of the human intestine to better understand human intestinal physiology and pathophysiology of diseases, and to offer an enhanced platform for developing drug therapy.
Broughman, J.R. (James), Donowitz, M. (Mark), Estes, M. (Mary), Ettayebi, K. (Khalil), Foulke-Abel, J. (Jennifer), Hyser, J.M. (Joseph), In, J. (Julie), Jonge, H.R. (Hugo) de, Kovbasnjuk, O. (Olga), Middendorp, S., Zachos, N.C. (Nicholas C.), Zeng, X.-L. (Xi-Lei)   +11 more
core   +4 more sources

2′-Fucosyllactose inhibits human norovirus replication in human intestinal enteroids

Journal of Virology
Human noroviruses (HuNoVs) are the leading cause of acute gastroenteritis worldwide. Currently, there are no targeted antivirals for the treatment of HuNoV infection. Histo-blood group antigens (HBGAs) on the intestinal epithelium are cellular attachment
Ketki Patil, B. Vijayalakshmi Ayyar, Nicole M. Hayes, Frederick H. Neill, Lars Bode, Mary K. Estes, Robert L. Atmar, Sasirekha Ramani   +7 more
doaj   +3 more sources

Intestinal Enteroid Culture for Human Astroviruses [PDF]

BIO-PROTOCOL, 2020
Human astroviruses (HAstV) are non-enveloped, positive-sense single stranded RNA viruses that typically cause gastroenteritis in children, the elderly and among immunocompromised individuals. Some HAstV species have also been implicated in neurological diseases.
Irene Owusu, Carmen Mirabelli, Abimbola Kolawole   +2 more
openaire   +3 more sources

Whole-mount Enteroid Proliferation Staining [PDF]

BIO-PROTOCOL, 2016
Small intestinal organoids, otherwise known as enteroids, have become an increasingly utilized model for intestinal biology in vitro as they recapitulate the various epithelial cells within the intestinal crypt (Mahe et al., 2013; Sato et al., 2009).
Caitlyn Barrett, Sarah Short, Yash Choksi, Christopher Williams   +3 more
openaire   +3 more sources

Human Sapovirus Replication in Human Intestinal Enteroids

Journal of Virology, 2023
Human sapoviruses (HuSaVs) are a frequent but overlooked cause of acute gastroenteritis, especially in children. Little is known about this pathogen, whose successful in vitro cultivation was reported only recently, in a cancer cell-derived line.
Gabriel Euller-Nicolas, Cécile Le Mennec, Julien Schaeffer, Xi-Lei Zeng, Khalil Ettayebi, Robert L. Atmar, Françoise S. Le Guyader, Mary K. Estes, Marion Desdouits   +8 more
openaire   +4 more sources

Generation of equine enteroids and enteroid-derived 2D monolayers that are responsive to microbial mimics [PDF]

Veterinary Research, 2021
AbstractEnteroid cultures are three-dimensional in vitro models that reflect the cellular composition and architecture of the small intestine. One limitation with the enteroid conformation is the enclosed lumen, making it difficult to expose the apical surface of the epithelium to experimental treatments.
openaire   +5 more sources

Bovine Enteroids as an In Vitro Model for Infection with Bovine Coronavirus

Viruses, 2023
Bovine coronavirus (BCoV) is one of the major viral pathogens of cattle, responsible for economic losses and causing a substantial impact on animal welfare.
Ruchika Shakya, Alejandro Jiménez-Meléndez, Lucy J. Robertson, Mette Myrmel   +3 more
doaj   +1 more source

The Viral Mimetic Polyinosinic:Polycytidylic Acid Alters the Growth Characteristics of Small Intestinal and Colonic Crypt Cultures. [PDF]

PLoS ONE, 2015
The intestinal epithelium is the first line of defense against enteric pathogens. We investigated the response of small intestinal and colonic crypt cultures to a panel of toll-like receptor ligands to assess the impact of microbial pattern recognition ...
Julie M Davies, Rebeca Santaolalla, Richard J von Furstenberg, Susan J Henning, Maria T Abreu   +4 more
doaj   +1 more source

Thymosin β4 dynamics during chicken enteroid development [PDF]

Molecular and Cellular Biochemistry, 2020
AbstractThe sheared avian intestinal villus-crypts exhibit high tendency to self-repair and develop enteroids in culture. Presuming that this transition process involves differential biomolecular changes, we employed matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF–MS) to find whether there were differences in the
Mohan Acharya, Rohana Liyanage, Anamika Gupta, Komala Arsi, Ann M. Donoghue, Jackson O. Lay, Narayan C. Rath   +6 more
openaire   +3 more sources