Results 11 to 20 of about 289,333 (295)

Genotyping-by-Sequencing in Plants

Biology, 2012
The advent of next-generation DNA sequencing (NGS) technologies has led to the development of rapid genome-wide Single Nucleotide Polymorphism (SNP) detection applications in various plant species.
Gregory D. May, Victor Llaca, Stéphane Deschamps   +2 more
doaj   +3 more sources

Genotyping 1000 yeast strains by next-generation sequencing [PDF]

BMC Genomics, 2013
Background The throughput of next-generation sequencing machines has increased dramatically over the last few years; yet the cost and time for library preparation have not changed proportionally, thus representing the main bottleneck for sequencing large
Wilkening Stefan, Tekkedil Manu M, Lin Gen, Fritsch Emilie S, Wei Wu, Gagneur Julien, Lazinski David W, Camilli Andrew, Steinmetz Lars M   +8 more
doaj   +4 more sources

Adapting Genotyping-by-Sequencing for Rice F2 Populations

G3: Genes, Genomes, Genetics, 2017
Rapid and cost-effective genotyping of large mapping populations can be achieved by sequencing a reduced representation of the genome of every individual in a given population, and using that information to generate genetic markers.
Tomoyuki Furuta, Motoyuki Ashikari, Kshirod K. Jena, Kazuyuki Doi, Stefan Reuscher   +4 more
doaj   +3 more sources

Genotyping-by-Sequencing for Plant Breeding and Genetics

The Plant Genome, 2012
Rapid advances in “next-generation” DNA sequencing technology have brought the US$1000 human () genome within reach while providing the raw sequencing output for researchers to revolutionize the way populations are genotyped.
Jesse A. Poland, Trevor W. Rife
doaj   +2 more sources

Improving genomic predictions by correction of genotypes from genotyping by sequencing in livestock populations [PDF]

Journal of Animal Science and Biotechnology, 2019
Background Genotyping by sequencing (GBS) is a robust method to genotype markers. Many factors can influence the genotyping quality. One is that heterozygous genotypes could be wrongly genotyped as homozygotes, dependent on the genotyping depths. In this
Xiao Wang, Mogens Sandø Lund, Peipei Ma, Luc Janss, Haja N. Kadarmideen, Guosheng Su   +5 more
doaj   +5 more sources

tGBS® genotyping-by-sequencing enables reliable genotyping of heterozygous loci [PDF]

Nucleic Acids Research, 2017
Conventional genotyping-by-sequencing (cGBS) strategies suffer from high rates of missing data and genotyping errors, particularly at heterozygous sites. tGBS® genotyping-by-sequencing is a novel method of genome reduction that employs two restriction enzymes to generate overhangs in opposite orientations to which (single-strand) oligos rather than ...
Ott, Alina, Liu, Sanzhen, Schnable, James C., Yeh, Cheng-Ting 'Eddy', Wang, Kai-Sin, Schnable, Patrick S.   +5 more
openaire   +3 more sources

Genotyping‐by‐sequencing for biogeography

Journal of Biogeography, 2022
AbstractAimGenotyping‐by‐sequencing (GBS) and similar reduced‐representation sequencing methods, such as restriction site‐associated DNA sequencing (RADseq), have been revolutionary for genetic analyses in biogeography. However, navigating the many different methodological and analytical approaches and numerous sources of potential error can be ...
Felix Vaux, Ludovic Dutoit, Ceridwen I. Fraser, Jonathan M. Waters   +3 more
openaire   +1 more source

Parentage assignment with genotyping-by-sequencing data [PDF]

Journal of Animal Breeding and Genetics, 2018
AbstractIn this paper we evaluate using genotype-by-sequencing (GBS) data to perform parentage assignment in lieu of traditional array data. The use of GBS data raises two issues: First, for low-coverage GBS data, it may not be possible to call the genotype at many loci, a critical first step for detecting opposing homozygous markers.
Andrew Whalen, Gregor Gorjanc, John M. Hickey   +2 more
openaire   +3 more sources

Characterization of the USDA Cucurbita pepo, C. moschata, and C. maxima germplasm collections

Frontiers in Plant Science, 2023
The Cucurbita genus is home to a number of economically and culturally important species. We present the analysis of genotype data generated through genotyping-by-sequencing of the USDA germplasm collections of Cucurbita pepo, C. moschata, and C. maxima.
Christopher O. Hernandez, Joanne Labate, Kathleen Reitsma, Jack Fabrizio, Kan Bao, Zhangjun Fei, Zhangjun Fei, Rebecca Grumet, Michael Mazourek   +8 more
doaj   +1 more source

Developing Methods for Maintaining Genetic Diversity in Novel Aquaculture Species: The Case of Seriola lalandi

Animals, 2023
Developing sound breeding programs for aquaculture species may be challenging when matings cannot be controlled due to communal spawning. We developed a genotyping-by-sequencing marker panel of 300 SNPs for parentage testing and sex determination by ...
Víctor Martinez, Nicolas Galarce, Alvin Setiawan   +2 more
doaj   +1 more source