Results 41 to 50 of about 81,640 (295)

Evidence for the role of proteoglycans in cation-mediated gene transfer [PDF]

, 1996
We report evidence that gene complexes, consisting of polycations and plasmid DNA enter cells via binding to membrane-associated proteoglycans. Treatment of HeLa cells with sodium chlorate, a potent inhibitor of proteoglycan sulfation, reduced luciferase
Baldeschwieler, John D., Mislick, Kimberly A.   +1 more
core   +1 more source

Adeno‐associated virus serotype 2 capsid variants for improved liver‐directed gene therapy

Hepatology, EarlyView., 2022
Abstract Background and Aims Current liver‐directed gene therapies look for adeno‐associated virus (AAV) vectors with improved efficacy. With this background, capsid engineering is explored. Whereas shuffled capsid library screenings have resulted in potent liver targeting variants with one first vector in human clinical trials, modifying natural ...
Nadja Meumann, Marti Cabanes‐Creus, Moritz Ertelt, Renina Gale Navarro, Julie Lucifora, Qinggong Yuan, Karin Nien‐Huber, Ahmed Abdelrahman, Xuan‐Khang Vu, Liang Zhang, Ann‐Christin Franke, Christian Schmithals, Albrecht Piiper, Annabelle Vogt, Maria Gonzalez‐Carmona, Jochen T. Frueh, Evelyn Ullrich, Philip Meuleman, Steven R. Talbot, Margarete Odenthal, Michael Ott, Erhard Seifried, Clara T. Schoeder, Joachim Schwäble, Leszek Lisowski, Hildegard Büning   +25 more
wiley   +1 more source

Regulation of pathologic retinal angiogenesis in mice and inhibition of VEGF-VEGFR2 binding by soluble heparan sulfate. [PDF]

PLoS ONE, 2010
Development of the retinal vascular network is strictly confined within the neuronal retina, allowing the intraocular media to be optically transparent. However, in retinal ischemia, pro-angiogenic factors (including vascular endothelial growth factor-A,
Koji M Nishiguchi, Keiko Kataoka, Shu Kachi, Keiichi Komeima, Hiroko Terasaki   +4 more
doaj   +1 more source

Heparan Sulfate Biosynthesis in Zebrafish [PDF]

Journal of Histochemistry & Cytochemistry, 2020
The biosynthesis of heparan sulfate (HS) proteoglycans occurs in the Golgi compartment of cells and will determine the sulfation pattern of HS chains, which in turn will have a large impact on the biological activity of the proteoglycans. Earlier studies in mice have demonstrated the importance of HS for embryonic development.
Lena Kjellén, Johan Ledin, Judith Habicher, Beata Filipek-Górniok   +3 more
openaire   +2 more sources

Heparan sulfate proteoglycans undergo differential expression alterations in left sided colorectal cancer, depending on their metastatic character [PDF]

, 2018
Background Heparan sulfate proteoglycans (HSPGs) are complex molecules which play a role in the invasion and growth and metastatic properties of cancerous cells.
Castañón de la Torre, Sonia, Crespo Susperregui, Ainara, Fernández Vega, Iván, García Fernández, Beatriz, García Suárez, Olivia, Quirós Fernández, Luis Manuel, Solis Hernández, María Pilar   +6 more
core   +2 more sources

Heparan Sulfate Identification and Characterisation: Method II. Enzymatic Depolymerisation and SAX-HPLC Analysis to Determine Disaccharide Composition

Bio-Protocol, 2017
Heparan sulfate (HS) is purified from complex matrices and often not fully characterised to validate its assignment. The characterisation of heparins and heparan sulfates through enzymatic depolymerisation and subsequent strong anion-exchange high ...
Susan Carnachan, Simon F.R. Hinkley
doaj   +1 more source

Proteomics Characterization of Extracellular Space Components in the Human Aorta [PDF]

, 2010
The vascular extracellular matrix (ECM) is essential for the structural integrity of the vessel wall and also serves as a substrate for the binding and retention of secreted products of vascular cells as well as molecules coming from the circulation ...
Bregant, Dahl, Deliargyris, Didangelos, Eisenstein, Eyre, Gilbert, Groves, Izano, Kalamajski, Keller, Korossis, Lampe, Layne, Lee, Lindner, Liu, Majdalawieh, Mao, Mason, Matsumoto, Mayr, Mayr, Metzler, Nakajima, Nesvizhskii, Ngo, Old, Ram, Ross, Sajdera, Schulz, Segev, Sun, Sutherland, Talusan, Tan, Waanders, Watt, Wu, Zhuang   +40 more
core   +2 more sources

Structure-Activity Relationships of Bioengineered Heparin/Heparan Sulfates Produced in Different Bioreactors

Molecules, 2017
Heparin and heparan sulfate are structurally-related carbohydrates with therapeutic applications in anticoagulation, drug delivery, and regenerative medicine.
Ha Na Kim, John M. Whitelock, Megan S. Lord   +2 more
doaj   +1 more source

Heparan sulfates and heparan sulfate proteoglycans in hematopoiesis.

Blood
Abstract From signaling mediators in stem cells to markers of differentiation and lineage commitment to facilitators for the entry of viruses, such as HIV-1, cell surface heparan sulfate (HS) glycans with distinct modification patterns play important roles in hematopoietic biology.
Piszczatowski RT, Bülow HE, Steidl U.
europepmc   +3 more sources

Heparan Sulfate Polymerization in Drosophila [PDF]

Journal of Biological Chemistry, 2006
The formation of heparan sulfate (HS) chains is catalyzed by glycosyltransferases encoded by EXT (hereditary multiple exostosin gene) family members. Genetic screening for mutations affecting morphogen signaling pathways in Drosophila has identified three genes, tout-velu (ttv), sister of tout-velu (sotv), and brother of toutvelu (botv), which encode ...
Noriyuki Egusa, Kazuyuki Sugahara, Hiroshi Kitagawa, Tomomi Izumikawa, Fumiyasu Taniguchi   +4 more
openaire   +3 more sources