Results 31 to 40 of about 131,982 (283)

A method for high precision sequencing of near full-length 16S rRNA genes on an Illumina MiSeq [PDF]

PeerJ, 2016
Background The bacterial 16S rRNA gene has historically been used in defining bacterial taxonomy and phylogeny. However, there are currently no high-throughput methods to sequence full-length 16S rRNA genes present in a sample with precision.
Catherine M. Burke, Aaron E. Darling
doaj   +2 more sources

Ultrahigh-Throughput Multiplexing and Sequencing of >500-Base-Pair Amplicon Regions on the Illumina HiSeq 2500 Platform

mSystems, 2019
Amplification, sequencing, and analysis of the 16S rRNA gene affords characterization of microbial community composition. As this tool has become more popular and amplicon-sequencing applications have grown in the total number of samples, growth in ...
Johanna B. Holm, Michael S. Humphrys, Courtney K. Robinson, Matthew L. Settles, Sandra Ott, Li Fu, Hongqiu Yang, Pawel Gajer, Xin He, Elias McComb, Patti E. Gravitt, Khalil G. Ghanem, Rebecca M. Brotman, Jacques Ravel   +13 more
doaj   +3 more sources

Bacterial diversity and community in Qula from the Qinghai–Tibetan Plateau in China [PDF]

PeerJ, 2018
Qula is a cheese-like product usually prepared with unpasteurized yak milk under open conditions, with both endogenous and exogenous microorganisms involved in the fermentation process.
Yan Zhu, Yingying Cao, Min Yang, Pengchen Wen, Lei Cao, Jiang Ma, Zhongmin Zhang, Weibing Zhang   +7 more
doaj   +2 more sources

Ambiguous results of the HLA-DPB1 genotyping results: Based on AllType NGS 11-loci sequencing reagent

Zhongguo shuxue zazhi, 2023
Objective To statistically analyze the characteristics of ambiguous results of HLA-DPB1 genotyping given by AllType NGS 11-loci sequencing reagent via two next generation sequencing platforms, i. e. Ion Torrent S5 and Illumina Miseq.
Lina DONG, Nanying CHEN, Wei WANG, Wei ZHANG, Ji HE, Faming ZHU   +5 more
doaj   +1 more source

EM-mosaic detects mosaic point mutations that contribute to congenital heart disease. [PDF]

, 2020
BackgroundThe contribution of somatic mosaicism, or genetic mutations arising after oocyte fertilization, to congenital heart disease (CHD) is not well understood.
Bernstein, Daniel, Brueckner, Martina, Chung, Wendy K, DePalma, Steven, Gelb, Bruce D, Goldmuntz, Elizabeth, Gorham, Joshua M, Griffin, Emily, Hsieh, Alexander, Kim, Richard W, Lifton, Richard P, Manheimer, Kathryn B, McKean, David, Morton, Sarah U, Newburger, Jane W, Porter, George A, Qi, Hongjian, Seidman, Christine E, Seidman, JG, Shen, Yufeng, Srivastava, Deepak, Tai, Angela C, Tristani-Firouzi, Martin, Willcox, Jon AL   +23 more
core   +3 more sources

Clinical metagenomic sequencing - CSF RNA and DNA Illumina MiSeq v1

, 2022
This protocol was developed to evaluate unbiased cerebrospinal fluid metagenomics at a referral centre in South Africa. Commercial kit protocols are generally used unmodified and limited use of in-house developed methods or reagents is made. This maintains the simplicity and accessibility of the protocol which can serve as a base for evaluation of ...
Gert Marais, Ziyaad Valley-Omar, Kathleen Bateman, Kate McMullen, Dieter van der Westhuizen, Moepeng Maseko, Suzaan Marais, Diana Hardie, Adrian Brink   +8 more
openaire   +1 more source

Systematic Comparison of Nanopore and Illumina Sequencing for the Detection of Plant Viruses and Viroids Using Total RNA Sequencing Approach

Frontiers in Microbiology, 2022
High-throughput sequencing (HTS) has become an important tool for plant virus detection and discovery. Nanopore sequencing has been rapidly developing in the recent years and offers new possibilities for fast diagnostic applications of HTS.
Anja Pecman, Anja Pecman, Ian Adams, Ion Gutiérrez-Aguirre, Adrian Fox, Neil Boonham, Maja Ravnikar, Denis Kutnjak   +7 more
doaj   +1 more source

Double-digest RADseq loci using standard Illumina indexes improve deep and shallow phylogenetic resolution of Lophodermium, a widespread fungal endophyte of pine needles. [PDF]

, 2018
The phylogenetic and population genetic structure of symbiotic microorganisms may correlate with important ecological traits that can be difficult to directly measure, such as host preferences or dispersal rates.
Oono, Ryoko, Salas-Lizana, Rodolfo
core   +1 more source

Diversity of immunoglobulin light chain genes in non-teleost ray-finned fish uncovers IgL subdivision into five ancient isotypes [PDF]

, 2018
The aim of this study was to fill important gaps in the evolutionary history of immunoglobulins by examining the structure and diversity of IgL genes in non-teleost ray-finned fish.
Andreyushkova, Daria A, Baranov, Konstantin O, Chikaev, Nikolai A, Gessner, Jörn, Guselnikov, Sergey V, Kulemzin, Sergey V, Makunin, Alexey I, Mechetina, Ludmila V, Najakshin, Alexander M, Schartl, Manfred, Stöck, Matthias, Taranin, Alexander V, Trifonov, Vladimir A, Warren, Wesley C, Wuertz, Sven   +14 more
core   +5 more sources

Analysis of the genetic diversity of influenza A viruses using next-generation DNA sequencing [PDF]

, 2015
Background: Influenza viruses exist as a large group of closely related viral genomes, also called quasispecies. The composition of this influenza viral quasispecies can be determined by an accurate and sensitive sequencing technique and data analysis ...
Saelens, Xavier, Van den Hoecke, Silvie, Verhelst, Judith, Vuylsteke, Marnik   +3 more
core   +2 more sources