mSystems, 2019 Amplification, sequencing, and analysis of the 16S rRNA gene affords characterization of microbial community composition. As this tool has become more popular and amplicon-sequencing applications have grown in the total number of samples, growth in ...
Johanna B. Holm +13 more
doaj +3 more sources
Bacterial diversity and community in Qula from the Qinghai–Tibetan Plateau in China [PDF]
PeerJ, 2018 Qula is a cheese-like product usually prepared with unpasteurized yak milk under open conditions, with both endogenous and exogenous microorganisms involved in the fermentation process.
Yan Zhu +7 more
doaj +2 more sources
Zhongguo shuxue zazhi, 2023 Objective To statistically analyze the characteristics of ambiguous results of HLA-DPB1 genotyping given by AllType NGS 11-loci sequencing reagent via two next generation sequencing platforms, i. e. Ion Torrent S5 and Illumina Miseq.
Lina DONG +5 more
doaj +1 more source
Clinical metagenomic sequencing - CSF RNA and DNA Illumina MiSeq v1
, 2022 This protocol was developed to evaluate unbiased cerebrospinal fluid metagenomics at a referral centre in South Africa. Commercial kit protocols are generally used unmodified and limited use of in-house developed methods or reagents is made. This maintains the simplicity and accessibility of the protocol which can serve as a base for evaluation of ...
Gert Marais +8 more
openaire +1 more source
Frontiers in Microbiology, 2022 High-throughput sequencing (HTS) has become an important tool for plant virus detection and discovery. Nanopore sequencing has been rapidly developing in the recent years and offers new possibilities for fast diagnostic applications of HTS.
Anja Pecman +7 more
doaj +1 more source
Diversity of immunoglobulin light chain genes in non-teleost ray-finned fish uncovers IgL subdivision into five ancient isotypes [PDF]
, 2018 The aim of this study was to fill important gaps in the evolutionary history of immunoglobulins by examining the structure and diversity of IgL genes in non-teleost ray-finned fish.
Andreyushkova, Daria A +14 more
core +5 more sources
2b-RAD genotyping for population genomic studies of Chagas disease vectors: Rhodnius ecuadoriensis in Ecuador [PDF]
, 2017 Background: Rhodnius ecuadoriensis is the main triatomine vector of Chagas disease, American trypanosomiasis, in Southern Ecuador and Northern Peru.
Andersson, Björn +10 more
core +10 more sources
Double-digest RADseq loci using standard Illumina indexes improve deep and shallow phylogenetic resolution of Lophodermium, a widespread fungal endophyte of pine needles. [PDF]
, 2018 The phylogenetic and population genetic structure of symbiotic microorganisms may correlate with important ecological traits that can be difficult to directly measure, such as host preferences or dispersal rates.
Oono, Ryoko, Salas-Lizana, Rodolfo
core +1 more source
Analysis of the genetic diversity of influenza A viruses using next-generation DNA sequencing [PDF]
, 2015 Background: Influenza viruses exist as a large group of closely related viral genomes, also called quasispecies. The composition of this influenza viral quasispecies can be determined by an accurate and sensitive sequencing technique and data analysis ...
Saelens, Xavier +3 more
core +2 more sources
Illumina MiSeq Dual-barcoded Two-step PCR Amplicon Sequencing Protocol v1
, 2020 Background Information High throughput amplicon sequencing is an extremely sensitive technique. The lab environment is full of airborne bacterial and fungal cells as well as bacterial and fungal genomic DNA from cultures, and so extreme care must be taken to keep reactions uncontaminated and in preventing cross-contamination.
Jana M. U'Ren +1 more
openaire +2 more sources