Results 41 to 50 of about 133,775 (185)

Massively parallel multiplex DNA sequencing for specimen identification using an Illumina MiSeq platform [PDF]

Scientific Reports, 2015
AbstractGenetic information is a valuable component of biosystematics, especially specimen identification through the use of species-specific DNA barcodes. Although many genomics applications have shifted to High-Throughput Sequencing (HTS) or Next-Generation Sequencing (NGS) technologies, sample identification (e.g., via DNA barcoding) is still most ...
Shadi Shokralla, Teresita M. Porter, Joel F. Gibson, Rafal Dobosz, Daniel H. Janzen, Winnie Hallwachs, G. Brian Golding, Mehrdad Hajibabaei   +7 more
openaire   +2 more sources

Diversity of cervical microbiota in asymptomatic chlamydia trachomatis genital infection: a pilot study [PDF]

, 2017
Chlamydia trachomatis genital infection continues to be an important public health problem worldwide due to its increasing incidence. C. trachomatis infection can lead to severe sequelae, such as pelvic inflammatory disease, obstructive infertility, and ...
DI PIETRO, Marisa, Farcomeni, Alessio, Filardo, Simone, Latino, Maria A, Porpora, Maria Grazia, Recine, Nadia, Sessa, Rosa   +6 more
core   +3 more sources

Advancing molecular macrobenthos biodiversity monitoring: a comparison between Oxford Nanopore and Illumina based metabarcoding and metagenomics [PDF]

PeerJ
DNA-based methods and developments of sequencing technologies are integral to macrobenthos biodiversity studies, and their implementation as standardized monitoring methods is approaching.
Karlijn Doorenspleet, Amalia Aikaterini Mailli, Berry B. van der Hoorn, Kevin K. Beentjes, Annelies De Backer, Sofie Derycke, Albertinka J. Murk, Henning Reiss, Reindert Nijland   +8 more
doaj   +2 more sources

Genomic catastrophes frequently arise in esophageal adenocarcinoma and drive tumorigenesis [PDF]

, 2014
Oesophageal adenocarcinoma (EAC) incidence is rapidly increasing in Western countries. A better understanding of EAC underpins efforts to improve early detection and treatment outcomes.
Anderson, M, Bailey, P, Barbour, A, Bruxner, T, Campbell, P, Christ, A, Fink, J, Gotley, D, Grimmond, S, Harliwong, I, Hayward, N, Holmes, O, Hussey, D, Idrisoglu, S, Kazakoff, S, Krause, L, Lampe, G, Leonard, C, Loffler, K, Lord, R, Manning, S, Miller, D, Nancarrow, D, Newell, F, Nones, K, Nourbakhsh, E, Nourse, C, Patch, A, Pearson, J, Phillips, W, Quek, K, Quinn, M, Reid, L, Simpson, P, Smithers, B, Tang, Y, Waddell, N, Waddell, N, Watson, D, Wayte, N, Whiteman, D, Wilson, P, Wood, S, Xu, Q   +43 more
core   +4 more sources

Complete genome sequences of three African foot-and-mouth disease viruses from clinical samples isolated in 2009 and 2010 [PDF]

, 2016
<p>The complete genome sequences of three foot-and-mouth disease viruses (one virus of each serotype SAT1, SAT2 and O) were directly sequenced from RNA extracted from clinical bovine samples, demonstrating the feasibility of full-genome sequencing ...
De Clercq, Kris, Fana, Mpolokang Elliot, Haegeman, Andy, Hyera, Joseph, Matlho, George, Rosseel, Toon, Seoke, Latoa, Van Borm, Steven, Vandenbussche, Frank   +8 more
core   +1 more source

Evaluating the capacity of human gut microorganisms to colonize the zebrafish larvae (Danio rerio) [PDF]

, 2018
Indexación: Scopus.In this study we evaluated if zebrafish larvae can be colonized by human gut microorganisms. We tested two strategies: (1) through transplantation of a human fecal microbiota and (2) by successively transplanting aerotolerant anaerobic
Angelica Reyes-Jara, Carmen G. Feijóo, Daniel A. Medina, Daniel Garrido, Fabien Magne, Guillermo Figueroa, Magaly Toro, Maria-Jose Valenzuela, Mario Caruffo, Maximo Coronado, Miriam Troncoso, Paola Navarrete, Salomé Muñoz, Yoani Herrera, Yoani Herrera   +14 more
core   +2 more sources

Characterization of 13 microsatellite markers for Calochortus gunnisonii (Liliaceae) from Illumina MiSeq sequencing [PDF]

Applications in Plant Sciences, 2015
Premise of the study:Microsatellite primers were designed for Calochortus gunnisonii (Liliaceae), a montane lily species of the central and southern Rocky Mountains, using next‐generation DNA sequencing. The markers will be used to investigate population structure, genetic diversity, and demographic history.Methods and Results:Thirteen polymorphic ...
Ryan S, Fuller, Seth, Frietze, Mitchell E, McGlaughlin   +2 more
openaire   +2 more sources

Comparative next-generation sequencing of adeno-associated virus inverted terminal repeats

BioTechniques, 2014
The inverted terminal repeats (ITRs) of adeno-associated virus (AAV) are notoriously difficult to sequence owing to their high GC-content (70%) and palindromic sequences that result in the formation of a very stable, 125 bp long, T-shaped hairpin ...
Karl Petri, Raffaele Fronza, Richard Gabriel, Christine Käppel, Ali Nowrouzi, R. Michael Linden, Els Henckaerts, Manfred Schmidt   +7 more
doaj   +1 more source

Rapid and economical drug resistance profiling with Nanopore MinION for clinical specimens with low bacillary burden of Mycobacterium tuberculosis

BMC Research Notes, 2020
Objective We designed and tested a Nanopore sequencing panel for direct tuberculosis drug resistance profiling. The panel targeted 10 resistance-associated loci.
Wai Sing Chan, Chun Hang Au, Yvonne Chung, Henry Chi Ming Leung, Dona N. Ho, Elaine Yue Ling Wong, Tak Wah Lam, Tsun Leung Chan, Edmond Shiu Kwan Ma, Bone Siu Fai Tang   +9 more
doaj   +1 more source

Microbial community structure of two freshwater sponges using Illumina MiSeq sequencing revealed high microbial diversity. [PDF]

AMB Express, 2016
Sponges are primitive metazoans that are known to harbour diverse and abundant microbes. All over the world attempts are being made to exploit these microbes for their biotechnological potential to produce, bioactive compounds and antimicrobial peptides.
Gaikwad S, Shouche YS, Gade WN.
europepmc   +4 more sources