Results 41 to 50 of about 131,982 (283)

Illumina MiSeq Dual-barcoded Two-step PCR Amplicon Sequencing Protocol v1

, 2020
Background Information High throughput amplicon sequencing is an extremely sensitive technique. The lab environment is full of airborne bacterial and fungal cells as well as bacterial and fungal genomic DNA from cultures, and so extreme care must be taken to keep reactions uncontaminated and in preventing cross-contamination.
Jana M. U'Ren, not provided A. Elizabeth Arnold   +1 more
openaire   +2 more sources

Massively parallel multiplex DNA sequencing for specimen identification using an Illumina MiSeq platform [PDF]

Scientific Reports, 2015
AbstractGenetic information is a valuable component of biosystematics, especially specimen identification through the use of species-specific DNA barcodes. Although many genomics applications have shifted to High-Throughput Sequencing (HTS) or Next-Generation Sequencing (NGS) technologies, sample identification (e.g., via DNA barcoding) is still most ...
Shadi Shokralla, Teresita M. Porter, Joel F. Gibson, Rafal Dobosz, Daniel H. Janzen, Winnie Hallwachs, G. Brian Golding, Mehrdad Hajibabaei   +7 more
openaire   +2 more sources

Diversity of cervical microbiota in asymptomatic chlamydia trachomatis genital infection: a pilot study [PDF]

, 2017
Chlamydia trachomatis genital infection continues to be an important public health problem worldwide due to its increasing incidence. C. trachomatis infection can lead to severe sequelae, such as pelvic inflammatory disease, obstructive infertility, and ...
DI PIETRO, Marisa, Farcomeni, Alessio, Filardo, Simone, Latino, Maria A, Porpora, Maria Grazia, Recine, Nadia, Sessa, Rosa   +6 more
core   +3 more sources

Advancing molecular macrobenthos biodiversity monitoring: a comparison between Oxford Nanopore and Illumina based metabarcoding and metagenomics [PDF]

PeerJ
DNA-based methods and developments of sequencing technologies are integral to macrobenthos biodiversity studies, and their implementation as standardized monitoring methods is approaching.
Karlijn Doorenspleet, Amalia Aikaterini Mailli, Berry B. van der Hoorn, Kevin K. Beentjes, Annelies De Backer, Sofie Derycke, Albertinka J. Murk, Henning Reiss, Reindert Nijland   +8 more
doaj   +2 more sources

Spaced seeds improve k-mer-based metagenomic classification [PDF]

, 2015
Metagenomics is a powerful approach to study genetic content of environmental samples that has been strongly promoted by NGS technologies. To cope with massive data involved in modern metagenomic projects, recent tools [4, 39] rely on the analysis of k ...
Brinda, Karel, Kucherov, Gregory, Sykulski, Maciej   +2 more
core   +2 more sources

Microbial community structure of two freshwater sponges using Illumina MiSeq sequencing revealed high microbial diversity. [PDF]

AMB Express, 2016
Sponges are primitive metazoans that are known to harbour diverse and abundant microbes. All over the world attempts are being made to exploit these microbes for their biotechnological potential to produce, bioactive compounds and antimicrobial peptides.
Gaikwad S, Shouche YS, Gade WN.
europepmc   +4 more sources

Genomic catastrophes frequently arise in esophageal adenocarcinoma and drive tumorigenesis [PDF]

, 2014
Oesophageal adenocarcinoma (EAC) incidence is rapidly increasing in Western countries. A better understanding of EAC underpins efforts to improve early detection and treatment outcomes.
Anderson, M, Bailey, P, Barbour, A, Bruxner, T, Campbell, P, Christ, A, Fink, J, Gotley, D, Grimmond, S, Harliwong, I, Hayward, N, Holmes, O, Hussey, D, Idrisoglu, S, Kazakoff, S, Krause, L, Lampe, G, Leonard, C, Loffler, K, Lord, R, Manning, S, Miller, D, Nancarrow, D, Newell, F, Nones, K, Nourbakhsh, E, Nourse, C, Patch, A, Pearson, J, Phillips, W, Quek, K, Quinn, M, Reid, L, Simpson, P, Smithers, B, Tang, Y, Waddell, N, Waddell, N, Watson, D, Wayte, N, Whiteman, D, Wilson, P, Wood, S, Xu, Q   +43 more
core   +4 more sources

Characterization of 13 microsatellite markers for Calochortus gunnisonii (Liliaceae) from Illumina MiSeq sequencing [PDF]

Applications in Plant Sciences, 2015
Premise of the study:Microsatellite primers were designed for Calochortus gunnisonii (Liliaceae), a montane lily species of the central and southern Rocky Mountains, using next‐generation DNA sequencing. The markers will be used to investigate population structure, genetic diversity, and demographic history.Methods and Results:Thirteen polymorphic ...
Ryan S, Fuller, Seth, Frietze, Mitchell E, McGlaughlin   +2 more
openaire   +2 more sources

Comparative next-generation sequencing of adeno-associated virus inverted terminal repeats

BioTechniques, 2014
The inverted terminal repeats (ITRs) of adeno-associated virus (AAV) are notoriously difficult to sequence owing to their high GC-content (70%) and palindromic sequences that result in the formation of a very stable, 125 bp long, T-shaped hairpin ...
Karl Petri, Raffaele Fronza, Richard Gabriel, Christine Käppel, Ali Nowrouzi, R. Michael Linden, Els Henckaerts, Manfred Schmidt   +7 more
doaj   +1 more source

Complete genome sequences of three African foot-and-mouth disease viruses from clinical samples isolated in 2009 and 2010 [PDF]

, 2016
<p>The complete genome sequences of three foot-and-mouth disease viruses (one virus of each serotype SAT1, SAT2 and O) were directly sequenced from RNA extracted from clinical bovine samples, demonstrating the feasibility of full-genome sequencing ...
De Clercq, Kris, Fana, Mpolokang Elliot, Haegeman, Andy, Hyera, Joseph, Matlho, George, Rosseel, Toon, Seoke, Latoa, Van Borm, Steven, Vandenbussche, Frank   +8 more
core   +1 more source