Results 51 to 60 of about 131,982 (283)

Rapid and economical drug resistance profiling with Nanopore MinION for clinical specimens with low bacillary burden of Mycobacterium tuberculosis

BMC Research Notes, 2020
Objective We designed and tested a Nanopore sequencing panel for direct tuberculosis drug resistance profiling. The panel targeted 10 resistance-associated loci.
Wai Sing Chan, Chun Hang Au, Yvonne Chung, Henry Chi Ming Leung, Dona N. Ho, Elaine Yue Ling Wong, Tak Wah Lam, Tsun Leung Chan, Edmond Shiu Kwan Ma, Bone Siu Fai Tang   +9 more
doaj   +1 more source

Evaluating the capacity of human gut microorganisms to colonize the zebrafish larvae (Danio rerio) [PDF]

, 2018
Indexación: Scopus.In this study we evaluated if zebrafish larvae can be colonized by human gut microorganisms. We tested two strategies: (1) through transplantation of a human fecal microbiota and (2) by successively transplanting aerotolerant anaerobic
Angelica Reyes-Jara, Carmen G. Feijóo, Daniel A. Medina, Daniel Garrido, Fabien Magne, Guillermo Figueroa, Magaly Toro, Maria-Jose Valenzuela, Mario Caruffo, Maximo Coronado, Miriam Troncoso, Paola Navarrete, Salomé Muñoz, Yoani Herrera, Yoani Herrera   +14 more
core   +2 more sources

Development and Validation of a Next-Generation Sequencing Assay for BRCA1 and BRCA2 Variants for the Clinical Laboratory. [PDF]

PLoS ONE, 2015
The objective of this study was to design and validate a next-generation sequencing assay (NGS) to detect BRCA1 and BRCA2 mutations. We developed an assay using random shearing of genomic DNA followed by RNA bait tile hybridization and NGS sequencing on ...
Charles M Strom, Steven Rivera, Christopher Elzinga, Taraneh Angeloni, Sun Hee Rosenthal, Dana Goos-Root, Martin Siaw, Jamie Platt, Cory Braastadt, Linda Cheng, David Ross, Weimin Sun   +11 more
doaj   +1 more source

mockrobiota: a Public Resource for Microbiome Bioinformatics Benchmarking. [PDF]

, 2016
Mock communities are an important tool for validating, optimizing, and comparing bioinformatics methods for microbial community analysis. We present mockrobiota, a public resource for sharing, validating, and documenting mock community data resources ...
Arron Shiffer, Benjamin Wolfe, Corinne F. Maurice, J. Gregory Caporaso, Jai Ram Rideout, Josh D. Neufeld, Nicholas A. Bokulich, Peter J. Turnbaugh, Rachel J. Dutton, Rob Knight, William G. Mercurio   +10 more
core   +3 more sources

A quantitative and qualitative comparison of illumina MiSeq and 454 amplicon sequencing for genotyping the highly polymorphic major histocompatibility complex (MHC) in a non-model species

BMC Research Notes, 2017
Background High-throughput sequencing enables high-resolution genotyping of extremely duplicated genes. 454 amplicon sequencing (454) has become the standard technique for genotyping the major histocompatibility complex (MHC) genes in non-model organisms.
Haslina Razali, Emily O’Connor, Anna Drews, Terry Burke, Helena Westerdahl   +4 more
doaj   +1 more source

Patient‐specific pharmacogenomics demonstrates xCT as predictive therapeutic target in colon cancer with possible implications in tumor connectivity

Molecular Oncology, EarlyView.
This study integrates transcriptomic profiling of matched tumor and healthy tissues from 32 colorectal cancer patients with functional validation in patient‐derived organoids, revealing dysregulated metabolic programs driven by overexpressed xCT (SLC7A11) and SLC3A2, identifying an oncogenic cystine/glutamate transporter signature linked to ...
Marco Strecker, Keren Zohar, Martin Böttcher, Thomas Wartmann, Henry Freudenstein, Maximilian Doelling, Mihailo Andric, Wenjie Shi, Or Kakhlon, Katrin Hippe, Beatrix Jahnke, Dimitrios Mougiakakos, Franziska Baenke, Daniel Stange, Roland S. Croner, Michal Linial, Ulf D. Kahlert   +16 more
wiley   +1 more source

Identifying Bacterial Airways Infection in Stable Severe Asthma Using Oxford Nanopore Sequencing Technologies

Microbiology Spectrum, 2022
Previous metagenomic studies in asthma have been limited by inadequate sequencing depth for species-level bacterial identification and by heterogeneity in clinical phenotyping.
Maisha F. Jabeen, Nicholas D. Sanderson, Dona Foster, Derrick W. Crook, Jennifer L. Cane, Catherine Borg, Clare Connolly, Samantha Thulborn, Ian D. Pavord, Paul Klenerman, Teresa L. Street, Timothy S. C. Hinks   +11 more
doaj   +1 more source

Reconciliation between operational taxonomic units and species boundaries [PDF]

, 2017
The development of high-throughput sequencing technologies has revolutionised the field of microbial ecology via 16S rRNA gene amplicon sequencing approaches.
Boon, Nico, Kerckhof, Frederiek-Maarten, Leys, Natalie, Monsieurs, Pieter, Mysara, Mohamed, Props, Ruben, Raes, Jeroen, Vandamme, Peter   +7 more
core   +2 more sources

High‐Throughput In Vivo Screening Using Barcoded mRNA Identifies Lipid Nanoparticles With Extrahepatic Tropism for In Situ Immunoengineering

Advanced Materials, EarlyView.
A high‐throughput in vivo mRNA LNP screening platform is developed and employed to screen a large library of 122 mRNA LNPs in vivo for delivery to immune, stromal, and parenchymal cells, identifying promising LNP candidates. A novel small particle flow cytometry‐based protein adsorption analysis method is utilized to interrogate protein corona ...
Alex G. Hamilton, Ajay S. Thatte, Junchao Xu, Zhangyi Luo, Hannah C. Safford, Kelsey L. Swingle, Jenna Muscat‐Rivera, Michael Kegel, Xuexiang Han, Ryann A. Joseph, Amanda M. Murray, Hannah C. Geisler, Ricardo C. Whitaker, Lulu Xue, Roman Spektor, Jilian R. Melamed, Drew Weissman, Michael J. Mitchell   +17 more
wiley   +1 more source

Whole Genome Sequencing of Influenza A and B Viruses With the MinION Sequencer in the Clinical Setting: A Pilot Study

Frontiers in Microbiology, 2018
Introduction: Whole genome sequencing (WGS) of influenza viruses is important for preparing vaccines and coping with newly emerging viruses. However, WGS is difficult to perform using conventional next-generation sequencers in developing countries, where
Kazuo Imai, Kazuo Imai, Kazuo Imai, Kaku Tamura, Tomomi Tanigaki, Mari Takizawa, Eiko Nakayama, Takahiko Taniguchi, Misako Okamoto, Yasumasa Nishiyama, Norihito Tarumoto, Norihito Tarumoto, Kotaro Mitsutake, Kotaro Mitsutake, Takashi Murakami, Takashi Murakami, Shigefumi Maesaki, Shigefumi Maesaki, Takuya Maeda, Takuya Maeda   +19 more
doaj   +1 more source