Results 61 to 70 of about 81,945 (302)

Reversible, Chemically Gated FRET via Ligand‐Activated Acceptors

Advanced Science, EarlyView.
Fluorogen binding turns the compact FAST tag into a tunable FRET acceptor, with ligand chemistry programming spectral overlap and excited‐state lifetimes. Fluorescence‐lifetime imaging reads out energy transfer through the shortened donor lifetime, while simply adding or washing out the dye switches FRET on and off, reversibly mapping the supramodular ...
Nivedita Singh, Smruti Ranjan Nayak, Sanjay Kumar Mohanty, Aishwarya Hanumantharaju, Amala Shaju, Mini Jose, Aravind Penmatsa, Deepak Nair   +7 more
wiley   +1 more source

Decoupling retrieval quality from generative reasoning: A multi-dimensional benchmark of RAG architectures [PDF]

EPJ Web of Conferences
Traditional search engine returns ranked lists for humans to interpret. Retrieval Augmented Generation pipelines go further, feeding retrieved context directly into large language models to synthesize knowledge rather than simply surface it.
Moutaoukkil Assmaa, El Mezouary Ali, Idarrou Ali, Boumalek Kaoutar   +3 more
doaj   +1 more source

TP53 Loss Elevates NF‐κB‐IFN‐β‐MHC‐Ia Signaling to Promote NK Cell Resistance in Osteosarcoma

Advanced Science, EarlyView.
TP53 loss in a transforming or osteosarcoma cell promotes cytosolic DNA accumulation, activating NF‐κB‐dependent IFN‐β production. Autocrine IFN‐β signaling increases cell‐surface HLA‐Ia expression, strengthens inhibitory KIR signaling in natural killer cells, and thereby enables the affected cell to evade NK cell‐mediated cytotoxicity.
Guihui Qin, Cheung Kwan Yeung, Ye Yi, Dejin Zheng, Miaoman Ye, Chi‐Chong Chio, Jinjie Wu, Siyi Fu, Chu‐Xia Deng, Ren‐He Xu   +9 more
wiley   +1 more source

PBRM1 Deficiency in Microglia Leads to Excessive Neurogenesis and Autism‐Like Behaviors via the CCL5‐CCR1/5‐β‐catenin Signaling Axis

Advanced Science, EarlyView.
Microglial PBRM1 deficiency drives excessive hippocampal neurogenesis by enhancing CCL5–CCR1/5 signaling, leading to autism‐like behaviors. A pro‐inflammatory IL1α+/TNFα+ microglial subset is identified as a key cellular contributor to this process. PBRM1 represses Ccl5 expression at the transcriptional level.
Yang‐Yang Duan, Lin‐Fei Jiao, Pei‐Pei Liu, Chun‐Hui Duan, Xiao Li, Zi‐Han Zhang, Yue‐Qi Zhang, Xin‐Li Ouyang, Hong‐Zhen Du, Sha Liu, Yi Tang, Zhao‐Qian Teng, Chang‐Mei Liu   +12 more
wiley   +1 more source

Unlocking teachers’ potential: MOOCLS, a visualization tool for enhancing MOOC teaching

Smart Learning Environments, 2023
Massive Open Online Courses (MOOCs) are revolutionizing online education and have become a popular teaching platform. However, traditional MOOCs often overlook learners' individual needs and preferences when designing learning materials and activities ...
Brahim Hmedna, Aicha Bakki, Ali El Mezouary, Omar Baz   +3 more
doaj   +1 more source

100 jaar IRF

Beleidsonderzoek Online, 2022
Onlangs vierde de Inspectie der Rijksfinanciën – beter bekend als ‘de IRF’ – haar honderdjarig bestaan. In deze column staat Peter van der Knaap stil bij het werk en de werkwijze van deze wakkere waakhond van de rijksfinanciën. Tegenwicht, tegenspraak en het maken van integrale afwegingen: dat is waar het volgens de auteur om gaat bij het streven naar ...
openaire   +1 more source

CW‐Pumped Amplified Spontaneous Emission From Thermally Evaporated CsPbBr3 Films

Advanced Science, EarlyView.
Thermally evaporated CsPbBr3 thin films sustain amplified spontaneous emission from short‐pulse toward quasi‐continuous‐wave excitation. Time‐resolved spectroscopy, controlled excitation, and substrate engineering reveal how excitation duration governs optical‐gain stability.
Yuliia Kominko, Willem Verheijen, Gabriele Rainò, Simon C. Boehme, Maksym V. Kovalenko, Sergii Yakunin   +5 more
wiley   +1 more source

IRF-3 binding to the RIG-I promoter in vivo.

, 2016
HeLa cells were transfected with poly I:C (100 ng) for 4 h and fixed; the DNA was then fragmented. Chromatin immunoprecipitation was performed using an anti-IRF-3 antibody or control IgG. The means (±SD) of three experiments are shown.
Tadaatsu Imaizumi (134869), Tomoh Matsumiya (134862), Fei Xing (134861), Ryo Hayakari (134865), Hidemi Yoshida (134872), Makoto Hayakari (3134937)   +5 more
core   +1 more source

Elevated level of SUMOylated IRF-1 in tumor cells interferes with IRF-1-mediated apoptosis [PDF]

Proceedings of the National Academy of Sciences, 2007
SUMOylation of transcription factors often attenuates transcription activity. This regulation of protein activity allows more diversity in the control of gene expression. Interferon regulatory factor-1 (IRF-1) was originally identified as a regulator of IFN-α/β, and its expression is induced by viral infection or IFN stimulation.
Junsoo, Park, Kwangsoo, Kim, Eun-Ju, Lee, Yun-Jee, Seo, Si-Nae, Lim, Kyoungsook, Park, Seung Bae, Rho, Seung-Hoon, Lee, Je-Ho, Lee   +8 more
openaire   +2 more sources

XChrom: A Cross‐Cell Chromatin Accessibility Prediction Model Integrating Genomic Sequence and Cellular Context

Advanced Science, EarlyView.
XChrom is a deep learning framework that integrates genomic sequence and single‐cell context to predict chromatin accessibility. It generalizes to unseen cells and genomic regions, enabling cross‐sample and cross‐species analysis. Applied to COVID‐19 patient data, XChrom reveals key transcriptional regulatory mechanisms and disease‐associated ...
Yuanyuan Miao, Xuan Liang, Wenwen Zhang, Dongmei Han, Yurun Li, Zhen Wang   +5 more
wiley   +1 more source