Dynamic 3D Locus Organization and Its Drivers Underpin Immunoglobulin Recombination
Frontiers in Immunology, 2021 A functional adaptive immune system must generate enormously diverse antigen receptor (AgR) repertoires from a limited number of AgR genes, using a common mechanism, V(D)J recombination.
Carolyn H. Rogers +2 more
doaj +1 more source
Human cohesin compacts DNA by loop extrusion [PDF]
Science, 2019 Cohesin extrudes DNA loops DNA is folded into loops in eukaryotic cells by a process that depends on a ring-shaped adenosine triphosphatase complex called cohesin. Davidson et al. and Kim et al.
Yoori Kim +4 more
openaire +2 more sources
3DPolyS-LE: an accessible simulation framework to model the interplay between chromatin and loop extrusion [PDF]
, 2022 International audienceAbstract Motivation Recent studies suggest that the loop extrusion activity of Structural Maintenance of Chromosomes complexes is central to proper organization of genomes in vivo .
Gitchev, Todor +7 more
core +2 more sources
Chromatin organization by an interplay of loop extrusion and compartmental segregation [PDF]
Proceedings of the National Academy of Sciences, 2017 Significance Human DNA is 2 m long and is folded into a 10-μm-sized cellular nucleus. Experiments have revealed two major features of genome organization: Segregation of alternating active and inactive regions into compartments, and formation of compacted local domains.
Johannes Nuebler +4 more
openaire +5 more sources
MoDLE: high-performance stochastic modeling of DNA loop extrusion interactions
Genome Biology, 2022 DNA loop extrusion emerges as a key process establishing genome structure and function. We introduce MoDLE, a computational tool for fast, stochastic modeling of molecular contacts from DNA loop extrusion capable of simulating realistic contact patterns ...
Roberto Rossini +4 more
doaj +1 more source
DNA double-strand break end synapsis by DNA loop extrusion. [PDF]
Nat Commun, 2023 DNA double-strand breaks (DSBs) occur every cell cycle and must be repaired. Here the authors combine theory and simulations to establish a likely role for loop extrusion in bringing the DSB ends back into proximity for ...
Yang JH, Brandão HB, Hansen AS.
europepmc +2 more sources
eLife, 2020 Cohesin has essential roles in chromosome structure, segregation and repair. Cohesin binding to chromosomes is catalyzed by the cohesin loader, Mis4 in fission yeast. How cells fine tune cohesin deposition is largely unknown.
Adrien Birot +8 more
doaj +1 more source
A complex regulatory landscape involved in the development of mammalian external genitals
eLife, 2020 Developmental genes are often controlled by large regulatory landscapes matching topologically associating domains (TADs). In various contexts, the associated chromatin backbone is modified by specific enhancer–enhancer and enhancer–promoter interactions.
Ana Rita Amândio +4 more
doaj +1 more source
Synergistic perspectives—How single‐molecule biophysics complement biochemical understanding
FEBS Letters, EarlyView.In this review, we discuss how ensemble biochemistry and single‐molecule approaches are complementary, outline commonly used single‐molecule techniques, and illustrate their relevance through two representative case studies: chromatin organization by SMC complexes and pathway choice during DNA double‐strand break repair.
Sara De Bragança +2 more
wiley +1 more source
Intrusion Effects on Maxillary Anteriors using Mini Implant Anchorage and K-Sir Loop in Subjects with Deep Overbite- A Cohort Study [PDF]
Journal of Clinical and Diagnostic Research, 2020 Introduction: Various options to treat deep bites nonsurgically involve extrusion of posterior teeth or intrusion of anterior teeth. Intrusion of anterior teeth is required in patients with excessive incisal display and to correct deep bite in adult ...
Purva Verma, Ravindra Kumar Jain
doaj +1 more source