Results 111 to 120 of about 11,326 (215)

Protocol for single-base quantification of RNA m5C by pyrosequencing

STAR Protocols
5-methylcytosine (m5C) is a common RNA modification found in both coding and non-coding RNAs. Here, we present a protocol for RNA-m5C-pyroseq for quantitative, single-nucleotide resolution analysis of RNA methylation. We describe steps for designing primers for the target region of RNA, performing bisulfite conversion of RNA, and using pyrosequencing ...
Cayir, Akin, Böttcher, Yvonne
openaire   +2 more sources

Large mammal recovery in the wake of human population decline

Journal of Applied Ecology, Volume 63, Issue 5, May 2026.
Human depopulation, particularly in rural areas, has contributed to the recovery of some large mammal populations. While recovery is generally good news for conservation, it can also lead to human‐wildlife conflict. We present several avenues to maximize coexistence in the face of further rural depopulation in other places. Read the free Plain Language
Alex J. Jensen, Benjamin R. Goldstein, Keita Fukasawa, Fabiola Iannarilli, Roland Kays   +4 more
wiley   +1 more source

Multi‐Omics Signatures of Organ Clocks in Biological Aging and Disease: A Conceptual Framework for Organ‐Specific Aging Clocks

Aging Cell, Volume 25, Issue 5, May 2026.
Organ‐specific aging clocks integrating genomics, epigenomics, transcriptomics, proteomics, and metabolomics offer a more accurate assessment of biological aging than single‐omics approaches. This review proposes a multi‐omics framework emphasizing tissue data, longitudinal study designs, and clinically meaningful training outcomes to advance the ...
Maria Vasileiou, Gabor Liposits, Bara Barakat, Nam P. Nguyen   +3 more
wiley   +1 more source

NSUN2-mediated RNA m5C modification drives multiple myeloma progression by enhancing the stability of HIP1 mRNA

Scientific Reports
RNA 5-methylcytosine (m5C) modification is a crucial epigenetic regulation, and aberrant m5C methylation is associated with the pathogenesis of certain cancers.
Yang Jiang, Jing Sun, Yuyan Chen, Lin Cheng, Saran Feng, Yan Wang, Congcong Sun   +6 more
doaj   +1 more source

Circ‐0030167/IGF2BP1 Induces Mitophagy‐Mediated Ferroptosis via HMOX1 mRNA Stabilization in Pancreatic Cancer

Cancer Science, Volume 117, Issue 5, Page 1362-1379, May 2026.
Our findings identify circ‐0030167/IGF2BP1/HMOX1 axis as a critical regulatory factor targeting mitochondria‐mediated ferroptosis, thereby presenting a novel therapeutic target for PC combination therapies. ABSTRACT Pancreatic cancer (PC) represents a highly aggressive malignancy characterized by a 5 year survival rate of less than 12%.
Qun Zhao, Xihao Yao, Jingjing Lu, Qiyang Chen, Zhiwei Wang, Xiaohong Li, Yuhua Lu   +6 more
wiley   +1 more source

Dynamical Symmetry Breaking in an E_6 GUT Model

, 1994
Dynamical symmetry breaking is studied in an E_6 GUT model of a single generation of fermions with strong 4-fermi interactions. The effective potential is analyzed analytically by the help of Michel's conjecture and the result is confirmed numerically ...
KUGO, Taichiro, SATO, Joe
core   +2 more sources

GPRC5B Identified by m5C meRIP‐Seq Suppresses Apoptosis in Osteosarcoma Through NSUN2‐Mediated RNA Methylation

Cancer Science, Volume 117, Issue 5, Page 1322-1332, May 2026.
We performed the first transcriptome‐wide profiling of m5C modifications in osteosarcoma, analyzing four pairs of tumor and adjacent normal tissues. We revealed that NSUN2 suppresses apoptosis in osteosarcoma cells by promoting GPRC5B m5C modification and expression.
Zhenyi Chen, Min Yang, Xiaoxiao Liang, Zhiqiang Yang, Wulan Mai, Yuyang Wang, Yushen Lu, Ruimin Wang, Yuanhang Han, Yuanlong Xie, Lin Cai   +10 more
wiley   +1 more source

The RNA m5C methylase NSUN4 protects retinal ganglion cells by regulating m5C methylation in the Hedgehog signaling pathway

Abstract Glaucoma, one of the leading causes of blindness worldwide, is characterized by retinal ganglion cell (RGC) damage. NOP2/Sun RNA methyltransferase 4 (NSUN4), a known 5-methylcytosine (m5C) writer, is an essential dual-function mitochondrial protein that facilitates both the process of methylation and the coordination of mitoribosome ...
Yahong Li, Dian Li, Chao Geng, Ruihua Wei, Yajian Duan   +4 more
openaire   +1 more source

Identification of m5C-related genes and subclusters in recurrent pregnancy loss

Journal of Assisted Reproduction and Genetics
Recurrent pregnancy loss (RPL), which occurs in 1-5% of couples and nearly half of the cases remain unexplained, is a complex condition influenced by multiple factors. Previous investigations have demonstrated the role of m5C-related genes (MRGs) in cancer prognosis and the significance of epigenetic modifications during pregnancy.
Huanmin Luo, Shuqing Li, Yuming Cao, Jinfeng Xu, Li Wang   +4 more
openaire   +2 more sources

The role of m5C, m1A and m7G modifications in tumors of urinary system

Frontiers in Cell and Developmental Biology
Malignant tumors of the urinary system, such as kidney cancer, bladder cancer, and prostate cancer, remain a significant challenge despite the various treatment options available. Identifying therapeutic targets for urological tumors is crucial due to the potential for recurrence and metastasis.
Wei Xue, Yuanyuan Zhao, Gong Zhang, Zhiyuan Li, Jixin Li, Xiang Fei   +5 more
openaire   +4 more sources