Results 131 to 140 of about 4,909 (142)

Target-Specific Profiling of RNA m5C Methylation Level Using Amplicon Sequencing

2021
Mapping the position and quantifying the level of 5-methylcytosine (m5C) as a modification in different types of cellular RNA is an important objective in the field of epitranscriptomics. Bisulfite conversion has long been the gold standard for the detection of m5C in DNA, but it can also be applied to RNA.
Tennille, Sibbritt, Ulrike, Schumann, Andrew, Shafik, Marco, Guarnacci, Susan J, Clark, Thomas, Preiss   +5 more
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DNA methyltransferase 1 modulates mitochondrial function through bridging m5C RNA methylation [PDF]

Molecular Cell
DNA methyltransferase 1 (DNMT1) is an enzyme known for DNA methylation maintenance. Point mutations in its replication focus targeting sequence (RFTS) domain lead to late-onset neurodegeneration, such as autosomal dominant cerebellar ataxia-deafness and narcolepsy (ADCA-DN) disorder.
Jing Wang, Xiaoqian Deng, Tianshen Jian, Shanshan Yin, Linzhi Chen, Laurent Vergnes, Zhehao Li, Huoyuan Liu, Ryan Lee, Sin Yee Lim, Jae Hoon Bahn, Xinshu Xiao, Xianmin Zhu, Ganlu Hu, Karen Reue, Yizhi Liu, Guoping Fan   +16 more
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NSUN2-Mediated m5C Methylation Impairs Endometrial Receptivity

Laboratory Investigation
Impaired endometrial decidualization is the primary cause of recurrent implantation failure (RIF). RNA methylation modification, especially NSUN family mediated m5C, is crucial for various physiological events, such as maternal-to-zygotic transition, gametogenesis, embryonic development, organismal lifespan, and cell cycle.
Jiafeng Lu, Ming Zhang, Zhenxing Liu, Ling Guo, Peng Huang, Wenjuan Xia, Jincheng Li, Jinghuan Lv, Hoi-Hung Cheung, Chenyue Ding, Hong Li, Boxian Huang   +11 more
openaire   +2 more sources

RNA methylation and breast cancer: insights into m6A, m7G and m5C

Molecular Biology Reports
Breast cancer remains the most commonly diagnosed cancer in female worldwide, marked by its molecular diversity and complex subtypes. Despite progress in targeted therapies, tumor heterogeneity and treatment resistance continue to present major challenges. Recent studies emphasize the crucial role of RNA modifications in cancer biology, with nearly 200
Yuhan, Dai, Shuhan, Zhao, Huilin, Chen, Wenxin, Yu, Ziyi, Fu, Yangyang, Cui, Hui, Xie   +6 more
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Methylated RNA Immunoprecipitation Assay to Study m5C Modification in Arabidopsis.

Journal of visualized experiments : JoVE, 2020
Secondary base modifications on RNA, such as m5C, affect the structure and function of the modified RNA molecules. Methylated RNA Immunoprecipitation and sequencing (MeRIP-seq) is a method that aims to enrich for methylated RNA and ultimately identify modified transcripts.
Saplaoura, Eleftheria, Perrera, Valentina, Colot, Vincent, Kragler, Friedrich   +3 more
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Exploration of the Prognostic Value of m5C Methylation Protein NOP2 and NSUN6 in Colon Cancer

Genetic Testing and Molecular Biomarkers
Background: Colorectal cancer is a prevalent malignancy with high incidence and poor prognosis. This study explores the clinical significance of 5-methylcytosine RNA modification factors, specifically the NOP2/Sun RNA Methyltransferase (NSUN) family, in colorectal cancer.
Bin Jiang, Jie Li, Jianguo Wang
openaire   +2 more sources

The role of m5C RNA methylation regulators in the diagnosis and immune microenvironment of osteoarthritis

Computer Methods in Biomechanics and Biomedical Engineering
The 5-methylcytosine (m5C) is a common post-transcriptional RNA methylation modification and is involved in the pathological process of many diseases. However, little is known about the role of m5C in osteoarthritis (OA). OA gene data and the corresponding information were downloaded from the Gene Expression Omnibus database. Based on 36 m5C regulators,
Kehan, Li, Shengjie, Wang, Chenyue, Xu, Zhengyi, Ni, Xiurong, Wang, Fei, Wang   +5 more
openaire   +2 more sources

Nucleotide-Level Profiling of m5C RNA Methylation

2016
Tennille Sibbritt, Andrew Shafik, Susan J. Clark, Thomas Preiss   +3 more
openaire   +1 more source

Erratum: Methylated RNA Immunoprecipitation Assay to Study m5C Modification in Arabidopsis.

Journal of visualized experiments : JoVE
An erratum was issued for: Methylated RNA Immunoprecipitation Assay to Study m5C Modification in Arabidopsis. The author list was updated. The author list was updated from: Eleftheria Saplaoura1, Valentina Perrera2, Friedrich Kragler1 1Max Planck Institute of Molecular Plant Physiology 2Department of Molecular Medicine, Medical School, University of ...
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Dynamic landscape and regulation of m5C methylation in human tissues

Science China Life Sciences
Ke An, Ruijuan Liu, Han Yang, Luyao Feng, Xinhan Zhang, Xuran Zhang, Mingyang Hou, Jingyao Wei, Xiao Han, Boyang Shi, Huan Yao, Xing Chen, Han Wang, Xin Tian   +13 more
openaire   +1 more source