Screening Phosphorylation Site Mutations in Yeast Acetyl-CoA Carboxylase Using Malonyl-CoA Sensor to Improve Malonyl-CoA-Derived Product [PDF]
Frontiers in Microbiology, 2018 Malonyl-coenzyme A (malonyl-CoA) is a critical precursor for the biosynthesis of a variety of biochemicals. It is synthesized by the catalysis of acetyl-CoA carboxylase (Acc1p), which was demonstrated to be deactivated by the phosphorylation of Snf1 ...
Jin Hou, Hou Jin
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Expression of Yarrowia lipolytica acetyl-CoA carboxylase in Saccharomyces cerevisiae and its effect on in-vivo accumulation of Malonyl-CoA [PDF]
Computational and Structural Biotechnology Journal, 2022 Malonyl-CoA is an energy-rich molecule formed by the ATP-dependent carboxylation of acetyl coenzyme A catalyzed by acetyl-CoA carboxylase. This molecule is an important precursor for many biotechnologically interesting compounds such as flavonoids ...
Humberto Pereira +3 more
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Alternative malonyl-CoA pathways for microbial production of diverse products [PDF]
Applied Microbiology and BiotechnologyMalonyl-CoA serves as a central precursor for the biosynthesis of diverse high-value compounds, including lipids, organic acids, and polyketides, in engineered microbial fermentation systems.
Kian Ghaempanah +2 more
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Structural basis of a bi-functional malonyl-CoA reductase (MCR) from the photosynthetic green non-sulfur bacterium Roseiflexus castenholzii [PDF]
mBio, 2023 Malonyl-CoA reductase (MCR) is a NADPH-dependent bi-functional enzyme that performs alcohol dehydrogenase and aldehyde dehydrogenase (CoA-acylating) activities in the N- and C-terminal fragments, respectively.
Xin Zhang +10 more
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Gap junctions deliver malonyl-CoA from soma to germline to support embryogenesis in Caenorhabditis elegans [PDF]
eLife, 2020 Gap junctions are ubiquitous in metazoans and play critical roles in important biological processes, including electrical conduction and development. Yet, only a few defined molecules passing through gap junction channels have been linked to specific ...
Todd A Starich +2 more
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Enhanced biosynthesis of poly(3‐hydroxybutyrate) in engineered strains of Pseudomonas putida via increased malonyl‐CoA availability [PDF]
Microbial BiotechnologyMalonyl‐coenzyme A (CoA) is a key precursor for the biosynthesis of multiple value‐added compounds by microbial cell factories, including polyketides, carboxylic acids, biofuels, and polyhydroxyalkanoates.
Giusi Favoino +4 more
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Engineering Yarrowia lipolytica to Enhance the Production of Malonic Acid via Malonyl‐CoA Pathway at High Titer [PDF]
Advanced ScienceMalonic acid (MA) is a high‐value‐added chemical with significant applications in the polymers, pharmaceutical, and food industries. Microbial production of MA presents enzyme inefficiencies, competitive metabolic pathways, and dispersive carbon flux ...
Qun Yang +4 more
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Biosensor-aided high-throughput screening of hyper-producing cells for malonyl-CoA-derived products [PDF]
Microbial Cell Factories, 2017 Background Malonyl-coenzyme A (CoA) is an important biosynthetic precursor in vivo. Although Escherichia coli is a useful organism for biosynthetic applications, its malonyl-CoA level is too low.
Heng Li +4 more
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Acetyl- and malonyl-CoA availability drive EPA selectivity in polyketide synthase-engineered Yarrowia lipolytica [PDF]
Microbial Cell FactoriesBackground The oleaginous yeast Yarrowia lipolytica is an attractive chassis for sustainable production of long‑chain ω‑3 polyunsaturated fatty acids (PUFAs).
Hang Qi +7 more
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Malonyl CoA Decarboxylase Inhibition Improves Cardiac Function Post-Myocardial Infarction [PDF]
JACC: Basic to Translational Science, 2019 Summary: Alterations in cardiac energy metabolism after a myocardial infarction contribute to the severity of heart failure (HF). Although fatty acid oxidation can be impaired in HF, it is unclear if stimulating fatty acid oxidation is a desirable ...
Wei Wang, MD, PhD +22 more
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