Results 1 to 10 of about 19,503 (138)

Characterising nanobody developability to improve therapeutic design using the Therapeutic Nanobody Profiler [PDF]

Communications Biology
Developability optimisation is an important step for successful biotherapeutic design. For monoclonal antibodies, developability is relatively well characterised. However, progress for novel biotherapeutics such as nanobodies is more limited. Differences
Gemma L. Gordon, João Gervasio, Colby Souders, Charlotte M. Deane   +3 more
doaj   +2 more sources

Cell-Based Immunization Combined with Single-Round Cell Panning Enables Discovery of PSMA-Targeting Nanobodies from Phage Display Libraries [PDF]

Biomolecules
There is a strong need for nanobodies that target novel cancer-associated antigens to advance radioligand imaging and antibody-based therapeutics. In this study, we investigated whether non-targeted llama immunization using tumor cells, combined with non-
Tong Yang, Joke Veldhoven-Zweistra, Maarten Ligtenberg, Sigrun Erkens, Mirella Vredenbregt-van den Berg, Rick Jansen, Patrick Chames, Eric M. J. Bindels, Khadijeh Ahmadi, Chris H. Bangma, Anton M. F. Kalsbeek, Janne Leivo, Nicolaas Lumen, Harmen J. G. van de Werken, Wytske M. van Weerden, Soudabeh Kavousipour, Raheleh Tooyserkani, Guido Jenster   +17 more
doaj   +2 more sources

Nanobody Therapeutics in Alzheimer’s Disease: From Molecular Mechanisms to Translational Approaches [PDF]

Antibodies
Nanobodies (single-domain antibodies, VHHs) have emerged as versatile tools for evaluating and treating Alzheimer’s disease (AD). They offer distinct engineering benefits compared with traditional antibodies and small molecules, including small size ...
Deepika Godugu, Kranthi Gattu, Parul Suri, Abel B. Daartey, Krishna Jadhav, Satish Rojekar   +5 more
doaj   +2 more sources

Effective targeting of microglial P2X7 following intracerebroventricular delivery of nanobodies and nanobody-encoding AAVs

Frontiers in Pharmacology, 2022
The P2X7 ion channel is a key sensor for extracellular ATP and a key trigger of sterile inflammation. Intravenous injection of nanobodies that block P2X7 has shown to be beneficial in mouse models of systemic inflammation.
Carolina Pinto-Espinoza, Carolina Pinto-Espinoza, Charlotte Guillou, Björn Rissiek, Maximilian Wilmes, Ehsan Javidi, Nicole Schwarz, Nicole Schwarz, Marten Junge, Friedrich Haag, Nastassia Liaukouskaya, Nicola Wanner, Annette Nicke, Catelijne Stortelers, Yossan-Var Tan, Sahil Adriouch, Tim Magnus, Friedrich Koch-Nolte   +17 more
doaj   +1 more source

Construction of an alpaca immune antibody library for the selection of nanobodies against Drosophila melanogaster proteins

Frontiers in Bioengineering and Biotechnology, 2023
Introduction:Drosophila melanogaster is a model organism for studying developmental biology and human neural disorders. Nanobodies are the variable domains of the heavy chains of camelid heavy-chain antibodies (VHHs) with high affinity to their antigens ...
Jianxiang Qiu, Jie Li, Zhen Zhang, Shirui Dong, Xiaomei Ling, Zhixin Fang, Quanshou Ling, Zhixin Huang   +7 more
doaj   +1 more source

Development and Characterization of Nanobodies Targeting the Kupffer Cell

Frontiers in Immunology, 2021
Nanobodies that are derived from single-chain antibodies of camelids have served as powerful tools in diagnostics, therapeutics and investigation of membrane receptors' structure and function. In this study, we developed a series of nanobodies by a phage
Fang Zheng, Fang Zheng, Fang Zheng, Jinhong Zhou, Jinhong Zhou, Zhenlin Ouyang, Jiaxin Zhang, Xinyi Wang, Serge Muyldermans, Jo Van Ginderachter, Jo Van Ginderachter, Nick Devoogdt, Yurong Wen, Yurong Wen, Steve Schoonooghe, Steve Schoonooghe, Geert Raes, Geert Raes   +17 more
doaj   +1 more source

The tax-inducible actin-bundling protein fascin is crucial for release and cell-to-cell transmission of human T-cell leukemia virus type 1 (HTLV-1) [PDF]

, 2016
The delta-retrovirus Human T-cell leukemia virus type 1 (HTLV-1) preferentially infects CD4(+) T-cells via cell-to-cell transmission. Viruses are transmitted by polarized budding and by transfer of viral biofilms at the virological synapse (VS ...
Gettemans, Jan, Gross, Christine, Kalmer, Martina, Millen, Sebastian, Thoma-Kress, Andrea K, Wiesmann, Veit, Wittenberg, Thomas   +6 more
core   +16 more sources

Influence of nanobody binding on fluorescence emission, mobility, and organization of GFP-tagged proteins

iScience, 2021
Summary: Advanced fluorescence microscopy studies require specific and monovalent molecular labeling with bright and photostable fluorophores. This necessity led to the widespread use of fluorescently labeled nanobodies against commonly employed ...
Falk Schneider, Taras Sych, Christian Eggeling, Erdinc Sezgin   +3 more
doaj   +1 more source

Quantitative flow cytometric selection of tau conformational nanobodies specific for pathological aggregates

Frontiers in Immunology, 2023
Single-domain antibodies, also known as nanobodies, are broadly important for studying the structure and conformational states of several classes of proteins, including membrane proteins, enzymes, and amyloidogenic proteins.
Jennifer M. Zupancic, Jennifer M. Zupancic, Matthew D. Smith, Matthew D. Smith, Hanna Trzeciakiewicz, Mary E. Skinner, Sean P. Ferris, Emily K. Makowski, Emily K. Makowski, Michael J. Lucas, Michael J. Lucas, Michael J. Lucas, Nikki McArthur, Ravi S. Kane, Henry L. Paulson, Henry L. Paulson, Henry L. Paulson, Peter M. Tessier, Peter M. Tessier, Peter M. Tessier, Peter M. Tessier, Peter M. Tessier, Peter M. Tessier   +22 more
doaj   +1 more source

Nanobody mediated inhibition of attachment of F18 fimbriae expressing Escherichia coli [PDF]

, 2014
Post-weaning diarrhea and edema disease caused by F18 fimbriated E. coli are important diseases in newly weaned piglets and lead to severe production losses in farming industry.
Coddens, Annelies, Cox, Eric, De Greve, Henri, De Kerpel, Maia, Moonens, Kristof, Pardon, Els, Remaut, Han   +6 more
core   +8 more sources