Results 21 to 30 of about 19,553 (185)
Domain swapping of complementarity-determining region in nanobodies produced by Pichia pastoris
AMB Express, 2019 Easy preparation of chimeric nanobodies with various scaffolds is important for customizing abilities of nanobodies toward practical utilization. To accomplish high-throughput production of various nanobodies, utilization of microbes is an attractive ...
Natsuko Miura +7 more
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Facile isolation of high-affinity nanobodies from synthetic libraries using CDR-swapping mutagenesis
STAR Protocols, 2022 Summary: The generation of high-affinity nanobodies for diverse biomedical applications typically requires immunization or affinity maturation. Here, we report a simple protocol using complementarity-determining region (CDR)-swapping mutagenesis to ...
Jennifer M. Zupancic +2 more
doaj +1 more source
Identification and characterization of nanobodies targeting the EphA4 receptor [PDF]
, 2017 The ephrin receptor A4 (EphA4) is one of the receptors in the ephrin system that plays a pivotal role in a variety of cell-cell interactions, mostly studied during development. In addition, EphA4 has been found to play a role in cancer biology as well as
Chavez-Gutierrez, Lucia +16 more
core +2 more sources
Recent advances of nanobody applications in diagnosis and detection
MedComm – Biomaterials and Applications, 2023 Nanobodies (Nbs) are the variable domain of heavy‐chain antibodies derived from the blood of camelids or sharks. Nanobodies are the smallest antibody fragment with intact antigen‐binding ability.
Qianling Su +5 more
doaj +1 more source
Chemogenetic Control of Nanobodies [PDF]
Nature Methods, 2019 ABSTRACTWe introduce an engineered nanobody whose affinity to green fluorescent protein (GFP) can be switched on and off with small molecules. By controlling the cellular localization of GFP fusion proteins, the engineered nanobody allows to study their role in basic biological processes, an approach that should be applicable to numerous previously ...
Farrants, Helen +9 more
openaire +3 more sources
Scientific Reports, 2021 Enterotoxigenic Escherichia coli (ETEC) is estimated to cause approximately 380,000 deaths annually during sporadic or epidemic outbreaks worldwide.
Alla Amcheslavsky +18 more
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Nanobodies as tools to understand, diagnose, and treat African trypanosomiasis [PDF]
, 2017 African trypanosomes are strictly extracellular protozoan parasites that cause diseases in humans and livestock and significantly affect the economic development of sub-Saharan Africa.
Caljon, Guy +5 more
core +1 more source
NANOBODIES: STRUCTURE, MANUFACTURING, APPLICATION (REVIEW)
Разработка и регистрация лекарственных средств, 2019 Introduction. Single-domain antibodies (nanobodies) are composed of the heavy-chain variable domain only. Compared to conventional immunoglobulins G (IgG) nanobodies have such qualities as: high bioavailability, ability to bind epitopes that are ...
A. V. Shatalova +3 more
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A general approach for stabilizing nanobodies for intracellular expression
eLife, 2022 Conventional antibodies and their derived fragments are difficult to deploy against intracellular targets in live cells, due to their bulk and structural complexity.
John G Dingus +4 more
doaj +1 more source
Oligoclonal selection of nanobodies targeting vascular endothelial growth factor
Journal of Immunotoxicology, 2019 While monoclonal antibodies are efficient therapeutics for cancer treatment, nanobodies or variable heavy domain – due to their small size, high stability, and solubility – have many advantages in comparison.
Mehrdad Ahadi +3 more
doaj +1 more source