Results 21 to 30 of about 9,815 (163)
PLoS ONE, 2014 We have evaluated suitable reference genes for real time (RT)-quantitative PCR (qPCR) analysis in Japanese pear (Pyrus pyrifolia). We tested most frequently used genes in the literature such as β-Tubulin, Histone H3, Actin, Elongation factor-1α ...
Tsuyoshi Imai +3 more
doaj +1 more source
Heliyon, 2023 Objective: To screen and validate reference genes suitable for gene mRNA expression study in peripheral blood mononuclear cells (PBMCs) between septic patients and healthy controls (HC).
Ruoyu Song +10 more
doaj +1 more source
Reference genes for normalization of qPCR assays in sugarcane plants under water deficit
Plant Methods, 2017 Background Sugarcane (Saccharum spp.) is the main raw material for sugar and ethanol production. Among the abiotic stress, drought is the main one that negatively impact sugarcane yield.
Larissa Mara de Andrade +7 more
doaj +1 more source
BMC Research Notes, 2011 Findings At first 32 housekeeping genes were analyzed in six randomly chosen meningiomas, brain and dura mater using geNorm, NormFinder, Bestkeeper-1 software and the comparative ΔCt method.
Pfister Christina +2 more
doaj +1 more source
BMC Genomics, 2017 Background In order to better understand the multifactorial nature of osteoporosis, animal models are utilized and compared to healthy controls. Female sheep are well established as a model for osteoporosis induced by ovariectomy, calcium and vitamin D ...
Felix Schulze +6 more
doaj +1 more source
Validation of reference genes for the normalization of the RT-qPCR in peripheral blood mononuclear cells of patients with Takayasu arteritis [PDF]
Jichu yixue yu linchuang, 2021 Objective To validate proper reference genes for quantitative real-time polymerase chain reaction (RT-qPCR) used for comparing mRNA expression levels in Takayasu arteritis' (TAK) and healthy controls' (HC) peripheral blood mononuclear cells (PBMC ...
TIAN Yi-xiao, LI Jing
doaj
Selection and validation of a set of reliable reference genes for quantitative RT-PCR studies in the brain of the Cephalopod Mollusc [PDF]
, 2009 Background: Quantitative real-time polymerase chain reaction (RT-qPCR) is valuable for studying the molecular events underlying physiological and behavioral phenomena.
Maria Sirakov +6 more
core +1 more source
Gene expression studies: How to obtain accurate and reliable data by quantitative real-time RT PCR [PDF]
Journal of Medical Biochemistry, 2013 Real-time RT PCR has been recognized as an accurate, reliable and sensitive method for quantifying gene transcription. However, several steps preceding PCR represent critical points and source of inaccuracies.
Nestorov Jelena +3 more
doaj
Selection of reference genes for gene expression studies in Siberian Apricot (Prunus sibirica L.) Germplasm using quantitative real-time PCR. [PDF]
PLoS ONE, 2014 Quantitative real time reverse transcription polymerase chain reaction has been applied in a vast range of studies of gene expression analysis. However, real-time PCR data must be normalized with one or more reference genes.
Jun Niu +11 more
doaj +1 more source
Reference loci for RT-qPCR analysis of differentiating human embryonic stem cells [PDF]
, 2013 Background: Selecting stably expressed reference genes is essential for proper reverse transcription quantitative polymerase chain reaction gene expression analysis. However, this choice is not always straightforward. In the case of differentiating human
De Sutter, Petra +6 more
core +2 more sources