PLoS ONE, 2014 We have evaluated suitable reference genes for real time (RT)-quantitative PCR (qPCR) analysis in Japanese pear (Pyrus pyrifolia). We tested most frequently used genes in the literature such as β-Tubulin, Histone H3, Actin, Elongation factor-1α ...
Tsuyoshi Imai +3 more
doaj +1 more source
Heliyon, 2023 Objective: To screen and validate reference genes suitable for gene mRNA expression study in peripheral blood mononuclear cells (PBMCs) between septic patients and healthy controls (HC).
Ruoyu Song +10 more
doaj +1 more source
Reference genes for normalization of qPCR assays in sugarcane plants under water deficit
Plant Methods, 2017 Background Sugarcane (Saccharum spp.) is the main raw material for sugar and ethanol production. Among the abiotic stress, drought is the main one that negatively impact sugarcane yield.
Larissa Mara de Andrade +7 more
doaj +1 more source
BMC Research Notes, 2011 Findings At first 32 housekeeping genes were analyzed in six randomly chosen meningiomas, brain and dura mater using geNorm, NormFinder, Bestkeeper-1 software and the comparative ΔCt method.
Pfister Christina +2 more
doaj +1 more source
Validation of reference genes for the normalization of the RT-qPCR in peripheral blood mononuclear cells of patients with Takayasu arteritis [PDF]
Jichu yixue yu linchuang, 2021 Objective To validate proper reference genes for quantitative real-time polymerase chain reaction (RT-qPCR) used for comparing mRNA expression levels in Takayasu arteritis' (TAK) and healthy controls' (HC) peripheral blood mononuclear cells (PBMC ...
TIAN Yi-xiao, LI Jing
doaj
Gene expression studies: How to obtain accurate and reliable data by quantitative real-time RT PCR [PDF]
Journal of Medical Biochemistry, 2013 Real-time RT PCR has been recognized as an accurate, reliable and sensitive method for quantifying gene transcription. However, several steps preceding PCR represent critical points and source of inaccuracies.
Nestorov Jelena +3 more
doaj
Selection of reference genes for gene expression studies in Siberian Apricot (Prunus sibirica L.) Germplasm using quantitative real-time PCR. [PDF]
PLoS ONE, 2014 Quantitative real time reverse transcription polymerase chain reaction has been applied in a vast range of studies of gene expression analysis. However, real-time PCR data must be normalized with one or more reference genes.
Jun Niu +11 more
doaj +1 more source
Analysis of qPCR reference gene stability determination methods and a practical approach for efficiency calculation on a turbot (Scophthalmus maximus) gonad dataset [PDF]
, 2014 Background Gene expression analysis by reverse transcription quantitative PCR (qPCR) is the most widely used method for analyzing the expression of a moderate number of genes and also for the validation of microarray results.
Ana Viñas +6 more
core +4 more sources
Selection of housekeeping genes for gene expression studies in the adult rat submandibular gland under normal, inflamed, atrophic and regenerative states [PDF]
, 2008 Background: Real-time PCR is a reliable tool with which to measure mRNA transcripts, and provides valuable information on gene expression profiles. Endogenous controls such as housekeeping genes are used to normalise mRNA levels between samples for ...
Emanuele Cotroneo +5 more
core +2 more sources
Validation of control genes and a standardised protocol for quantifying gene expression in the livers of C57BL/6 and ApoE−/− mice [PDF]
, 2018 The liver plays a critical role in food and drug metabolism and detoxification and accordingly influences systemic body homeostasis in health and disease.
AN Heinloth +24 more
core +1 more source