Targeted online liquid chromatography electron capture dissociation mass spectrometry for the localization of sites of in vivo phosphorylation in human Sprouty2 [PDF]
, 2008 We demonstrate a strategy employing collision-induced dissociation for phosphopeptide discovery, followed by targeted electron capture dissociation (ECD) for site localization. The high mass accuracy and low background noise of the ECD mass spectra allow
Abbott K. L. +42 more
core +1 more source
Antigenic Determinant of Trichosanthin Peptide Fragments
Immunopharmacology and Immunotoxicology, 1986 Purified plant protein, Trichosanthin (TCS), was partially digested with chymotrypsin to produce peptide fragments. Two large fragments, I and II, have been isolated and identified as C-terminal peptides located at sequence positions between 79-234 and 107-234. Their molecular weights determined by electrophoretic mobility on SDS gel were 16,000 and 14,
Zhen Yao +3 more
openaire +4 more sources
Collisional energy dependence of peptide ion fragmentation [PDF]
Journal of the American Society for Mass Spectrometry, 2009 The energy dependence of fragmentation in a collision cell was measured for 2400 protonated peptide ions derived from the digestion of 24 proteins. The collision voltage at which the sum of the fragment ion abundances was equal to the remaining parent ion (V(1/2)) was the principal measure of fragmentation effectiveness.
Stephen E. Stein +3 more
openaire +3 more sources
Improved Detection and Fragmentation of Disulphide-Linked Peptides [PDF]
Methods and Protocols, 2018 Characterisation of peptides containing intact disulphide bonds (DSBs) via mass spectrometry is challenging. Our study demonstrates that the addition of aniline to alpha-cyano-4-hydroxycinnamic acid improves detection and fragmentation of complex DSB peptides by matrix-assisted laser desorption/ionization, tandem time-of-flight mass spectrometry (MALDI-
Santanu Deb-Choudhury +3 more
openaire +2 more sources
Liquid Chromatography Electron Capture Dissociation Tandem Mass Spectrometry (LC-ECD-MS/MS) versus Liquid Chromatography Collision-induced Dissociation Tandem Mass Spectrometry (LC-CID-MS/MS) for the Identification of Proteins [PDF]
, 2007 Electron capture dissociation (ECD) offers many advantages over the more traditional fragmentation techniques for the analysis of peptides and proteins, although the question remains: How suitable is ECD for incorporation within proteomic strategies for ...
A. G. Marshall +38 more
core +4 more sources
NEXAFS spectroscopy and site-specific fragmentation of N -methylformamide, N,N -dimethylformamide, and N,N -dimethylacetamide [PDF]
, 2016 Near-edge X-ray absorption fine-structure (NEXAFS) spectra measured at the C, N, and O K-edges for three molecules containing the amide moiety, N-methylformamide (HCONHCH3), N,Ndimethylformamide (HCON(CH3)2), and N,N-dimethylacetamide (CH3CON(CH3)2 ...
Alagia, Michele +7 more
core +1 more source
Photocatalytic Redox Reactions for In‐Source Peptide Fragmentation [PDF]
Chemistry – A European Journal, 2009 AbstractIn‐source oxidation: In‐source photocatalytic redox reactions for inducing peptide fragmentation are achieved on a TiO2‐derived target plate during laser desorption ionization mass spectrometry in the presence of samples and glucose acting as both an electron donor and a hole conductor (see scheme).magnified imageIn‐source photocatalytic redox ...
Hubert H. Girault +6 more
openaire +3 more sources
Selected Reaction Monitoring (SRM) frente a Western Blot [PDF]
, 2016 We propose the SRM technology as a complementary method to the Western Blot for the detection and quantification of proteins in a sample. The technique Western Blot has its own limitations: i) only a protein-of-choice is detected, ignoring any non ...
Heredia Ponce, Zaira Maria +1 more
core
Digestion of Protein in Premature and Term Infants. [PDF]
, 2012 Premature birth rates and premature infant morbidity remain discouragingly high. Improving nourishment for these infants is the key for accelerating their development and decreasing disease risk. Dietary protein is essential for growth and development of
Dallas, David C +3 more
core +2 more sources
Regulated Inositol‐Requiring Protein 1‐Dependent Decay as a Mechanism of Corin RNA and Protein Deficiency in Advanced Human Systolic Heart Failure [PDF]
, 2014 BACKGROUND: The compensatory actions of the endogenous natriuretic peptide system require adequate processing of natriuretic peptide pro‐hormones into biologically active, carboxyl‐terminal fragments.
Barton, P +5 more
core +1 more source