Cardiogenic programming of human pluripotent stem cells by dose-controlled activation of EOMES
Nature Communications, 2018 The T-box transcription factor eomesodermin (EOMES) acts both in endoderm specification as well as heart development, suggesting context-specific function.
Martin J. Pfeiffer +7 more
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Revised roles of ISL1 in a hES cell-based model of human heart chamber specification
eLife, 2018 The transcription factor ISL1 is thought to be key for conveying the multipotent and proliferative properties of cardiac precursor cells. Here, we investigate its function upon cardiac induction of human embryonic stem cells.
Roberto Quaranta +8 more
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Systematic optimization of human pluripotent stem cells media using Design of Experiments. [PDF]
, 2015 Human pluripotent stem cells (hPSC) are used to study the early stages of human development in vitro and, increasingly due to somatic cell reprogramming, cellular and molecular mechanisms of disease.
Chailangkarn, Thanathom +2 more
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Recreating Pluripotency? [PDF]
Cell Stem Cell, 2010 Two Matters Arising articles in this issue challenge the conclusions of a previous Cell Stem Cell paper that found extensive transcriptional differences between hESCs and hiPSCs. The original authors provide a response and set in motion a discussion in the field about appropriate methods for microarray data analysis.
Loh, Kyle M., Lim, Bing
openaire +2 more sources
Cardiac Subtype-Specific Modeling of Kv1.5 Ion Channel Deficiency Using Human Pluripotent Stem Cells
Frontiers in Physiology, 2017 The ultrarapid delayed rectifier K+ current (IKur), mediated by Kv1.5 channels, constitutes a key component of the atrial action potential. Functional mutations in the underlying KCNA5 gene have been shown to cause hereditary forms of atrial fibrillation
Maike Marczenke +12 more
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The RNA Helicase DDX6 Controls Cellular Plasticity by Modulating P-Body Homeostasis [PDF]
, 2019 Post-transcriptional mechanisms have the potential to influence complex changes in gene expression, yet their role in cell fate transitions remains largely unexplored.
Aigner, S. +21 more
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Adrenergic Stress Protection of Human iPS Cell-Derived Cardiomyocytes by Fast Kv7.1 Recycling
Frontiers in Physiology, 2017 The fight-or-flight response (FFR), a physiological acute stress reaction, involves positive chronotropic and inotropic effects on heart muscle cells mediated through β-adrenoceptor activation.
Ilaria Piccini +8 more
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Embryonic stem cell-specific signatures in cancer: insights into genomic regulatory networks and implications for medicine [PDF]
, 2011 Embryonic stem (ES) cells are of great interest as a model system for studying early developmental processes and because of their potential therapeutic applications in regenerative medicine.
Kim, Jonghwan, Orkin, Stuart
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Pluripotency in 3D: genome organization in pluripotent cells [PDF]
Current Opinion in Cell Biology, 2012 Cells face the challenge of storing two meters of DNA in the three-dimensional (3D) space of the nucleus that spans only a few microns. The nuclear organization that is required to overcome this challenge must allow for the accessibility of the gene regulatory machinery to the DNA and, in the case of embryonic stem cells (ESCs), for the transcriptional
Matthew, Denholtz, Kathrin, Plath
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NODAL Secures Pluripotency upon Embryonic Stem Cell Progression from the Ground State
Stem Cell Reports, 2017 Naive mouse embryonic stem cells (ESCs) can develop multiple fates, but the cellular and molecular processes that enable lineage competence are poorly characterized. Here, we investigated progression from the ESC ground state in defined culture.
Carla Mulas, Tüzer Kalkan, Austin Smith
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