Results 61 to 70 of about 333 (143)

Structural basis of intron selection by U2 snRNP in the presence of covalent inhibitors

, 2023
Intron selection during the formation of prespliceosomes is a critical event in pre-mRNA splicing. Chemical modulation of intron selection has emerged as a route for cancer therapy.
Gee, P, Liu, X, Nguyen, T-V, Ghosh, AK, Agrawal, A, Larsen, NA, Pena, V, Cook, A, Jurica, M, Cretu, C   +9 more
core   +1 more source

Targeting the Spliceosomal Protein USP39 Through Allosteric Ligands and PROTAC‐Induced Degradation

Angewandte Chemie International Edition, Volume 65, Issue 5, 28 January 2026.
Proteolysis‐targeting chimeras (PROTACs) enable degradation of proteins previously considered undruggable by harnessing the cellular ubiquitin–proteasome system. In this study, Schäfer et al. identify thiazole‐based small molecules that allosterically bind the zinc finger domain of ubiquitin‐specific protease 39 (USP39), a non‐enzymatic scaffold ...
Daniel Schäfer, Cristian Prieto‐Garcia, Jianhui Wang, Marcel Heinz, Vigor Matkovic, Pavel Kielkowski, Sebastian Hasselbeck, Varun Jayeshkumar Shah, Stefan Knapp, Gerhard Hummer, Ivan Dikic, Xinlai Cheng   +11 more
wiley   +1 more source

Long non‐coding RNA lncAPAT promotes atherosclerotic plaque instability by targeting ribosomal protein L22

Clinical and Translational Medicine, Volume 16, Issue 1, January 2026.
LncAPAT interacted with the promoter region of RPL22 to inhibit RPL22 transcriptional activity and increase the expression of MCP‐1, thereby contributing to plaque instability. Abstract Background Long non‐coding RNAs (lncRNAs) regulate macrophage inflammation and atherosclerotic plaque stability, but mechanisms need comprehensive investigations ...
Rongxia Li, Qiyue Zhang, Yu Chen, Shuting Wang, Shuang Han, Adalaiti Kamili, Yixuan Zhong, Shujun Yang, Weili Zhang   +8 more
wiley   +1 more source

U1 and U2 small nuclear RNAs are present in nuclear speckles [PDF]

, 1992
The localization of U1 and U2 small nuclear RNAs (snRNAs) has been examined by in situ hybridization using 2'-O-alkyl oligonucleotide probes. We have found that these snRNAs, which are essential for pre-mRNA splicing, localize in a speckled distribution,
Spector, D. L., Huang, S.
core  

Spliceosome assembly involves the binding and release of U4 small nuclear ribonucleoprotein.

, 1988
Splicing complexes that form a rabbit beta-globin precursor mRNA (pre-mRNA) have been analyzed for their small nuclear RNA (snRNA) content by both affinity chromatography and specific probe hybridization of replicas of native electrophoretic gels.
Lamond, A. I.; id_orcid, Grabowski, P. J., Sharp, P. A., Konarska, M. M.   +3 more
core   +1 more source

Multidimensional OMICs reveal ARID1A orchestrated control of DNA damage, splicing, and cell cycle in normal‐like and malignant urothelial cells

Molecular Oncology, Volume 19, Issue 12, Page 3784-3805, December 2025.
Loss of the frequently mutated chromatin remodeler ARID1A, a subunit of the SWI/SNF cBAF complex, results in less open chromatin, alternative splicing, and the failure to stop cells from progressing through the cell cycle after DNA damage in bladder (cancer) cells. Created in BioRender. Epigenetic regulators, such as the SWI/SNF complex, with important
Rebecca M. Schlösser, Florian Krumbach, Eyleen Corrales, Geoffroy Andrieux, Christian Preisinger, Franziska Liss, Alexandra Golzmann, Melanie Boerries, Kerstin Becker, Ruth Knüchel, Stefan Garczyk, Bernhard Lüscher   +11 more
wiley   +1 more source

Isoforms of U1-70k control subunit dynamics in the human spliceosomal U1 snRNP

, 2009
Most human protein-encoding genes contain multiple exons that are spliced together, frequently in alternative arrangements, by the spliceosome. It is established that U1 snRNP is an essential component of the spliceosome, in human consisting of RNA and ...
Nina Morgner, Hernández Helena, Makarov Evgeny M., H. Hernández (7607219), Yutaka Muto, D. P. Krummel (7607234), Helena Hernández, Carol V Robinson, O. V. Makarova (7607222), Robinson, CV, Krummel, DP, Daniel Pomeranz Krummel, Hernandez, H, Evgeny M Makarov, Makarova, OV, Y. Muto (7607231), Olga V Makarova, Robinson Carol V., E. M. Makarov (7607225), N. Morgner (7607228), Makarov, EM, Robinson, Carol, Makarova Olga V., Muto Yutaka, Krummel Daniel Pomeranz, C. V. Robinson (7607237), Morgner, N, Morgner Nina, Muto, Y   +28 more
core   +1 more source

Molecular architecture of the human 17S U2 snRNP

, 2020
The U2 small nuclear ribonucleoprotein (snRNP) has an essential role in the selection of the precursor mRNA branch-site adenosine, the nucleophile for the first step of splicing1. Stable addition of U2 during early spliceosome formation requires the DEAD-
Hartmuth, K., Bertram, K., Stark, H., Will, Cindy L., Urlaub, Henning, Agafonov, D., Stark, Holger, Zhang, Zhenwei, Zhang, Z., Urlaub, H., Agafonov, Dmitry E., Bertram, Karl, Hartmuth, Klaus, Will, C., Kastner, B., Dybkov, Olexandr, Hofele, Romina, Kastner, Berthold, Dybkov, O., Lührmann, R., Hofele, R., Lührmann, Reinhard   +21 more
core   +1 more source

S-allylmercaptocysteine inhibits TLR4-mediated inflammation through enhanced formation of inhibitory MyD88 splice variant in mammary epithelial cells

Scientific Reports
Mastitis is an inflammatory disease affecting mammary tissues caused by bacterial infection that negatively affects milk quality and quantity. S-Allylmercaptocysteine (SAMC), a sulfur compound in aged garlic extract (AGE), suppresses lipopolysaccharide ...
Miyuki Takashima, Masahiro Kurita, Haruhi Terai, Feng-Qi Zhao, Jun-ichiro Suzuki   +4 more
doaj   +1 more source

The Influence of Cell Isolation and Culturing on Natriuretic Peptide Receptors in Aortic Vascular Smooth Muscle Cells

Cells
Vascular smooth muscle cell (SMC) relaxation by guanylyl cyclases (GCs) and cGMP is mediated by NO and its receptor soluble GC (sGC) or natriuretic peptides (NPs) ANP/BNP and CNP with the receptors GC-A and GC-B, respectively.
Christine Rager, Tobias Klöpper, Sabine Tasch, Michael Raymond Whittaker, Betty Exintaris, Andrea Mietens, Ralf Middendorff   +6 more
doaj   +1 more source