Results 71 to 80 of about 333 (143)

Functioning of the Drosophila integral U1/U2 protein Snf independent of U1 and U2 small nuclear ribonucleoprotein particles is revealed by snf ⁺ gene dose effects [PDF]

Proceedings of the National Academy of Sciences, 1999
Snf, encoded by sans fille , is the Drosophila homolog of mammalian U1A and U2B′′ and is an integral component of U1 and U2 small nuclear ribonucleoprotein particles (snRNPs). Surprisingly, changes in the level of this housekeeping protein can specifically affect autoregulatory activity of the RNA-binding protein ...
T W, Cline, D Z, Rudner, D A, Barbash, M, Bell, R, Vutien   +4 more
openaire   +2 more sources

Genome‐wide CRISPR screen reveals an uncharacterized spliceosome regulator as new candidate immunotherapy target

iMeta, Volume 4, Issue 6, December 2025.
This study identifies the uncharacterized gene C9ORF50 as a novel regulator of immune evasion. Functioning as an intrinsically disordered protein, C9ORF50 drives liquid‐liquid phase separation to facilitate spliceosome assembly and maintain RNA splicing fidelity.
Tong Shao, Chuanyang Liu, Jingyu Kuang, Sisi Xie, Ying Qu, Yingying Li, Lulu Zhang, Fangzhou Liu, Yanhua Qi, Tao Hou, Ming Li, Sujuan Zhang, Yu Liu, Zhixiang Yuan, Jiali Liu, Yanming Hu, Jingyang Wang, Chenghu Song, Shaowei Zhang, Lingyun Zhu, Jianzhong Shao, Aifu Lin, Wenjun Mao, Guangchuan Wang, Lvyun Zhu   +24 more
wiley   +1 more source

C16orf57, a gene mutated in poikiloderma with neutropenia, encodes a putative phosphodiesterase responsible for the U6 snRNA 3′ end modification. [PDF]

, 2012
C16orf57 encodes a human protein of unknown function, and mutations in the gene occur in poikiloderma with neutropenia (PN), which is a rare, autosomal recessive disease.
Krwawicz, Joanna, Lazniewski, Michal, Mroczek, Seweryn, Kutner, Jan, Kucinski, Iwo, Ginalski, Krzysztof, Dziembowski, Andrzej   +6 more
core   +1 more source

A Block in Mammalian Splicing Occurring after Formation of Large Complexes Containing U1, U2, U4, U5, and U6 Small Nuclear Ribonucleoproteins [PDF]

Molecular and Cellular Biology, 1989
The assembly of mammalian pre-mRNAs into large 50S to 60S complexes, or spliceosomes, containing small nuclear ribonucleoproteins (snRNPs) leads to the production of splicing intermediates, 5' exon and lariat-3' exon, and the subsequent production of spliced products.
C H, Agris, M E, Nemeroff, R M, Krug
openaire   +2 more sources

Alternative Splicing: Molecular Mechanisms, Biological Functions, Diseases, and Potential Therapeutic Targets

MedComm, Volume 6, Issue 12, December 2025.
Alternative splicing (AS) expands proteomic diversity and functional complexity in eukaryotes, regulated by spliceosomal components, RNA elements, and epigenetic modifications. Dysregulated AS contributes to diseases, including cancer, neurodegenerative disorders, and cardiovascular conditions, among others. Therapeutic interventions, such as antisense
Zhi‐Min Zhu, Xiao‐Mei Wu, Yan Hu, Xiao‐Lan Bian, Ya‐Qin Wang, Qiong‐Ni Zhu   +5 more
wiley   +1 more source

An ATP-independent U2 small nuclear ribonucleoprotein particle/precursor mRNA complex requires both splice sites and the polypyrimidine tract. [PDF]

Proceedings of the National Academy of Sciences, 1992
A complex is formed upon incubation of a precursor mRNA (pre-mRNA) with HeLA cell nuclear extract in the absence of added ATP (-ATP complex). Pre-mRNAs with mutations in the 5' splice site, the 3' splice site, or the polypyrimidine tract did not form this complex. Once formed, the -ATP complex was stable to competition by excess pre-mRNA.
S F, Jamison, M A, Garcia-Blanco
openaire   +2 more sources

Mining Alzheimer's Interactomes, Macromolecular Complexes and Pathways for Drug Discovery

PROTEOMICS, Volume 25, Issue 21-22, Page 102-150, November 2025.
ABSTRACT Alzheimer's disease (AD) is a progressive neurodegenerative disorder that leads to dementia. Many cases are diagnosed annually and there is no currently available cure. Understanding the underlying disease biology of AD through the study of molecular networks, particularly by mapping clinical variants to tissue‐specific interactomes and ...
Kalpana Panneerselvam, Krishna Kumar Tiwari, Luana Licata, Simona Panni, Sylvie Ricard‐Blum, Sucharitha Balu, Susie Huget, Juan Jose Medina Reyes, Eliot Ragueneau, Livia Perfetto, Birgit Meldal, Sandra Orchard, Henning Hermjakob   +12 more
wiley   +1 more source

ATP requirement for Prp5p function is determined by Cus2p and the structure of U2 small nuclear RNA [PDF]

, 2003
Stable addition of U2 small nuclear ribonucleoprotein (snRNP) to form the prespliceosome is the first ATP-dependent step in splicing, and it requires the DEXD/H box ATPase Prp5p.
Voeltz, Gia K., Ares, Manuel, Jr., Barta, Imre, Abelson, John, Perriman, Rhonda   +4 more
core  

Additional file 5 of Phylogenetic analysis and stress response of the plant U2 small nuclear ribonucleoprotein B″ gene family

, 2022
Additional file 5: Table S4. Expression of U2B″ in rice under different stresses.
Gao, Cong, Lu, Shuai, Zhou, Rong, Ding, Junjie, Fan, Jialiang, Han, Binying, Chen, Moxian, Wang, Baohua, Cao, Yunying   +8 more
openaire   +1 more source

The 5' splice site consensus RNA oligonucleotide induces assembly of U2/U4/U5/U6 small nuclear ribonucleoprotein complexes. [PDF]

Proceedings of the National Academy of Sciences, 1992
A short RNA oligonucleotide comprising the 5' splice site consensus sequence (5'SS RNA oligo) efficiently inhibits splicing of mRNA precursors in HeLa cell nuclear extracts. Addition of 5'SS RNA oligo inhibits early, but not late, steps in the splicing reaction, affecting the process of spliceosome assembly. In the presence of 5'SS RNA oligo a majority
K B, Hall, M M, Konarska
openaire   +2 more sources