Malleable ribonucleoprotein machine: protein intrinsic disorder in the Saccharomyces cerevisiae spliceosome [PDF]
PeerJ, 2013 Recent studies revealed that a significant fraction of any given proteome is presented by proteins that do not have unique 3D structures as a whole or in significant parts.
Maria de Lourdes Coelho Ribeiro +9 more
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Purification of the yeast U4/U6⋅U5 small nuclear ribonucleoprotein particle and identification of its proteins [PDF]
Proceedings of the National Academy of Sciences, 1999 The yeast U4/U6⋅U5 pre-mRNA splicing small nuclear ribonucleoprotein (snRNP) is a 25S small nuclear ribonucleoprotein particle similar in size, composition, and morphology to its counterpart in human cells. The yeast U4/U6⋅U5 snRNP complex has been purified to near homogeneity by affinity chromatography and preparative glycerol gradient sedimentation ...
Stevens, Scott W., Abelson, John
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Identification of a 35S U4/U6.U5 tri-small nuclear ribonucleoprotein (tri-snRNP) complex intermediate in spliceosome assembly [PDF]
Journal of Biological Chemistry, 2017 The de novo assembly and post-splicing reassembly of the U4/U6.U5 tri-snRNP remain to be investigated. We report here that ZIP, a protein containing a CCCH-type zinc finger and a G-patch domain, as characterized by us previously, regulates pre-mRNA splicing independent of RNA binding.
Zhe, Chen +14 more
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The 5' splice site consensus RNA oligonucleotide induces assembly of U2/U4/U5/U6 small nuclear ribonucleoprotein complexes. [PDF]
Proceedings of the National Academy of Sciences, 1992 A short RNA oligonucleotide comprising the 5' splice site consensus sequence (5'SS RNA oligo) efficiently inhibits splicing of mRNA precursors in HeLa cell nuclear extracts. Addition of 5'SS RNA oligo inhibits early, but not late, steps in the splicing reaction, affecting the process of spliceosome assembly. In the presence of 5'SS RNA oligo a majority
K B, Hall, M M, Konarska
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Structural basis for dual roles of Aar2p in U5 snRNP assembly [PDF]
, 2013 Yeast U5 small nuclear ribonucleoprotein particle (snRNP) is assembled via a cytoplasmic precursor that contains the U5-specific Prp8 protein but lacks the U5-specific Brr2 helicase.
Beggs, J.D. +8 more
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Cloning and molecular characterization of Trypanosoma cruzi U2, U4, U5, and U6 small nuclear RNAs
Memorias do Instituto Oswaldo Cruz, 2007 Small nuclear RNAs (snRNAs) are important factors in the functioning of eukaryotic cells that form several small complexes with proteins; these ribonucleoprotein particles (U snRNPs) have an essential role in the pre-mRNA processing, particularly in ...
DL Ambrósio, MTA Silva, RMB Cicarelli
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PRP4: a protein of the yeast U4/U6 small nuclear ribonucleoprotein particle [PDF]
, 1989 The Saccharomyces cerevisiae prp mutants (prp2 through prp11) are known to be defective in pre-mRNA splicing at nonpermissive temperatures. We have sequenced the PRP4 gene and shown that it encodes a 52-kilodalton protein.
Abelson, John N., Banroques, Josette
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Anti-U5 Small Nuclear Ribonucleoprotein (snRNP) Antibodies: A Rare Anti-U snRNP Specificity
Clinical Immunology and Immunopathology, 1996 We surveyed for autoantibodies to the U small nuclear ribonucleoproteins (snRNPs) in sera from 1171 patients with various connective tissue diseases by immunoprecipitation assay. We found serum, termed LaJ, which principally recognized the U5 snRNP from one patient with systemic sclerosis-polymyositis overlap syndrome. Anti-LaJ serum immunoprecipitated
Y, Okano +9 more
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A U5 Small Nuclear Ribonucleoprotein Particle Protein Involved Only in the Second Step of Pre-mRNA Splicing in Saccharomyces cerevisiae [PDF]
Molecular and Cellular Biology, 1993 The PRP18 gene, which had been identified in a screen for pre-mRNA splicing mutants in Saccharomyces cerevisiae, has been cloned and sequenced. Yeast strains bearing only a disrupted copy of PRP18 are temperature sensitive for growth; even at a low temperature, they grow extremely slowly and do not splice pre-mRNA efficiently.
Horowitz, David S., Abelson, John
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Evidence for a nuclear compartment of transcription and splicing located at chromosome domain boundaries [PDF]
, 1993 The nuclear topography of splicing snRNPs, mRNA transcripts and chromosome domains in various mammalian cell types are described. The visualization of splicing snRNPs, defined by the Sm antigen, and coiled bodies, revealed distinctly different ...
A Monneron +64 more
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