Knocking Down Snrnp200 Initiates Demorphogenesis of Rod Photoreceptors in Zebrafish
Journal of Ophthalmology, 2015 Purpose. The small nuclear ribonucleoprotein 200 kDa (SNRNP200) gene is a fundamental component for precursor message RNA (pre-mRNA) splicing and has been implicated in the etiology of autosomal dominant retinitis pigmentosa (adRP).
Yuan Liu +6 more
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Alternative Spliceosome Assembly Pathways Revealed by Single-Molecule Fluorescence Microscopy
Cell Reports, 2013 Removal of introns from nascent transcripts (pre-mRNAs) by the spliceosome is an essential step in eukaryotic gene expression. Previous studies have suggested that the earliest steps in spliceosome assembly in yeast are highly ordered and the stable ...
Inna Shcherbakova +7 more
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A subset of nuclear receptor coregulators act as coupling proteins during synthesis and maturation of RNA transcripts [PDF]
, 2005 Gene expression is a multistep process starting in the cell nucleus with the synthesis of the primary transcripts that undergo several modifications (including capping, splicing, and polyadenylation) leading to the export of the mature mRNAs into the ...
Auboeuf, Didier +5 more
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The basic domain of Rev from human immunodeficiency virus type 1 specifically blocks the entry of U4/U6.U5 small nuclear ribonucleoprotein in spliceosome assembly [PDF]
Journal of Virology, 1993 Human immunodeficiency virus type 1 (HIV-1) encodes a regulatory protein, Rev, which is required for cytoplasmic expression of incompletely spliced viral mRNA. Rev binds to a cis-acting Rev-responsive element (RRE) located within the env region of HIV-1. It has previously been shown that a 17-amino-acid peptide, corresponding to the basic domain of Rev,
J, Kjems, P A, Sharp
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A human protein required for the second step of pre-mRNA splicing is functionally related to a yeast splicing factor [PDF]
, 1997 We have identified a human splicing factor required for the second step of pre-mRNA splicing. This new protein, hPrp18, is 30% identical to the yeast splicing factor Prp18.
Horowitz, D. S., Krainer, A. R.
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Nucleic Acids Research, 1989 As demonstrated by RNase T1 protection assays at 0 degrees C without ATP, U1 and U5 snRNPs purified by isopycnic centrifugation in cesium chloride bind to the 5' and 3' splice sites of human beta-globin pre-mRNA, respectively. We also devised a saturation-complementation assay and have found that this purified U5 snRNP, unlike U1, successfully competes
J, Tazi +7 more
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eLife, 2018 Transcription-translation feedback loops that comprise eukaryotic circadian clocks rely upon temporal delays that separate the phase of active transcription of clock genes, such as Drosophila period (per) and timeless (tim), from negative feedback by the
Iryna Shakhmantsir +3 more
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Schizosaccharomyces U6 genes have a sequence within their introns that matches the B box consensus of tRNA internal promoters [PDF]
, 1990 The gene for the U6 small nuclear RNA (snRNA) in the fission yeast Schizosaccharomyces pombe is interrupted by an intron whose structure is similar to those found in messenger RNA precursors (pre-mRNAs) (1).
Frendewey, David +3 more
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Splicing of the U6 RNA precursor is impaired in fission yeast pre-mRNA splicing mutants [PDF]
, 1989 U6 RNA is a member of a class of small abundant stable nuclear RNAs that are essential for splicing. In all species examined so far, the U6 RNA is a RNA polymerase III transcript.
Potashkin, J., Frendewey, D.
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Differential distribution of factors involved in pre-mRNA processing in the yeast cell nucleus [PDF]
, 1990 The yeast cell nucleus has previously been shown to be divided into two regions by a variety of microscopic approaches. We used antibodies specific for the 2,2,7-trimethylguanosine cap structure of small nuclear ribonucleic acids (snRNAs) and for a ...
Potashkin, J. A. +2 more
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