Cloning and molecular characterization of Trypanosoma cruzi U2, U4, U5, and U6 small nuclear RNAs
Memorias do Instituto Oswaldo Cruz, 2007 Small nuclear RNAs (snRNAs) are important factors in the functioning of eukaryotic cells that form several small complexes with proteins; these ribonucleoprotein particles (U snRNPs) have an essential role in the pre-mRNA processing, particularly in ...
DL Ambrósio, MTA Silva, RMB Cicarelli
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Association of U2, U4, U5, and U6 small nuclear ribonucleoproteins in a spliceosome-type complex in absence of precursor RNA. [PDF]
Proceedings of the National Academy of Sciences, 1988 Small nuclear ribonucleoprotein particles (snRNPs) associate to form multi-snRNP complexes during splicing of mRNA precursors. A vast majority of the three snRNPs U4, U5, and U6 are present in a nuclear extract in a single complex, while U1 and U2 snRNPs exist as separate particles.
M M, Konarska, P A, Sharp
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A Block in Mammalian Splicing Occurring after Formation of Large Complexes Containing U1, U2, U4, U5, and U6 Small Nuclear Ribonucleoproteins [PDF]
Molecular and Cellular Biology, 1989 The assembly of mammalian pre-mRNAs into large 50S to 60S complexes, or spliceosomes, containing small nuclear ribonucleoproteins (snRNPs) leads to the production of splicing intermediates, 5' exon and lariat-3' exon, and the subsequent production of spliced products.
C H, Agris, M E, Nemeroff, R M, Krug
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The 5' splice site consensus RNA oligonucleotide induces assembly of U2/U4/U5/U6 small nuclear ribonucleoprotein complexes. [PDF]
Proceedings of the National Academy of Sciences, 1992 A short RNA oligonucleotide comprising the 5' splice site consensus sequence (5'SS RNA oligo) efficiently inhibits splicing of mRNA precursors in HeLa cell nuclear extracts. Addition of 5'SS RNA oligo inhibits early, but not late, steps in the splicing reaction, affecting the process of spliceosome assembly. In the presence of 5'SS RNA oligo a majority
K B, Hall, M M, Konarska
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Dual RNA Processing Roles of Pat1b via Cytoplasmic Lsm1-7 and Nuclear Lsm2-8 Complexes
Cell Reports, 2017 Summary: Pat1 RNA-binding proteins, enriched in processing bodies (P bodies), are key players in cytoplasmic 5′ to 3′ mRNA decay, activating decapping of mRNA in complex with the Lsm1-7 heptamer.
Caroline Vindry +10 more
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Anti-U5 Small Nuclear Ribonucleoprotein (snRNP) Antibodies: A Rare Anti-U snRNP Specificity
Clinical Immunology and Immunopathology, 1996 We surveyed for autoantibodies to the U small nuclear ribonucleoproteins (snRNPs) in sera from 1171 patients with various connective tissue diseases by immunoprecipitation assay. We found serum, termed LaJ, which principally recognized the U5 snRNP from one patient with systemic sclerosis-polymyositis overlap syndrome. Anti-LaJ serum immunoprecipitated
Y, Okano +9 more
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Affinity purification of spliceosomes reveals that the precursor RNA processing protein PRP8, a protein in the U5 small nuclear ribonucleoprotein particle, is a component of yeast spliceosomes. [PDF]
Proceedings of the National Academy of Sciences, 1990 Nuclear pre-mRNA splicing in Saccharomyces cerevisiae, as in higher eukaryotes, occurs in large RNA-protein complexes called spliceosomes. The small nuclear RNA components, U1, U2, U4, U5, and U6, have been extensively studied; however, very little is known about the protein components of yeast spliceosomes. Here we use antibodies against the precursor
E, Whittaker, M, Lossky, J D, Beggs
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Knocking Down Snrnp200 Initiates Demorphogenesis of Rod Photoreceptors in Zebrafish
Journal of Ophthalmology, 2015 Purpose. The small nuclear ribonucleoprotein 200 kDa (SNRNP200) gene is a fundamental component for precursor message RNA (pre-mRNA) splicing and has been implicated in the etiology of autosomal dominant retinitis pigmentosa (adRP).
Yuan Liu +6 more
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Alternative Spliceosome Assembly Pathways Revealed by Single-Molecule Fluorescence Microscopy
Cell Reports, 2013 Removal of introns from nascent transcripts (pre-mRNAs) by the spliceosome is an essential step in eukaryotic gene expression. Previous studies have suggested that the earliest steps in spliceosome assembly in yeast are highly ordered and the stable ...
Inna Shcherbakova +7 more
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The basic domain of Rev from human immunodeficiency virus type 1 specifically blocks the entry of U4/U6.U5 small nuclear ribonucleoprotein in spliceosome assembly [PDF]
Journal of Virology, 1993 Human immunodeficiency virus type 1 (HIV-1) encodes a regulatory protein, Rev, which is required for cytoplasmic expression of incompletely spliced viral mRNA. Rev binds to a cis-acting Rev-responsive element (RRE) located within the env region of HIV-1. It has previously been shown that a 17-amino-acid peptide, corresponding to the basic domain of Rev,
J, Kjems, P A, Sharp
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