Results 21 to 30 of about 80 (78)

Purified U5 small nuclear ribonucleoprotein can relieve the inhibition of spliceosome assemblyand spiking by snRNP-free nuclear proteins

Nucleic Acids Research, 1989
As demonstrated by RNase T1 protection assays at 0 degrees C without ATP, U1 and U5 snRNPs purified by isopycnic centrifugation in cesium chloride bind to the 5' and 3' splice sites of human beta-globin pre-mRNA, respectively. We also devised a saturation-complementation assay and have found that this purified U5 snRNP, unlike U1, successfully competes
J, Tazi, J, Temsamani, C, Alibert, W, Rhead, S, Khellil, G, Cathala, C, Brunel, P, Jeanteur   +7 more
openaire   +3 more sources

Spliceosome factors target timeless (tim) mRNA to control clock protein accumulation and circadian behavior in Drosophila

eLife, 2018
Transcription-translation feedback loops that comprise eukaryotic circadian clocks rely upon temporal delays that separate the phase of active transcription of clock genes, such as Drosophila period (per) and timeless (tim), from negative feedback by the
Iryna Shakhmantsir, Soumyashant Nayak, Gregory R Grant, Amita Sehgal   +3 more
doaj   +1 more source

Biochemical and genetic analyses of the U5, U6, and U4/U6 x U5 small nuclear ribonucleoproteins from Saccharomyces cerevisiae.

RNA (New York, N.Y.), 2001
Copyright © 2001 by RNA Society. Received July 3, 2001; returned for revision August 14, 2001; revised manuscript received August 27, 2001. We gratefully acknowledge the generous gift of strains and hybridoma cells from Christine Guthrie. We are also grateful for helpful conversations with the members of the Abelson, Guthrie, and Lührmann laboratories ...
Stevens, Scott W., Barta, Imre, Ge, Helen Y., Moore, Roger E., Young, Mary K., Lee, Terry D., Abelson, John   +6 more
openaire   +3 more sources

Addressing the tissue specificity of U5 snRNP spliceosomopathies

Frontiers in Cell and Developmental Biology
Precursor mRNA (pre-mRNA) must undergo splicing to remove intron sequences and join exons. This splicing process is catalysed by an RNA/protein complex called the spliceosome.
Rahmat Azhari Kemal, Rahmat Azhari Kemal, Raymond T. O’Keefe   +2 more
doaj   +1 more source

Targeting Human Immunodeficiency Virus Integrase Beyond the Active Site: The Discovery and Development of Allosteric Integrase Inhibitors

ChemMedChem, Volume 21, Issue 16, 27 August 2026.
Human immunodeficiency virus (HIV) allosteric integrase inhibitors (ALLINIs) offer a vital alternative to standard therapies by targeting noncatalytic sites. By inducing aberrant integrase multimerization, they disrupt viral maturation. This review explores their medicinal chemistry evolution, detailing structural insights, structure–activity ...
Francesco Saccoliti, Elisa Patacchini, Emanuele Cara, Laura Zarbo, Antonella Messore, Aurora Albano, Giuseppe Ruggieri, Valentina Noemi Madia, Luigi Scipione, Roberta Costi, Roberto Di Santo   +10 more
wiley   +1 more source

A label free chemoproteomic-based platform to disclose cannabidiol molecular mechanism of action on chronic myelogenous leukemia cancer cells

Heliyon
The discovery of the interactome of cannabidiol (CBD), a non-psychoactive cannabinoid from Cannabis sativa L., has been here performed on chronic myelogenous leukemia cancer cells, using an optimized chemo-proteomic stage, which links Drug Affinity ...
Sara Ceccacci, Lorenzo Corsi, Lucio Spinelli, Clarissa Caroli, Matilde Marani, Lisa Anceschi, Matteo Mozzicafreddo, Federica Pellati, Maria Chiara Monti   +8 more
doaj   +1 more source

Caspase‐mediated cleavage events hidden by secondary proteolysis during apoptosis

Protein Science, Volume 35, Issue 8, August 2026.
Abstract Caspases are cysteine proteases that cleave specific proteins to control a range of cellular processes including cell death, inflammation, and differentiation. Proteomic approaches, like N‐terminomics, have been central to identifying both cleaved proteins and where they are cleaved.
Fatemeh Fotouhi, Erik Gomez‐Cardona, Jainilkumar Patel, Olivier Julien, Howard Fearnhead   +4 more
wiley   +1 more source

Electron microscopy of small nuclear ribonucleoprotein (snRNP) particles U2 and U5: evidence for a common structure-determining principle in the major U snRNP family.

Proceedings of the National Academy of Sciences, 1990
We have studied by electron microscopy the structures of native small nuclear ribonucleoprotein (snRNP) particles U2 and U5 from HeLa cells. The structure of native U2 snRNP is characterized by a main body 8 nm in diameter with one additional domain about 4 nm long and 6 nm wide.
B, Kastner, M, Bach, R, Lührmann
openaire   +3 more sources

Interaction of Newcastle disease virus V protein with EFTUD2 modulates MDA5 pathway to suppress viral replication

Poultry Science
Elongation factor Tu GTP-binding domain-containing protein 2 (EFTUD2), a core component of U5 small nuclear ribonucleoprotein particles (snRNPs), recently emerged as a novel innate immune regulator.
Yin Han, Fan Zhang, Ziqing Zhou, Yufang Huang, Ruiai Chen   +4 more
doaj   +1 more source

Pathogen‐Induced Shifts in Alternative Splicing Shaping Plant Defense

Food and Energy Security, Volume 15, Issue 3, May/June 2026.
ABSTRACT Plant immune responses comprise a highly coordinated and dynamic defense network that enables the recognition and containment of diverse microbial pathogens. Alternative splicing (AS), a critical post‐transcriptional regulatory mechanism that generates multiple mRNA isoforms from a single pre‐mRNA precursor, substantially enhances proteomic ...
Zijing Li, Mojing Zhang, Muhammad Aamir Khan, Muhammad Ali   +3 more
wiley   +1 more source