Results 121 to 130 of about 2,250 (151)

RT-QuIC as ultrasensitive method for prion detection

Cell and Tissue Research, 2022
Real-time quaking-induced conversion (RT-QuIC) is a cell-free abnormal form of prion protein (PrPSc) amplification method using recombinant prion protein from Escherichia coli that can measure prion seeding activity in samples with high sensitivity. The advantages of this method are that it is much more sensitive than Western blotting, which is usually
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Nanoparticle-Enhanced RT-QuIC (Nano-QuIC) Diagnostic Assay for Misfolded Proteins

Nano Letters, 2023
Misfolded proteins associated with various neurodegenerative diseases often accumulate in tissues or circulate in biological fluids years before the clinical onset, thus representing ideal diagnostic targets. Real-time quaking-induced conversion (RT-QuIC), a protein-based seeded-amplification assay, holds great potential for early disease detection ...
Peter R Christenson, Manci Li, Gage Rowden   +2 more
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α-Synuclein Seeding Assay Using RT-QuIC

2021
Synucleinopathies are neurodegenerative diseases that are associated with the misfolding and aggregation of α-synuclein (αSyn). They include Parkinson's disease, dementia with Lewy bodies, and multiple system atrophy. In each disease, it has been proposed that aggregates of αSyn represent different conformational strains of αSyn, leading to self ...
Ayami, Okuzumi, Taku, Hatano, Takeshi, Fukuhara, Shinichi, Ueno, Nobuyuki, Nukina, Yuzuru, Imai, Nobutaka, Hattori   +6 more
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Alpha‐Synuclein RT‐QuIC in Idiopathic Normal Pressure Hydrocephalus

Annals of Neurology, 2022
This study quantified the occurrence of an underlying synucleinopathy in 50 patients with idiopathic normal pressure hydrocephalus by means of real‐time quaking‐induced conversion, a highly sensitive and specific technique capable of detecting and amplifying misfolded aggregated forms of α‐synuclein in the cerebrospinal fluid.
Alfonso Fasano, Ivan Martinez‐Valbuena, Paula Azevedo, Carolina Candeias da Silva, Musleh Algarni, Anna Vasilevskaya, Chloe Anastassiadis, Foad Taghdiri, Paul Kongkham, Ivan Radovanovic, Gelareh Zadeh, Anthony E. Lang, David F. Tang‐Wai, Gabor G. Kovacs, Maria Carmela Tartaglia   +14 more
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RT-QuIC Assays for Prion Disease Detection and Diagnostics

2017
In coping with prion diseases, it is important to have tests that are practical enough for routine applications in medicine, agriculture, wildlife biology, and research, yet sensitive enough to detect minimal amounts of infectivity. Real-time quaking-induced conversion (RT-QuIC) assays have evolved to the point where they fulfill these criteria in ...
Christina D, Orrù, Bradley R, Groveman, Andrew G, Hughson, Matteo, Manca, Lynne D, Raymond, Gregory J, Raymond, Katrina J, Campbell, Kelsie J, Anson, Allison, Kraus, Byron, Caughey   +9 more
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Seed amplification and RT-QuIC assays to investigate protein seed structures and strains

Cell and Tissue Research, 2022
The accumulation of misfolded proteins as amyloid fibrils in the brain is characteristic of most neurodegenerative disorders. These misfolded proteins are capable of self-amplifying through protein seeding mechanisms, leading to accumulation in the host. First shown for PrP prions and prion diseases, it is now recognized that self-propagating misfolded
Heidi G. Standke, Allison Kraus
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Tracking Prions by RT-QuIC: An Update

Prion seed amplification assays, such as real-time quaking-induced conversion (RT-QuIC), are providing ultrasensitive, specific, and increasingly practical means of detecting prions and diagnosing prion diseases in humans and other animals. Widespread efforts have greatly increased the breadth of RT-QuIC applications for research, surveillance, and ...
Christina D, Orrù, Bradley R, Groveman, Byron, Caughey   +2 more
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A systematic review comparing the diagnostic value of 14-3-3 protein in the cerebrospinal fluid, RT-QuIC and RT-QuIC on nasal brushing in sporadic Creutzfeldt–Jakob disease

Acta Neurologica Belgica, 2018
Sporadic Creutzfeldt-Jakob disease (sCJD) is a human prion disease that is a relatively common differential diagnosis in dementia patients. Therefore it needs a good diagnostic tool. Brain autopsy is the golden standard for the diagnosis of CJD; however, a less invasive technique is 14-3-3 protein measurement in the cerebrospinal fluid (CSF).
Olivia Behaeghe, Elias Mangelschots, Bart De Vil, Patrick Cras   +3 more
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Detection and Diagnosis of Prion Diseases Using RT-QuIC: An Update

2017
Until recently, it has been difficult to detect all infectious levels of prions and diagnose prion diseases in living humans and other animals. Real-time quaking-induced conversion (RT-QuIC), an ultrasensitive test based on amplification of the amyloid seeding activity of prions, is now achieving the sensitivity and practicality required for routine ...
Caughey, Byron, Orru, Christina D., Groveman, Bradley R., Bongianni, Matilde, Hughson, Andrew G., Raymond, Lynne D., Manca, Matteo, Kraus, Allison, Raymond, Gregory J., Fiorini, Michele, Pocchiari, Maurizio, Zanusso, Gianluigi   +11 more
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Advancing Prion Diagnostics: RT-QuIC Applications in Peripheral Tissues

Prion diseases (PrDs) are fatal neurodegenerative disorders characterized by the accumulation of misfolded prion protein (PrPSc) in the central nervous system (CNS). This pathological isoform of the cellular prion protein drives disease pathogenesis through its unique ability to propagate itself via a template-directed misfolding mechanism.
Bacınoglu, Merve Begum, Bufano, Giuseppe, Cazzaniga, Federico Angelo, Zanusso, Gianluigi, Legname, Giuseppe, Moda, Fabio   +5 more
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