Results 31 to 40 of about 11,168 (173)

Comparative analysis of CO2 reduction by soluble Escherichia coli formate dehydrogenase H and its selenocysteine-to-cysteine substitution variant

Journal of CO2 Utilization, 2023
Metal-dependent formate dehydrogenases (Me-FDHs) are highly active CO2-reducing enzymes operating at low redox potentials and employ either molybdenum or tungsten to reduce the bound substrate.
Feilong Li, Silvan Scheller, Michael Lienemann   +2 more
doaj   +1 more source

Selenocysteine in Trypanosoma evansi: Identification of the Genes selb, selc, seld, pstk, seltryp and the Selenophosphate Synthetase Protein

Acta Protozoologica, 2021
Selenoproteins have been described in all three domains of life and their function has been mainly associated with oxidative stress defense.
Kaio Cesar Simiano Tavares, Maria Gabriela Casagrande Dambrós, André Saraiva Leão Antunes, Pietro Martin Danziato, Patricia Hermes Stoco, Aline Daiane Schlindwein, Renato Simões Moreira, Luiz Claudio Miletti   +7 more
doaj   +1 more source

Assessment of reagents for selenocysteine conjugation and the stability of selenocysteine adducts [PDF]

Organic & Biomolecular Chemistry, 2016
The allenamide functional group reacts with selenocysteine with notably high efficiency, leads to antibody conjugates with remarkable stability, and shows exquisite selectivity for selenocysteine conjugation.
Lee, Pedzisa, Xiuling, Li, Christoph, Rader, William R, Roush   +3 more
openaire   +2 more sources

Delivery of the selenoprotein thioredoxin reductase 1 to mammalian cells

Frontiers in Molecular Biosciences, 2022
Over-expression of genetically encoded thioredoxin reductase 1 (TrxR1) TrxR1 can be toxic to cells due to the formation of a truncated version of the enzyme. We developed a new mammalian cell-based model to investigate TrxR1 activity.
David E. Wright, Tarana Siddika, Ilka U. Heinemann, Patrick O’Donoghue   +3 more
doaj   +1 more source

Human selenoprotein P and S variant mRNAs with different numbers of SECIS elements and inferences from mutant mice of the roles of multiple SECIS elements [PDF]

Open Biology, 2016
Dynamic redefinition of the 10 UGAs in human and mouse selenoprotein P (Sepp1) mRNAs to specify selenocysteine instead of termination involves two 3′ UTR structural elements (SECIS) and is regulated by selenium availability. In addition to the previously
Sen Wu, Marco Mariotti, Didac Santesmasses, Kristina E. Hill, Janinah Baclaocos, Estel Aparicio-Prat, Shuping Li, John Mackrill, Yuanyuan Wu, Michael T. Howard, Mario Capecchi, Roderic Guigó, Raymond F. Burk, John F. Atkins   +13 more
doaj   +1 more source

On the road to selenocysteine [PDF]

Proceedings of the National Academy of Sciences, 2004
Decades ago, a mammalian tRNA that exclusively bound the UGA stop codon was identified by using the triplet binding assay that was instrumental in deciphering the genetic code (1). The possibility of a naturally occurring suppressor tRNA presented the dilemma of explaining the biological function of such a molecule.
openaire   +2 more sources

Harnessing the Reactivity of Sulfinate Salts With Cystine: An Umpolung Approach to Residue‐Specific Peptide Modification

Angewandte Chemie, EarlyView.
While cysteine is widely employed as a nucleophilic handle for peptide modification, the electrophilicity of the oxidized form, cystine, is rarely exploited. This study describes a mild, photochemical coupling of sulfinate salts with peptide disulfides.
Joshua M. Hammond, Esteban Suárez‐Picado, Lena von Sydow, Hanna Bruss, Laurent Knerr, Magnus Johansson, Anaïs Noisier, Lara R. Malins   +7 more
wiley   +2 more sources

Crystal structures of the human elongation factor eEFSec suggest a non-canonical mechanism for selenocysteine incorporation

Nature Communications, 2016
Specialized translation elongation factors (eEFSec and SelB) promote selenocysteine incorporation into proteins. Here, the authors report the structure of human eEFSec, examine its interactions with guanine nucleotides, and propose a non-canonical ...
Malgorzata Dobosz-Bartoszek, Mark H. Pinkerton, Zbyszek Otwinowski, Srinivas Chakravarthy, Dieter Söll, Paul R. Copeland, Miljan Simonović   +6 more
doaj   +1 more source

Piperlongumine Inhibits Thioredoxin Reductase 1 by Targeting Selenocysteine Residues and Sensitizes Cancer Cells to Erastin

Antioxidants, 2022
Piperlongumine, a natural alkaloid substance extracted from the fruit of the long pepper (Piper longum Linn.), is known to inhibit the cytosolic thioredoxin reductase (TXNRD1 or TrxR1) and selectively kill cancer cells. However, the details and mechanism
Yijia Yang, Shibo Sun, Weiping Xu, Yue Zhang, Rui Yang, Kun Ma, Jie Zhang, Jianqiang Xu   +7 more
doaj   +1 more source

Evaluation of Chemical Strategies for Improving the Stability and Oral Toxicity of Insecticidal Peptides

Biomedicines, 2018
Spider venoms are a rich source of insecticidal peptide toxins. Their development as bioinsecticides has, however, been hampered due to concerns about potential lack of stability and oral bioactivity.
Volker Herzig, Aline Dantas de Araujo, Kathryn P. Greenwood, Yanni K.-Y. Chin, Monique J. Windley, Youmie Chong, Markus Muttenthaler, Mehdi Mobli, Neil Audsley, Graham M. Nicholson, Paul F. Alewood, Glenn F. King   +11 more
doaj   +1 more source