Results 41 to 50 of about 11,591 (176)

Deep Contrastive Learning for High‐Throughput Prediction of Drug Resistance Mutations from Sequences

Advanced Science, EarlyView.
This study presents DeepMutDTA, a deep learning framework aimed at predicting mutation‐induced changes in protein‐drug interactions and prioritizing variants potentially linked to drug resistance. Trained on large‐scale data, it incorporates SimSiam‐MuTF, a label‐aware contrastive fine‐tuning strategy that encourages separation between WT and MT ...
Xiaowen Hu, Pan Zhang, Shangqian Wu, Hao Sun, Minwei Li, Sophia Tsoka, Zizhang Sheng, Lei Deng   +7 more
wiley   +1 more source

Selenocysteine inserting tRNAs: an overview [PDF]

FEMS Microbiology Reviews, 1999
One of the recent discoveries in protein biosynthesis was the finding that selenocysteine, the 21st amino acid, is cotranslationally inserted into polypeptides under the direction of a UGA codon assisted by a specific structural signal in the mRNA. The key to selenocysteine biosynthesis and insertion is a special tRNA species, tRNA(Sec).
S, Commans, A, Böck
openaire   +2 more sources

Electrochemical Modification of Polypeptides at Selenocysteine

Angewandte Chemie, 2023
AbstractMild strategies for the selective modification of peptides and proteins are in demand for applications in therapeutic peptide and protein discovery, and in the study of fundamental biomolecular processes. Herein, we describe the development of an electrochemical selenoetherification (e‐SE) platform for the efficient site‐selective ...
Angus S. Mackay, Joshua W. C. Maxwell, Max J. Bedding, Sameer S. Kulkarni, Stephen A. Byrne, Lucas Kambanis, Mihai V. Popescu, Robert S. Paton, Lara R. Malins, Anneliese S. Ashhurst, Leo Corcilius, Richard J. Payne   +11 more
openaire   +2 more sources

Human selenoprotein P and S variant mRNAs with different numbers of SECIS elements and inferences from mutant mice of the roles of multiple SECIS elements [PDF]

Open Biology, 2016
Dynamic redefinition of the 10 UGAs in human and mouse selenoprotein P (Sepp1) mRNAs to specify selenocysteine instead of termination involves two 3′ UTR structural elements (SECIS) and is regulated by selenium availability. In addition to the previously
Sen Wu, Marco Mariotti, Didac Santesmasses, Kristina E. Hill, Janinah Baclaocos, Estel Aparicio-Prat, Shuping Li, John Mackrill, Yuanyuan Wu, Michael T. Howard, Mario Capecchi, Roderic Guigó, Raymond F. Burk, John F. Atkins   +13 more
doaj   +1 more source

Selenium Nanoparticles Selectively Target KRAS G13D to Inhibit Colorectal Cancer

Advanced Science, EarlyView.
The mechanisms of SeNPs therapy in cancer treatment, encompass three parallel actions: (1) seleno‐amino acids, key metabolites, upregulate GPX2 expression, thereby inhibiting tumor metastasis via the GPX2‐HIF1α‐VEGF signaling pathway; (2) selenite (SeO32−), an inorganic metabolite, forms hydrogen bonds with amino acid residues 13–17 of the KRAS G13D ...
Xiaoting Liu, Chong Wu, Shiyao Song, Li Ma, Shaoqing Huang, Yuting He, Qinghai Li, Yanzhou Chang, Dan Jiang, Yong Mei, Junchao Cai, Zhenshuang Du, Tianfeng Chen, Weiling He   +13 more
wiley   +1 more source

Microbial distribution of selenocysteine lyase [PDF]

Journal of Bacteriology, 1983
We studied the distribution of selenocysteine lyase, a novel enzyme catalyzing the conversion of selenocysteine into alanine and H2Se, which we first demonstrated in various mammalian tissues (Esaki et al., J. Biol. Chem. 257:4386-4391, 1982). Enzyme activity was found in various bacteria such as Alcaligenes viscolactis and Pseudomonas alkanolytica. No
P, Chocat, N, Esaki, T, Nakamura, H, Tanaka, K, Soda   +4 more
openaire   +2 more sources

Identification of the Selenoprotein S Positive UGA Recoding (SPUR) element and its position-dependent activity

RNA Biology
Selenoproteins are a unique class of proteins that contain the 21st amino acid, selenocysteine (Sec). Addition of Sec into a protein is achieved by recoding of the UGA stop codon. All 25 mammalian selenoprotein mRNAs possess a 3′ UTR stem-loop structure,
Eric M. Cockman, Vivek Narayan, Belinda Willard, Sumangala P. Shetty, Paul R. Copeland, Donna M. Driscoll   +5 more
doaj   +1 more source

A New Recruitable E3 Ligase UHRF1 Supporting Targeted Protein Degradation: A Minimal Azide as a Recruitment Ligand

Advanced Science, EarlyView.
Targeted protein degradation is often limited by the scarcity of usable E3 ligases. Herein, we report the first small‐molecule GPX4 degraders that incorporate either electrophilic warheads or a minimal azide group as an E3 recruitment ligand. The azide‐based degrader DK‐5070 effectively drives potent GPX4 degradation; mechanistic studies reveal that ...
Zehong Lin, Kang Duan, Rui Wan, Tao Jiang, Jinzhe Wei, Miao Sun, Mariia A. Skryl'nikova, Maxim A. Gureev, Zhi‐Min Zhang, Zhang Zhang, Ke Ding, Nan Ma, Tongzheng Liu, Yi Tan, Zhengqiu Li   +14 more
wiley   +1 more source

Biotransformation of Selenium by Lactic Acid Bacteria: Formation of Seleno-Nanoparticles and Seleno-Amino Acids

Frontiers in Bioengineering and Biotechnology, 2020
Selenium (Se) is an essential micronutrient for the majority of living organisms, and it has been identified as selenocysteine in the active site of several selenoproteins such as glutathione peroxidase, thioredoxin reductase, and deiodinases.
Fernando Gabriel Martínez, Fernando Gabriel Martínez, Gustavo Moreno-Martin, Micaela Pescuma, Yolanda Madrid-Albarrán, Fernanda Mozzi   +5 more
doaj   +1 more source

Piperlongumine Inhibits Thioredoxin Reductase 1 by Targeting Selenocysteine Residues and Sensitizes Cancer Cells to Erastin

Antioxidants, 2022
Piperlongumine, a natural alkaloid substance extracted from the fruit of the long pepper (Piper longum Linn.), is known to inhibit the cytosolic thioredoxin reductase (TXNRD1 or TrxR1) and selectively kill cancer cells. However, the details and mechanism
Yijia Yang, Shibo Sun, Weiping Xu, Yue Zhang, Rui Yang, Kun Ma, Jie Zhang, Jianqiang Xu   +7 more
doaj   +1 more source