Results 1 to 10 of about 53,984 (298)

Synaptic proteins promote calcium-triggered fast transition from point contact to full fusion [PDF]

eLife, 2012
The molecular underpinnings of synaptic vesicle fusion for fast neurotransmitter release are still unclear. Here, we used a single vesicle–vesicle system with reconstituted SNARE and synaptotagmin-1 proteoliposomes to decipher the temporal sequence of ...
Jiajie Diao, Patricia Grob, Daniel J Cipriano, Minjoung Kyoung, Yunxiang Zhang, Sachi Shah, Amie Nguyen, Mark Padolina, Ankita Srivastava, Marija Vrljic, Ankita Shah, Eva Nogales, Steven Chu, Axel T Brunger   +13 more
doaj   +3 more sources

Alpha-Synuclein and Calpains Disrupt SNARE-Mediated Synaptic Vesicle Fusion During Manganese Exposure in SH-SY5Y Cells [PDF]

Cells, 2018
Synaptic vesicle fusion is mediated by an assembly of soluble N-ethylmaleimide-sensitive fusion protein attachment protein receptors (SNAREs), composed of syntaxin 1, soluble NSF-attachment protein (SNAP)-25, and synaptobrevin-2/VAMP-2.
Can Wang, Zhuo Ma, Dong-Ying Yan, Chang Liu, Yu Deng, Wei Liu, Zhao-Fa Xu, Bin Xu   +7 more
doaj   +2 more sources

A novel synaptic vesicle fusion path in the rat cerebral cortex: the "saddle" point hypothesis. [PDF]

PLoS ONE, 2014
We improved freeze-fracture electron microscopy to study synapses in the neuropil of the rat cerebral cortex at ∼2 nm resolution and in three-dimensions.
Guido A Zampighi, Raul Serrano, Julio L Vergara   +2 more
doaj   +2 more sources

Impaired docking and recycling of synaptic vesicles in inherited lysosomal sphingolipidoses [PDF]

Cell Communication and Signaling
Cognitive, memory, and learning impairments are common features of many lysosomal sphingolipidoses, yet the underlying synaptic mechanisms remain poorly defined. Here, we examined the impact of galactosylceramidase (GALC) deficiency on synaptic structure
Diego Zelada, Natalia Saldivia, Ashna Konjeti, Shayla Samano, Michael S. Marshall, Rima Rebiai, Pearl Sutter, Zack Zurawski, Daniel Simchuk, Ruth Muchiri, Richard van Breemen, Daesung Shin, Maria I. Givogri, Stephen J. Crocker, Simon Alford, Ernesto R. Bongarzone   +15 more
doaj   +2 more sources

Post-tetanic potentiation lowers the energy barrier for synaptic vesicle fusion independently of Synaptotagmin-1 [PDF]

eLife, 2020
Previously, we showed that modulation of the energy barrier for synaptic vesicle fusion boosts release rates supralinearly (Schotten, 2015). Here we show that mouse hippocampal synapses employ this principle to trigger Ca2+-dependent vesicle release and ...
Vincent Huson, Marieke Meijer, Rien Dekker, Mirelle ter Veer, Marvin Ruiter, Jan RT van Weering, Matthijs Verhage, Lennart Niels Cornelisse   +7 more
doaj   +2 more sources

Otoferlin acts as a Ca2+ sensor for vesicle fusion and vesicle pool replenishment at auditory hair cell ribbon synapses [PDF]

eLife, 2017
Hearing relies on rapid, temporally precise, and sustained neurotransmitter release at the ribbon synapses of sensory cells, the inner hair cells (IHCs).
Nicolas Michalski, Juan D Goutman, Sarah Marie Auclair, Jacques Boutet de Monvel, Margot Tertrais, Alice Emptoz, Alexandre Parrin, Sylvie Nouaille, Marc Guillon, Martin Sachse, Danica Ciric, Amel Bahloul, Jean-Pierre Hardelin, Roger Bryan Sutton, Paul Avan, Shyam S Krishnakumar, James E Rothman, Didier Dulon, Saaid Safieddine, Christine Petit   +19 more
doaj   +3 more sources

Two successive oligomeric Munc13 assemblies scaffold vesicle docking and SNARE assembly to support neurotransmitter release [PDF]

Nature Communications
The critical protein Munc13 serves numerous roles in the docking and priming of synaptic vesicles. On the presynaptic plasma membrane, Munc13 is organized into nanoclusters corresponding to release sites where synaptic vesicles dock and fuse. However, it
Manindra Bera, Kirill Grushin, R. Venkat Kalyana Sundaram, Jasmine S. Hinzen, Joyce Chen, Atrouli Chatterjee, Abhijith Radhakrishnan, Seong Lee, Murugesh Padmanarayana, Jeff Coleman, Frédéric Pincet, James E. Rothman, Jeremy S. Dittman   +12 more
doaj   +2 more sources

Amyloid-β acts as a regulator of neurotransmitter release disrupting the interaction between synaptophysin and VAMP2. [PDF]

, 2012
BACKGROUND: It is becoming increasingly evident that deficits in the cortex and hippocampus at early stages of dementia in Alzheimer's disease (AD) are associated with synaptic damage caused by oligomers of the toxic amyloid-β peptide (Aβ42).
Alifragis, P, Austen, BM, Beesley, PW, Empson, RM, Russell, CL, Semerdjieva, S   +5 more
core   +16 more sources

Calcium dependence of neurotransmitter release at a high fidelity synapse

eLife, 2021
The Ca2+-dependence of the priming, fusion, and replenishment of synaptic vesicles are fundamental parameters controlling neurotransmitter release and synaptic plasticity.
Abdelmoneim Eshra, Hartmut Schmidt, Jens Eilers, Stefan Hallermann   +3 more
doaj   +1 more source

Room for Two: The Synaptophysin/Synaptobrevin Complex

Frontiers in Synaptic Neuroscience, 2021
Synaptic vesicle release is regulated by upwards of 30 proteins at the fusion complex alone, but disruptions in any one of these components can have devastating consequences for neuronal communication. Aberrant molecular responses to calcium signaling at
Dustin N. White, Michael H. B. Stowell
doaj   +1 more source