Direct IgG epitope mapping on bacterial AB toxins by cryo-EM
STAR Protocols, 2021 This cryo-EM protocol was used to determine the B cell epitope map on the CdtB subunit of typhoid toxin, an A2B5 toxin secreted by Salmonella Typhi during infection. Immunoglobulin G (IgG) was directly mixed with typhoid toxin in this protocol, different from our previous cryo-EM protocol that uses the Fab fragments in place of IgG.
Tri Nguyen, Jeongmin Song
openaire +3 more sources
AIP56, an AB toxin secreted by <i>Photobacterium damselae</i> subsp. <i>piscicida</i>, has tropism for myeloid cells. [PDF]
Front ImmunolAbstract The AB-type toxin AIP56 is a key virulence factor of Photobacterium damselae subsp. piscicida ( Phdp ), inducing apoptosis in fish immune cells.
Freitas IL +5 more
europepmc +5 more sources
Structural insights into Clostridium perfringens delta toxin pore formation [PDF]
, 2013 Clostridium perfringens Delta toxin is one of the three hemolysin-like proteins produced by C. perfringens type C and possibly type B strains. One of the others, NetB, has been shown to be the major cause of Avian Nectrotic Enteritis, which following the
Claire E Naylor +37 more
core +1 more source
Sensitivity of Kupffer cells and liver sinusoidal endothelial cells to ricin toxin and ricin toxin–Ab complexes [PDF]
Journal of Leukocyte Biology, 2019 Abstract Ricin toxin is a plant-derived, ribosome-inactivating protein that is rapidly cleared from circulation by Kupffer cells (KCs) and liver sinusoidal endothelial cells (LSECs)—with fatal consequences. Rather than being inactivated, ricin evades normal degradative pathways and kills both KCs and LSECs with remarkable efficiency ...
Bridget Mooney +4 more
openaire +2 more sources
Sensors, 2010 This study describes the direct detection of the biological toxin (Ricin) in acidic environment without pH adjustment by hydrophobically modified electrochemical impedance immunosensor (EII). The nano-porous aluminum substrate for EII was hydrophobically
Changhoon Chai +2 more
doaj +1 more source
Gas phase characterization of the noncovalent quaternary structure of Cholera toxin and the Cholera toxin B subunit pentamer [PDF]
, 2006 Cholera toxin (CTx) is an AB5 cytotonic protein that has medical relevance in cholera and as a novel mucosal adjuvant. Here, we report an analysis of the noncovalent homopentameric complex of CTx B chain (CTx B5) using electrospray ionization triple ...
Jennings, Keith R. +15 more
core +1 more source
Inhibition of development of Shiga toxin-converting bacteriophages by either treatment with citrate or amino acid starvation [PDF]
, 2012 Objectives: Shiga toxin–producing Escherichia coli (STEC) are pathogenic strains, whose virulence depends on induction of Shiga toxin–converting prophages and their subsequent lytic development.
Bożena Nejman-Faleńczyk +9 more
core +2 more sources
PLoS ONE, 2012 Antitoxins are needed that can be produced economically with improved safety and shelf life compared to conventional antisera-based therapeutics. Here we report a practical strategy for development of simple antitoxin therapeutics with substantial ...
Jean Mukherjee +11 more
doaj +1 more source
Designed Azolopyridinium Salts Block Protective Antigen Pores In Vitro and Protect Cells from Anthrax Toxin [PDF]
, 2013 Background:Several intracellular acting bacterial protein toxins of the AB-type, which are known to enter cells by endocytosis, are shown to produce channels.
Barth, Holger +24 more
core +2 more sources
Cholera toxin B subunits assemble into pentamers--proposition of a fly-casting mechanism. [PDF]
PLoS ONE, 2010 The cholera toxin B pentamer (CtxB(5)), which belongs to the AB(5) toxin family, is used as a model study for protein assembly. The effect of the pH on the reassembly of the toxin was investigated using immunochemical, electrophoretic and spectroscopic ...
Jihad Zrimi +4 more
doaj +1 more source