Results 21 to 30 of about 66,837 (297)

AB Toxins as High-Affinity Ligands for Cell Targeting in Cancer Therapy

International Journal of Molecular Sciences, 2023
Conventional targeted therapies for the treatment of cancer have limitations, including the development of acquired resistance. However, novel alternatives have emerged in the form of targeted therapies based on AB toxins. These biotoxins are a diverse group of highly poisonous molecules that show a nanomolar affinity for their target cell receptors ...
Márquez López, Ana, López Fanarraga, Mónica   +1 more
openaire   +3 more sources

AB₅-Type Toxin as a Pentameric Scaffold in Recombinant Vaccines against the Japanese Encephalitis Virus

Toxins, 2023
Japanese encephalitis virus (JEV) is an enveloped icosahedral capsid virus with a prime neutralizing epitope present in E protein domain III (EDIII). E dimers are rearranged into a five-fold symmetry of icosahedrons. Cholera toxin B (CTB) and heat-labile
Jina Ahn, Ji Eun Yu, Hanna Kim, Jemin Sung, Gyoonhee Han, Myung Hyun Sohn, Baik-Lin Seong   +6 more
doaj   +1 more source

Screening and activity identification of an anti-idiotype nanobody for Bt Cry1F toxin from the camelid naive antibody phage display library

Food and Agricultural Immunology, 2020
Anti-idiotypic antibody technology has been widely developed. Here, we prepared the F(ab’)2 fragments of the Cry1F toxin polyclonal antibody as Ab1and screened an anti-idiotype nanobody (VHH) for Bt Cry1F toxin from the camelid naive antibody phage ...
Jia Hao, Yihang Li, Jingxuan Wang, Chongxin Xu, Meijing Gao, Wei Chen, Xiao Zhang, Xiaodan Hu, Yuan Liu, Xianjin Liu   +9 more
doaj   +1 more source

Direct IgG epitope mapping on bacterial AB toxins by cryo-EM

STAR Protocols, 2021
This cryo-EM protocol was used to determine the B cell epitope map on the CdtB subunit of typhoid toxin, an A2B5 toxin secreted by Salmonella Typhi during infection. Immunoglobulin G (IgG) was directly mixed with typhoid toxin in this protocol, different from our previous cryo-EM protocol that uses the Fab fragments in place of IgG.
Tri Nguyen, Jeongmin Song
openaire   +3 more sources

Designed Azolopyridinium Salts Block Protective Antigen Pores In Vitro and Protect Cells from Anthrax Toxin [PDF]

, 2013
Background:Several intracellular acting bacterial protein toxins of the AB-type, which are known to enter cells by endocytosis, are shown to produce channels.
Barth, Holger, Anika Bronnhuber, Benz, Roland, Hajós György, Markus Huber-Lang, Hajós, György, Kerstin Duscha, Riedl Zsuzsanna, Beitzinger, Christoph, Riedl, Zsuzsanna, Duscha Kerstin, György Hajós, Huber-Lang Markus, Beitzinger Christoph, Barth Holger, Duscha, Kerstin, Bronnhuber, Annika, Benz Roland, Christoph Beitzinger, Roland Benz, Holger Barth, Bronnhuber Anika, Bronnhuber, Anika, Huber-Lang, Markus, Zsuzsanna Riedl   +24 more
core   +1 more source

Sensitivity of Kupffer cells and liver sinusoidal endothelial cells to ricin toxin and ricin toxin–Ab complexes [PDF]

Journal of Leukocyte Biology, 2019
Abstract Ricin toxin is a plant-derived, ribosome-inactivating protein that is rapidly cleared from circulation by Kupffer cells (KCs) and liver sinusoidal endothelial cells (LSECs)—with fatal consequences. Rather than being inactivated, ricin evades normal degradative pathways and kills both KCs and LSECs with remarkable efficiency ...
Bridget Mooney, Fernando J Torres-Velez, Jennifer Doering, Dylan J Ehrbar, Nicholas J Mantis   +4 more
openaire   +2 more sources

The association of shiga-like toxin with detergent-resistant membranes is modulated by glucosylceramide and is an essential requirement in the endoplasmic reticulum for a cytotoxic effect [PDF]

, 2006
Receptor-mediated internalization to the endoplasmic reticulum (ER) and subsequent retro-translocation to the cytosol are essential sequential processes required for the productive intoxication of susceptible mammalian cells by Shiga-like toxin-1 (SLTx).
Falguières, T, Platt, Frances M., Platt, Frances, Roberts, LM, Sillence, DJ, Smith, Daniel C., Jarvis, Rosemary M., Lord, JM, Smith, DC, Lord, J. Michael, Roberts, Lynne M., Jarvis, R. M., Sillence, Daniel J., Johannes, Ludger, Falguires, T., Falguières, Thomas, Lord, Mike, Johannes, L, Roberts, L. M. (Lynne M.), Jarvis, RM, Smith, D. C.   +20 more
core   +1 more source

A novel strategy for development of recombinant antitoxin therapeutics tested in a mouse botulism model.

PLoS ONE, 2012
Antitoxins are needed that can be produced economically with improved safety and shelf life compared to conventional antisera-based therapeutics. Here we report a practical strategy for development of simple antitoxin therapeutics with substantial ...
Jean Mukherjee, Jacqueline M Tremblay, Clinton E Leysath, Kwasi Ofori, Karen Baldwin, Xiaochuan Feng, Daniela Bedenice, Robert P Webb, Patrick M Wright, Leonard A Smith, Saul Tzipori, Charles B Shoemaker   +11 more
doaj   +1 more source

Cholera toxin B subunits assemble into pentamers--proposition of a fly-casting mechanism. [PDF]

PLoS ONE, 2010
The cholera toxin B pentamer (CtxB(5)), which belongs to the AB(5) toxin family, is used as a model study for protein assembly. The effect of the pH on the reassembly of the toxin was investigated using immunochemical, electrophoretic and spectroscopic ...
Jihad Zrimi, Alicia Ng Ling, Ernawati Giri-Rachman Arifin, Giovanni Feverati, Claire Lesieur   +4 more
doaj   +1 more source

Cytolethal Distending Toxin: A Unique Variation on the AB Toxin Paradigm [PDF]

New Journal of Science, 2014
Some of the most potent toxins produced by plants and bacteria are members of a large family known as the AB toxins. AB toxins are generally characterized by a heterogenous complex consisting of two protein chains arranged in various monomeric or polymeric configurations. The newest class within this superfamily is the cytolethal distending toxin (Cdt).
openaire   +1 more source