Results 51 to 60 of about 232 (115)
Cell-Free Systems Enable the Production of AB5 Toxins for Diagnostic Applications
Toxins, 2022 Cell-free protein synthesis (CFPS) represents a versatile key technology for the production of toxic proteins. As a cell lysate, rather than viable cells, is used, the toxic effects on the host organism can be circumvented. The open nature of cell-free systems allows for the addition of supplements affecting protein concentration and folding.
Franziska Ramm +5 more
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Laboratory diagnostic methods for clostridioides difficile infection
One Health & Risk Management, 2023 Introduction. Clostridioides difficile is an anaerobic gram-positive, sporeforming, toxin-producing bacillus that is transmitted among humans through the fecal-oral route, as a result of ingestion of spores. Colonization of C.
Anastasia ALEXANDROV
doaj
Modulation of toxin stability by 4-phenylbutyric acid and negatively charged phospholipids. [PDF]
PLoS ONE, 2011 AB toxins such as ricin and cholera toxin (CT) consist of an enzymatic A domain and a receptor-binding B domain. After endocytosis of the surface-bound toxin, both ricin and CT are transported by vesicle carriers to the endoplasmic reticulum (ER).
Supriyo Ray +4 more
doaj +1 more source
EFFECT OF AFLATOXINS ON GOATS MILK PRODUCTION AND ITS CHEMICAL AND PHYSICAL COMPOSITION [PDF]
Mesopotamia Journal of Agriculture, 2008 This study was conducted to show the effects of each aflatoxins (AF) or activated sodum bentonite(AB) .Singly or combination in the following groups:1- animals fed Diet (AF) alone at 2.5mg/kg.2-animal fed died (AF)at 2.5mg/kgwith AB(5g/kg).
doaj +1 more source
Biochimica et Biophysica Acta (BBA) - Biomembranes, 2011 Shiga toxin (STx) belongs to the AB(5) toxin family and is transiently localized in the periplasm before secretion into the extracellular milieu. While producing outer membrane vesicles (OMVs) containing only A subunit of the toxin (STxA), we created specific STx1B- and STx2B-deficient mutants of E. coli O157:H7.
Kim, SH Kim, Sang-Hyun +11 more
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Structural investigation of the Yersinia pseudotuberculosis AB-toxin CNFy
, 2015 Yersinia cytotoxic necrotizing factor (CNFy) is a recently discovered AB-toxin essential for the invasion process of Yersinia. Due to its ability to deamidate and thereby constitutively activate small Rho-GTPases CNFy was combined with CNF1 to CNF3 of E.
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BiP Negatively Affects Ricin Transport
Toxins, 2013 The AB plant toxin ricin binds both glycoproteins and glycolipids at the cell surface via its B subunit. After binding, ricin is endocytosed and then transported retrogradely through the Golgi to the endoplasmic reticulum (ER).
Kirsten Sandvig +4 more
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Cell Reports, 2013 Anthrax lethal toxin is a classical AB toxin comprised of two components: protective antigen (PA) and lethal factor (LF). Here, we show that following assembly and endocytosis, PA forms a channel that translocates LF, not only into the cytosol, but also ...
Laurence Abrami +6 more
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PLoS ONE, 2011 Cholera toxin (CT) travels as an intact AB(5) protein toxin from the cell surface to the endoplasmic reticulum (ER) of an intoxicated cell. In the ER, the catalytic A1 subunit dissociates from the rest of the toxin.
Michael Taylor +8 more
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Toxins, 2015 Protective immunity against anthrax is inferred from measurement of vaccine antigen-specific neutralizing antibody titers in serum samples. In animal models, in vivo challenges with toxin and/or spores can also be performed.
T. Scott Devera +4 more
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