Results 81 to 90 of about 232 (115)

AB5 toxins: structures and inhibitor design

Current Opinion in Structural Biology, 2000
High-resolution crystal structures of AB(5) toxins in their native form or in complex with a variety of ligands have led to the structure-based design and discovery of inhibitors targeting different areas of the toxins. The most significant progress is the development of highly potent multivalent ligands that block binding of the toxins to their ...
E, Fan, E A, Merritt, C L, Verlinde, W G, Hol   +3 more
openaire   +2 more sources

Ab InitioStructure Determination and Refinement of a Scorpion Protein Toxin

Acta Crystallographica Section D Biological Crystallography, 1997
The structure of toxin II from the scorpion Androctonus australis Hector has been determined ab initio by direct methods using SnB at 0.96 A resolution. For the purpose of this structure redetermination, undertaken as a test of the minimal function and the SnB program, the identity and sequence of the protein was withheld from part of the research team.
G D, Smith, R H, Blessing, S E, Ealick, J C, Fontecilla-Camps, H A, Hauptman, D, Housset, D A, Langs, R, Miller   +7 more
openaire   +2 more sources

15 Membrane translocation by bacterial AB toxins

2002
Publisher Summary This chapter presents some of the basic experimental assays that have been developed or used in laboratories for the study of cholera and related E. coli heat labile enterotoxins, diphtheria toxin, and anthrax toxins. The chapter emphasizes on the importance of experimental systems that provide a high degree of temporal resolution ...
Kamran Badizadegan, R.John Collier, Wayne I. Lencer   +2 more
openaire   +1 more source

Purification and some properties ofClostridium botulinumtype AB toxin

FEMS Microbiology Letters, 1986
The progenitor toxin of Clostridium botulinum type AB was purified; both large-sized (L) and medium-sized (M) toxins were found. The toxicity of M toxin increased by about 10-fold upon trypsinization; the increase was due mostly to type B toxin and a little to type A toxin.
Genji Sakaguchi, Sumiko Sakaguchi, Shunji Kozaki, Motohide Takahashi   +3 more
openaire   +1 more source

A Cell-Based Fluorescent Assay to Detect the Activity of AB Toxins that Inhibit Protein Synthesis

2017
Many AB toxins elicit a cytotoxic effect involving the inhibition of protein synthesis. In this chapter, we describe a simple cell-based fluorescent assay to detect and quantify the inhibition of protein synthesis. The assay can also identify and characterize toxin inhibitors.
Cherubin, Patrick, Quiñones, Beatriz, Elkahoui, Salem, Yokoyama, Wallace, Teter, Ken   +4 more
openaire   +3 more sources

Multivalent Inhibition of AB ₅ Toxins

Journal of the American Chemical Society, 2001
J M, Gargano, T, Ngo, J Y, Kim, D W, Acheson, W J, Lees   +4 more
openaire   +2 more sources

Calibration of Colorimetric Protein Assays for Quantitation of Plant AB Toxins

Analytical Biochemistry, 2000
M, Jiménez, S, André, H J, Gabius, D, Solís   +3 more
openaire   +2 more sources

Biology and evolution of bacterial toxin–antitoxin systems

Nature Reviews Microbiology, 2022
Nathan Fraikin, Laurence Van Melderen, Frédéric Goormaghtigh   +2 more
exaly  

Cloning, expression, purification and preliminary X-ray diffraction studies of a novel AB₅ toxin.

Acta crystallographica. Section F, Structural biology and crystallization communications, 2013
AB₅ toxins are key virulence factors found in a range of pathogenic bacteria. AB₅ toxins consist of two components: a pentameric B subunit that targets eukaryotic cells by binding to glycans located on the cell surface and a catalytic A subunit that disrupts host cellular function following internalization.
Natasha, Ng, Dene, Littler, Jérôme, Le Nours, Adrienne W, Paton, James C, Paton, Jamie, Rossjohn, Travis, Beddoe   +6 more
openaire   +1 more source

Toxin-Antitoxin Systems as Phage Defense Elements

Annual Review of Microbiology, 2022
Michael T Laub, Michele Leroux
exaly