Results 11 to 20 of about 5,817 (208)

Rare antibody phage isolation and discrimination (RAPID) biopanning enables identification of high-affinity antibodies against challenging targets

open access: yesCommunications Biology, 2023
In vitro biopanning platforms using synthetic phage display antibody libraries have enabled the identification of antibodies against antigens that were once thought to be beyond the scope of immunization.
Dong hee Chung   +11 more
doaj   +1 more source

Development of an Immunoassay Detection System for Koi Herpesvirus Using Recombinant Single-Chain Variable Fragments

open access: yesFishes, 2022
Koi herpesvirus (KHV) is a highly contagious virus that causes high mortality in koi and common carp, leading to a reduction in production worldwide.
Haneul Seo   +5 more
doaj   +1 more source

BDB: biopanning data bank [PDF]

open access: yesNucleic Acids Research, 2015
The BDB database (http://immunet.cn/bdb) is an update of the MimoDB database, which was previously described in the 2012 Nucleic Acids Research Database issue. The rebranded name BDB is short for Biopanning Data Bank, which aims to be a portal for biopanning results of the combinatorial peptide library. Last updated in July 2015, BDB contains 2904 sets
He, Bifang   +16 more
openaire   +2 more sources

Analysis of Compositional Bias in a Commercial Phage Display Peptide Library by Next-Generation Sequencing

open access: yesViruses, 2022
The principal presumption of phage display biopanning is that the naïve library contains an unbiased repertoire of peptides, and thus, the enriched variants derive from the affinity selection of an entirely random peptide pool.
Ane Beth Sloth   +6 more
doaj   +1 more source

A Competitive Panning Method Reveals an Anti-SARS-CoV-2 Nanobody Specific for an RBD-ACE2 Binding Site

open access: yesVaccines, 2023
Most neutralizing antibodies neutralize the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) by directly blocking the interactions between the spike glycoprotein receptor-binding domain (RBD) and its receptor, human angiotensin-converting ...
Siqi He   +6 more
doaj   +1 more source

Quantitative PCR of T7 Bacteriophage from Biopanning [PDF]

open access: yesJournal of Visualized Experiments, 2018
This protocol describes the use of quantitative PCR (qPCR) to enumerate T7 phages from phage selection experiments (i.e., "biopanning"). qPCR is a fluorescence-based approach to quantify DNA, and here, it is adapted to quantify phage genomes as a proxy for phage particles.
Xiujuan, Peng   +4 more
openaire   +2 more sources

Native llama Nanobody Library Panning Performed by Phage and Yeast Display Provides Binders Suitable for C-Reactive Protein Detection

open access: yesBiosensors, 2021
C-reactive protein (CRP) is an inflammation biomarker that should be quantified accurately during infections and healing processes. Nanobodies are good candidates to replace conventional antibodies in immunodiagnostics due to their inexpensive production,
Sandra Oloketuyi   +9 more
doaj   +1 more source

Antibodies to Variable Domain 4 Linear Epitopes of the Chlamydia trachomatis Major Outer Membrane Protein Are Not Associated with Chlamydia Resolution or Reinfection in Women

open access: yesmSphere, 2020
Chlamydia trachomatis is an obligate intracellular bacterium. C. trachomatis infection is the most prevalent bacterial sexually transmitted infection and can lead to pelvic inflammatory disease and infertility in women. There is no licensed vaccine for C.
Amanda L. Collar   +7 more
doaj   +1 more source

VNAR development through antigen immunization of Japanese topeshark (Hemitriakis japanica)

open access: yesFrontiers in Bioengineering and Biotechnology, 2023
The VNAR (Variable New Antigen Receptor) is the smallest single-domain antibody derived from the variable domain of IgNAR of cartilaginous fishes. Despite its biomedical and diagnostic potential, research on VNAR has been limited due to the difficulties ...
Hiroyuki Takeda   +13 more
doaj   +1 more source

An overview on application of phage display technique in immunological studies

open access: yesAsian Pacific Journal of Tropical Biomedicine, 2017
Phage display is very strong technique in drug discovery and development. Phage display has many applications in improving the immunological studies. Development of monoclonal antibody, peptides, peptidomimetics and epitope mapping are main application ...
Abbas Rami   +4 more
doaj   +1 more source

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