Biomedicines, 2022 The complement system plays a key role in myasthenia gravis (MG). Anti-complement drugs are emerging as effective therapies to treat anti-acetylcholine receptor (AChR) antibody-positive MG patients, though their usage is still limited by the high costs ...
Nicola Iacomino +11 more
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Quantitative detection of red blood cell antibody-mediated complement activation
Zhongguo shuxue zazhi, 2022 Objective To construct an in-vitro model of erythrocyte antibody-mediated complement activation, and establish quantitative detection methods based on flow cytometry and spectrophotometry, so as to explore the correlation of anti-body titers and ...
Zhongying WANG +9 more
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Purification of the human complement control protein C3b inactivator [PDF]
Biochemical Journal, 1980 An alternative method of isolation from human plasma is described for C3b inactivator, C3bINA, the proteinase that in conjunction with either beta 1H or C4b-binding protein will hydrolyse respectively C3b or C4b, the activation products of the third, C3 and fourth, C4, components of complement. The purification is by chromatography of plasma on columns
L G, Crossley, R R, Porter
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Human Tamm-Horsfall protein, a renal specific protein, serves as a cofactor in complement 3b degradation. [PDF]
PLoS ONE, 2017 Tamm-Horsfall protein (THP) is an abundant urinary protein of renal origin. We hypothesize that THP can act as an inhibitor of complement since THP binds complement 1q (C1q) of the classical complement pathway, inhibits activation of this pathway, and is
Diana C J Rhodes
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Insights Into the Structure-Function Relationships of Dimeric C3d Fragments
Frontiers in Immunology, 2021 Cleavage of C3 to C3a and C3b plays a central role in the generation of complement-mediated defences. Although the thioester-mediated surface deposition of C3b has been well-studied, fluid phase dimers of C3 fragments remain largely unexplored.
Ayla A. Wahid +21 more
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Inhibition of neutrophil function by fluid phase C3b of complement [PDF]
Infection and Immunity, 1983 A high-molecular-weight fragment of C3 was isolated from normal human serum by column chromatography, was generated by incubation of serum at 37 degrees C with inulin, and was produced from highly purified C3 by limited digestion with trypsin. This product was shown to inhibit the antibacterial function of neutrophils by using Escherichia coli O75 as ...
J D, Ogle, C K, Ogle, J W, Alexander
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Molecular Simulation Study on the Interaction between Porcine CR1-like and C3b
Molecules, 2023 The molecular basis of porcine red blood cell immune adhesion function stems from the complement receptor type 1-like (CR1-like) on its cell membrane.
Zhen Hou +6 more
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Thrombomodulin enhances complement regulation through strong affinity interactions with factor H and C3b-Factor H complex [PDF]
, 2016 Introduction Coagulation and complement systems are simultaneously activated at sites of tissue injury, leading to thrombin generation and opsonisation with C3b.
Baerga-Ortiz +58 more
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Factor H-related protein 4 activates complement by serving as a platform for the assembly of alternative pathway C3 convertase via its interaction with C3b protein [PDF]
, 2012 Human complement factor H-related protein (CFHR) 4 belongs to the factor H family of plasma glycoproteins that are composed of short consensus repeat (SCR) domains.
Hebecker, Mario, Józsi, Mihály
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Effect of complement-protein-C3b density on the binding of complement factor H to surface-bound C3b [PDF]
Biochemical Journal, 1991 Various amounts of the activation fragment C3b of the complement (C) protein C3 were coupled to Sepharose 4B by catalysis with the C3 convertase of the alternative pathway of C. The binding of radioactively labelled C proteins B and H (= factor H) to the C3b-carrying particles was assayed. It was found that the relative binding of H, but not of B, fell
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