Results 61 to 70 of about 8,203 (227)

Purification and characterization of the MspI DNA methyltransferase cloned and overexpressed in E. coli [PDF]

, 1992
The MspI restriction-modification system, which recognizes the sequence 5'-CCGG-3', has been previously cloned and sequenced (1). We subcloned the methyltransferase gene (M.MspI) downstream of the ptac promoter in the multicopy vector pUC119 and ...
Dubey, A. K., Mollet, B., Roberts, R. J.   +2 more
core   +1 more source

Molecular Characterization and Clinical Characteristics of m5C-Based RNA Methylation in Spinal Cord Injury: Validated by qPCR

International Journal of Genomics, 2022
Aberrant patterns of 5-methylcytosine (m5C)-based ribonucleic acid (RNA) methylation have critical roles in various human diseases, but their importance in spinal cord injury (SCI) is largely unknown.
Liang Cao, Wen Jun Pi, Qiang Zhang, Qing Li   +3 more
doaj   +1 more source

Comprehensive Analysis of 5-Methylcytosine (m5C) Regulators and the Immune Microenvironment in Pancreatic Adenocarcinoma to Aid Immunotherapy

Frontiers in Oncology, 2022
BackgroundPancreatic adenocarcinoma (PAAD) is one of the most malignant cancers and has a poor prognosis. As a critical RNA modification, 5-methylcytosine (m5C) has been reported to regulate tumor progression, including PAAD progression.
Ronglin Wang, Yongdong Guo, Peixiang Ma, Yang Song, Jie Min, Ting Zhao, Lei Hua, Chao Zhang, Cheng Yang, Jingjie Shi, Liaoliao Zhu, Dongxue Gan, Shanshan Li, Junqiang Li, Haichuan Su   +14 more
doaj   +1 more source

M.HhaI binds tightly to substrates containing mismatches at the target base [PDF]

, 1995
The (cytosine-5) DNA methyltransferase M.HhaI causes its target cytosine base to be flipped completely out of the DNA helix upon binding. We have investigated the effects of replacing the target cytosine by other, mismatched bases, including adenine ...
Klimasauskas, S., Roberts, R. J.
core   +1 more source

Transcriptome-Wide Annotation of m5C RNA Modifications Using Machine Learning [PDF]

Frontiers in Plant Science, 2018
The emergence of epitranscriptome opened a new chapter in gene regulation. 5-methylcytosine (m5C), as an important post-transcriptional modification, has been identified to be involved in a variety of biological processes such as subcellular localization and translational fidelity.
Jie Song, Jie Song, Jingjing Zhai, Enze Bian, Yujia Song, Jiantao Yu, Chuang Ma, Chuang Ma   +7 more
openaire   +3 more sources

Novel insights into the role of 5-Methylcytosine RNA methylation in human abdominal aortic aneurysm

Frontiers in Bioscience-Landmark, 2021
Background: It remains largely unclear about the function of 5-methylcytosine (m5C) RNA modification in the context of abdominal aortic aneurysm (AAA).
Yuchen He, Hao Zhang, Fanxing Yin, Panpan Guo, Shiyue Wang, Yihao Wu, Shijie Xin, Yanshuo Han, Jian Zhang   +8 more
doaj   +1 more source

Increased tRNA modification and gene-specific codon usage regulate cell cycle progression during the DNA damage response [PDF]

, 2012
S-phase and DNA damage promote increased ribonucleotide reductase (RNR) activity. Translation of RNR1 has been linked to the wobble uridine modifying enzyme tRNA methyltransferase 9 (Trm9).
Begley, Thomas J., Chan, Tsz Yan Clement, Dedon, Peter C., Dyavaiah, Madhu, Joseph, Fraulin, Patil, Ashish, Rooney, John P.   +6 more
core   +1 more source

m5C methylation modification guides the prognostic value and immune landscapes in acute myeloid leukemia

Aging, 2023
The development, incidence, and metastasis of tumors are all intimately correlated with 5-methylcytosine (m5C). However, uncertainty surrounds the function of m5C in acute myeloid leukemia (AML). In this study, multicenter AML data were collected and analyzed comprehensively to grasp the gene expression level, clinicopathological characteristics ...
Liu, Ya, Chen, Yiying, Cai, Maoping, Hong, Yunguang, Wu, Xiang, Li, Songyu   +5 more
openaire   +2 more sources

Recent insights into RNA m5C methylation modification in hepatocellular carcinoma

Biochimica et Biophysica Acta (BBA) - Reviews on Cancer
RNA 5-methylcytosine (m5C) methylation involves the addition of a methyl (-CH3) group to the cytosine (C) base within an RNA molecule, forming the m5C modification. m5C plays a role in numerous essential biological processes, including the regulation of RNA stability, nuclear export, and protein translation.
Danyang, Li, Yanyan, Liu, Guang, Yang, Mingyu, He, Ligong, Lu   +4 more
openaire   +2 more sources

Biological functions of 5-methylcytosine RNA-binding proteins and their potential mechanisms in human cancers

Frontiers in Oncology
The 5-methylcytosine (m5C) modification is a crucial epigenetic RNA modification, which is involved in the post-transcriptional regulation of genes. It plays an important role in various biological processes, including cell metabolism, growth, apoptosis,
Tingting Zhao, Zhe Zhang, Zhuo Chen, Guozheng Xu, Yongxi Wang, Fang Wang   +5 more
doaj   +1 more source