Results 11 to 20 of about 266,976 (317)

Microtubules and Microtubule-Associated Proteins [PDF]

Cold Spring Harbor Perspectives in Biology, 2018
Microtubules act as "railways" for motor-driven intracellular transport, interact with accessory proteins to assemble into larger structures such as the mitotic spindle, and provide an organizational framework to the rest of the cell. Key to these functions is the fact that microtubules are "dynamic." As with actin, the polymer dynamics are driven by ...
Holly V, Goodson, Erin M, Jonasson
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Nanomechanics of Microtubules [PDF]

Physical Review Letters, 2002
We have determined the mechanical anisotropy of a single microtubule by simultaneously measuring the Young's and the shear moduli in vitro. This was achieved by elastically deforming the microtubule deposited on a substrate tailored by electron-beam lithography with a tip of an atomic force microscope.
Kis, A, Kasas, S, Babic, B, Kulik, AJ, Benoit, W, Briggs, GAD, Schonenberger, C, Catsicas, S, Forro, L   +8 more
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Cortical anchoring of the microtubule cytoskeleton is essential for neuron polarity

eLife, 2020
The development of a polarized neuron relies on the selective transport of proteins to axons and dendrites. Although it is well known that the microtubule cytoskeleton has a central role in establishing neuronal polarity, how its specific organization is
Liu He, Robbelien Kooistra, Ravi Das, Ellen Oudejans, Eric van Leen, Johannes Ziegler, Sybren Portegies, Bart de Haan, Anna van Regteren Altena, Riccardo Stucchi, AF Maarten Altelaar, Stefan Wieser, Michael Krieg, Casper C Hoogenraad, Martin Harterink   +14 more
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Nde1 and Ndel1: Outstanding Mysteries in Dynein-Mediated Transport

Frontiers in Cell and Developmental Biology, 2022
Cytoplasmic dynein-1 (dynein) is the primary microtubule minus-end directed molecular motor in most eukaryotes. As such, dynein has a broad array of functions that range from driving retrograde-directed cargo trafficking to forming and focusing the ...
Sharon R. Garrott, Sharon R. Garrott, John P. Gillies, Morgan E. DeSantis, Morgan E. DeSantis   +4 more
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Self-assembly of pericentriolar material in interphase cells lacking centrioles

eLife, 2022
The major microtubule-organizing center (MTOC) in animal cells, the centrosome, comprises a pair of centrioles surrounded by pericentriolar material (PCM), which nucleates and anchors microtubules. Centrosome assembly depends on PCM binding to centrioles,
Fangrui Chen, Jingchao Wu, Malina K Iwanski, Daphne Jurriens, Arianna Sandron, Milena Pasolli, Gianmarco Puma, Jannes Z Kromhout, Chao Yang, Wilco Nijenhuis, Lukas C Kapitein, Florian Berger, Anna Akhmanova   +12 more
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Transforming growth factor-β2 induces morphological alteration of human corneal endothelial cells in vitro [PDF]

International Journal of Ophthalmology, 2014
AIM:To investigate the morphological altering effect of transforming growth factor-β2 (TGF-β2) on untransfected human corneal endothelial cells (HCECs) in vitro.METHODS: After untransfected HCECs were treated with TGF-β2 at different concentrations, the ...
Jing Wang, Ting-Jun Fan, Xiu-Xia Yang, Shi-Min Chang   +3 more
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Micron-scale geometrical features of microtubules as regulators of microtubule organization

eLife, 2021
The organization of micron-sized, multi-microtubule arrays from individual microtubules is essential for diverse cellular functions. The microtubule polymer is largely viewed as a passive building block during the organization process.
Nandini Mani, Sithara S Wijeratne, Radhika Subramanian   +2 more
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Isolation of Microtubules and Microtubule Proteins

Current Protocols in Cell Biology, 2008
AbstractThis unit describes various protocols for the isolation and purification of the main constituents of microtubules, chiefly α‐ and β‐tubulin, and the most significant microtubule associated proteins (MAPs), specifically MAP1A, MAP1B, MAP2, and tau.
Ávila, Jesús, Soares, Helena, Fanarraga, Mónica L., Zabala, Juan Carlos   +3 more
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Autoregulatory control of microtubule binding in doublecortin-like kinase 1

eLife, 2021
The microtubule-associated protein, doublecortin-like kinase 1 (DCLK1), is highly expressed in a range of cancers and is a prominent therapeutic target for kinase inhibitors. The physiological roles of DCLK1 kinase activity and how it is regulated remain
Regina L Agulto, Melissa M Rogers, Tracy C Tan, Amrita Ramkumar, Ashlyn M Downing, Hannah Bodin, Julia Castro, Dan W Nowakowski, Kassandra M Ori-McKenney   +8 more
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Actin–microtubule coordination at growing microtubule ends [PDF]

Nature Communications, 2014
To power dynamic processes in cells, the actin and microtubule cytoskeletons organize into complex structures. Although it is known that cytoskeletal coordination is vital for cell function, the mechanisms by which cross-linking proteins coordinate actin and microtubule activities remain poorly understood.
Preciado López, Magdalena, Huber, Florian, Grigoriev, Ilya, Steinmetz, Michel O., Akhmanova, Anna, Koenderink, Gijsje H., Dogterom, Marileen   +6 more
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