Analysis of CLIP and iCLIP methods for nucleotide-resolution studies of protein-RNA interactions. [PDF]
, 2012 UV cross-linking and immunoprecipitation (CLIP) and individual-nucleotide resolution CLIP (iCLIP) are methods to study protein-RNA interactions in untreated cells and tissues.
Curk, Tomaž +6 more
core +6 more sources
Emerging Microbes and Infections, 2023 5-Methylcytosine (m5C) is a widespread post-transcriptional RNA modification and is reported to be involved in manifold cellular responses and biological processes through regulating RNA metabolism.
Hongyun Wang +20 more
doaj +1 more source
Deciphering the epitranscriptome: a green perspective [PDF]
, 2016 The advent of high-throughput sequencing technologies coupled with new detection methods of RNA modifications has enabled investigation of a new layer of gene regulation - the epitranscriptome.
Burgess, A., David, R., Searle, I.
core +1 more source
NSUN2-mediated m5C modification of HBV RNA positively regulates HBV replication
PLOS Pathogens, 2023 Chronic hepatitis B virus (HBV) infection is a major cause of liver cirrhosis and liver cancer, despite strong prevention and treatment efforts. The study of the epigenetic modification of HBV has become a research hotspot, including the N6-methyladenosine (m6A) modification of HBV RNA, which plays complex roles in the HBV life cycle.
Jiangpeng Feng +16 more
openaire +3 more sources
Methylation by NSun2 Represses the Levels and Function of MicroRNA 125b [PDF]
Molecular and Cellular Biology, 2014 Methylation is a prevalent posttranscriptional modification of RNAs. However, whether mammalian microRNAs are methylated is unknown. Here, we show that the tRNA methyltransferase NSun2 methylates primary (pri-miR-125b), precursor (pre-miR-125b), and mature microRNA 125b (miR-125b) in vitro and in vivo.
Shuai, Yuan +12 more
openaire +2 more sources
Transcriptome-Wide Effects of Sphingosine Kinases Knockdown in Metastatic Prostate and Breast Cancer Cells: Implications for Therapeutic Targeting [PDF]
, 2019 Sphingosine kinases 1 and 2 (SK1 and SK2) are proto-oncogenic isozymes expressed in many human tumors and associated with chemoresistance and poor prognosis.
Alshaker +30 more
core +4 more sources
Blanks, a nuclear siRNA/dsRNA-binding complex component, is required for Drosophila spermiogenesis [PDF]
, 2011 Small RNAs and a diverse array of protein partners control gene expression in eukaryotes through a variety of mechanisms. By combining siRNA affinity chromatography and mass spectrometry, we have identified the double-stranded RNA-binding domain protein ...
Gerbasi, V. R. +5 more
core +1 more source
Unanchored tri-NEDD8 inhibits PARP-1 to protect from oxidative stress-induced cell death [PDF]
, 2019 NEDD8 is a ubiquitin‐like protein that activates cullin‐RING E3 ubiquitin ligases (CRLs). Here, we identify a novel role for NEDD8 in regulating the activity of poly(ADP‐ribose) polymerase 1 (PARP‐1) in response to oxidative stress.
Burchmore, Richard +6 more
core +4 more sources
Sex hormone-dependent tRNA halves enhance cell proliferation in breast and prostate cancers. [PDF]
, 2015 Sex hormones and their receptors play critical roles in the development and progression of the breast and prostate cancers. Here we report that a novel type of transfer RNA (tRNA)-derived small RNA, termed Sex HOrmone-dependent TRNA-derived RNAs (SHOT ...
Honda, Shozo +7 more
core +2 more sources
The human RBPome: From genes and proteins to human disease [PDF]
, 2015 RNA binding proteins (RBPs) play a central role in mediating post transcriptional regulation of genes. However less is understood about them and their regulatory mechanisms.
Hashemikhabir, Seyedsasan +2 more
core +1 more source