Alternative Spliceosome Assembly Pathways Revealed by Single-Molecule Fluorescence Microscopy
Cell Reports, 2013 Removal of introns from nascent transcripts (pre-mRNAs) by the spliceosome is an essential step in eukaryotic gene expression. Previous studies have suggested that the earliest steps in spliceosome assembly in yeast are highly ordered and the stable ...
Inna Shcherbakova +7 more
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Differential distribution of factors involved in pre-mRNA processing in the yeast cell nucleus [PDF]
, 1990 The yeast cell nucleus has previously been shown to be divided into two regions by a variety of microscopic approaches. We used antibodies specific for the 2,2,7-trimethylguanosine cap structure of small nuclear ribonucleic acids (snRNAs) and for a ...
Potashkin, J. A. +2 more
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Nucleic Acids Research, 1989 As demonstrated by RNase T1 protection assays at 0 degrees C without ATP, U1 and U5 snRNPs purified by isopycnic centrifugation in cesium chloride bind to the 5' and 3' splice sites of human beta-globin pre-mRNA, respectively. We also devised a saturation-complementation assay and have found that this purified U5 snRNP, unlike U1, successfully competes
J, Tazi +7 more
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Affinity purification of spliceosomes reveals that the precursor RNA processing protein PRP8, a protein in the U5 small nuclear ribonucleoprotein particle, is a component of yeast spliceosomes. [PDF]
Proceedings of the National Academy of Sciences, 1990 Nuclear pre-mRNA splicing in Saccharomyces cerevisiae, as in higher eukaryotes, occurs in large RNA-protein complexes called spliceosomes. The small nuclear RNA components, U1, U2, U4, U5, and U6, have been extensively studied; however, very little is known about the protein components of yeast spliceosomes. Here we use antibodies against the precursor
E, Whittaker, M, Lossky, J D, Beggs
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Friend of Prmt1, FOP is a novel component of the nuclear SMN complex isolated using biotin affinity purification [PDF]
, 2014 SMN (survival motor neuron protein) complexes are essential for the biogenesis of uridine-rich small nuclear ribonucleoproteins (UsnRNPs). During the biogenesis, the SMN complexes bound to UsnRNPs are transported from the cytoplasm to the nucleus, and ...
Ishikawa, H. (Hiroki) +11 more
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eLife, 2018 Transcription-translation feedback loops that comprise eukaryotic circadian clocks rely upon temporal delays that separate the phase of active transcription of clock genes, such as Drosophila period (per) and timeless (tim), from negative feedback by the
Iryna Shakhmantsir +3 more
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A subset of nuclear receptor coregulators act as coupling proteins during synthesis and maturation of RNA transcripts [PDF]
, 2005 Gene expression is a multistep process starting in the cell nucleus with the synthesis of the primary transcripts that undergo several modifications (including capping, splicing, and polyadenylation) leading to the export of the mature mRNAs into the ...
Auboeuf, Didier +5 more
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Splicing of the U6 RNA precursor is impaired in fission yeast pre-mRNA splicing mutants [PDF]
, 1989 U6 RNA is a member of a class of small abundant stable nuclear RNAs that are essential for splicing. In all species examined so far, the U6 RNA is a RNA polymerase III transcript.
Potashkin, J., Frendewey, D.
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The spliceosome, a potential Achilles heel of MYC-driven tumors [PDF]
, 2015 Alterations in RNA splicing are frequent in human tumors. Two recent studies of lymphoma and breast cancer have identified components of the spliceosome - the core splicing machinery - that are essential for malignant transformation driven by the ...
Anczukow, O., Krainer, A. R.
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Purification and characterization of pre-mRNA splicing factor SF2 from HeLa cells [PDF]
, 1990 SF2, an activity necessary for 5' splice site cleavage and lariat formation during pre-mRNA splicing in vitro, has been purified to near homogeneity from HeLa cells. The purest fraction contains only two related polypeptides of 33 kD.
Conway, G. C. +2 more
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