The Journal of biological chemistry, 2001 We describe a novel approach to identify RNA-protein cross-linking sites within native small nuclear ribonucleoprotein (snRNP) particles from HeLa cells. It combines immunoprecipitation of the UV-irradiated particles under semi-denaturing conditions with primer extension analysis of the cross-linked RNA moiety.
H, Urlaub +4 more
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A Role for Pre-mRNA-PROCESSING PROTEIN 40C in the Control of Growth, Development, and Stress Tolerance in Arabidopsis thaliana [PDF]
, 2019 Because of their sessile nature, plants have adopted varied strategies for growing and reproducing in an ever-changing environment. Control of mRNA levels and pre-mRNA alternative splicing are key regulatory layers that contribute to adjust and ...
Careno, Daniel Alejandro +6 more
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Genome-wide identification of genes involved in growth and fermentation activity at low temperature in Saccharomyces cerevisiae [PDF]
, 2016 Fermentation at low temperatures is one of the most popular current winemaking practices because of its reported positive impact on the aromatic profile of wines.
García Ríos, Estéfani +7 more
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Mud2 functions in transcription by recruiting the Prp19 and TREX complexes to transcribed genes [PDF]
, 2007 The different steps of gene expression are intimately linked to coordinate and regulate this complex process. During transcription, numerous RNA-binding proteins are already loaded onto the nascent mRNA and package the mRNA into a messenger ...
Andersen, Ulrik L. +8 more
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Selection of alternative 5 ' splice sites: Role of U1 snRNP and models for the antagonistic effects of SF2/ASF and hnRNP A1 [PDF]
, 2000 The first component known to recognize and discriminate among potential 5' splice sites (5'SSs) in pre-mRNA is the U1 snRNP. However, the relative levels of U1 snRNP binding to alternative 5'SSs do not necessarily determine the splicing outcome ...
Eperon, I. C. +6 more
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SmD3 regulates intronic noncoding RNA biogenesis [PDF]
, 2012 Accumulation of excess lipid in nonadipose tissues is associated with oxidative stress and organ dysfunction and plays an important role in diabetic complications.
Michel, Carlos I +3 more
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WRAP53 Unwrapped; Roles in Nuclear Architecture and Cancer [PDF]
, 2011 WRAP53 is a gene of multiple functions; it encodes for a natural antisense transcript that regulates the actions of the tumour suppressor p53, and it gives rise to a protein with oncogenic properties that is important for Cajal body formation and ...
Mahmoudi, Salah
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RNA (New York, N.Y.), 2001 We have purified the yeast U5 and U6 pre-mRNA splicing small nuclear ribonucleoproteins (snRNPs) by affinity chromatography and analyzed the associated polypeptides by mass spectrometry. The yeast U5 snRNP is composed of the two variants of U5 snRNA, six U5-specific proteins and the 7 proteins of the canonical Sm core.
Stevens, Scott W. +6 more
openaire +2 more sources
Proteomic analysis of interchromatin granule clusters [PDF]
, 2004 A variety of proteins involved in gene expression have been localized within mammalian cell nuclei in a speckled distribution that predominantly corresponds to interchromatin granule clusters (IGCs).
Saitoh, N. +5 more
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The special Sm core structure of the U7 snRNP: far-reaching significance of a small nuclear ribonucleoprotein [PDF]
, 2018 .: The polypeptide composition of the U7 small nuclear ribonucleoprotein (snRNP) involved in histone messenger RNA (mRNA) 3′ end formation has recently been elucidated.
Pillai, R., Schümperli, D.
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