A Beautiful Bind: Phage Display and the Search for Cell-Selective Peptides [PDF]
VirusesPhage display has advanced the discovery of peptides that selectively bind to a wide variety of cell surface molecules, offering new modalities to modulate disease-related protein–protein interactions (PPIs).
Babak Bakhshinejad, Saeedeh Ghiasvand
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The Use of Heterologous Antigens for Biopanning Enables the Selection of Broadly Neutralizing Nanobodies Against SARS-CoV-2 [PDF]
AntibodiesBackground: Since the emergence of SARS-CoV-2 in the human population, the virus genome has undergone numerous mutations, enabling it to enhance transmissibility and evade acquired immunity.
Vazirbek S. Aripov +14 more
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On the origin of non-specific binders isolated in the selection of phage display peptide libraries [PDF]
Frontiers in MicrobiologyOver the recent decades, phage display has been used successfully to identify a variety of peptides with diagnostic and therapeutic applications. Despite the significant role of this technology in the pharmaceutical industry, the affinity selection of ...
Babak Bakhshinejad +3 more
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Alpseq: an open-source workflow to turbocharge nanobody discovery with high-throughput sequencing [PDF]
mAbsNanobodies have emerged as promising tools for many biotechnological applications due to their small size, high stability and remarkable binding specificity.
Kathleen Zeglinski +9 more
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Preparation of Phage Display cDNA Libraries for Identifying Immunogenic Tumor Antigens: Challenges in Functional cDNA Presentation and Approaches to Overcoming Them [PDF]
VirusesCancer continues to represent a substantial burden in terms of its morbidity and mortality, underscoring the imperative for the development of novel and efficacious treatment modalities.
Nuša Brišar +2 more
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Using NGS to Uncover the Corruption of a Peptide Phage Display Selection [PDF]
Current Issues in Molecular BiologyPhage display has been widely used to identify peptides binding to a variety of biological targets. In the current work, we planned to select novel peptides targeting CD4 through screening of a commercial phage display library (New England Biolabs Ph.D ...
Danna Kamstrup Sell +7 more
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Protocol for screening and validating antibodies specific to protein phosphorylation sites using a set of yeast biopanning approaches [PDF]
STAR ProtocolsSummary: Developing antibodies with high specificity against post-translationally modified epitopes remains a challenge. Yeast biopanning is well suited in screening for high-specificity binders.
Monika Arbaciauskaite +4 more
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Chemosensors, 2021 Peptides can recognize and selectively bind to a wide variety of materials dependent on both their surface properties and the environment. Biopanning with phage or cell peptide display libraries can identify material-specific binding peptides.
Rachel Krabacher +5 more
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Phage display-derived binders able to distinguish Listeria monocytogenes from other Listeria species. [PDF]
PLoS ONE, 2013 The objective of this study was to produce phage display-derived binders with the ability to distinguish Listeria monocytogenes from other Listeria spp., which may have potential utility to enhance detection of Listeria monocytogenes.
Josephine Morton +5 more
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Phage-Display Based Discovery and Characterization of Peptide Ligands against WDR5
Molecules, 2021 WD40 is a ubiquitous domain presented in at least 361 human proteins and acts as scaffold to form protein complexes. Among them, WDR5 protein is an important mediator in several protein complexes to exert its functions in histone modification and ...
Jiawen Cao +10 more
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