Results 41 to 50 of about 5,817 (208)

Identification of Functional mimotopes of human Vasorin Ectodomain by Biopanning [PDF]

International Journal of Biological Sciences, 2018
Human vasorin (VASN) as a type I transmembrane protein, is a potential biomarker of hepatocellular carcinoma, which could expedite HepG2 cell proliferation and migration significantly in vitro. The ectodomain of VASN was proteolytically released to generate soluble VASN (sVASN), which was validated to be the active form.
Li, Da, Zhang, Tan, Yang, Xiqin, Geng, Jie, Li, Shaohua, Ding, Hongmei, Li, Hui, Huang, Aixue, Wang, Chaonan, Sun, Leqiao, Bai, Chenjun, Zhang, Heqiu, Li, Jie, Dong, Jie, Shao, Ningsheng   +14 more
openaire   +2 more sources

Mimotopes selected by biopanning with high-titer HIV-neutralizing antibodies in plasma from Chinese slow progressors

Brazilian Journal of Infectious Diseases, 2012
OBJECTIVE: One approach to identifying HIV-1 vaccine candidates is to dissect the natural antiviral immune response in treatment-naïve individuals infected for over ten years, considered slow progressor patients (SPs).
Xiaoli Zhang, Xiaoxu Han, Di Dai, Bao Mingjia, Zining Zhang, Min Zhang, Tristan Bice, Min Zhao, Yaming Cao, Hong Shang   +9 more
doaj   +3 more sources

Introduction of plasmid encoding for rare tRNAs reduces amplification bias in phage display biopanning

BioTechniques, 2015
Amplification bias is a major hurdle in phage display protocols because it imparts additional, unintended selection pressure beyond binding to the desired target.
Benjamin J. Umlauf, Michael J. McGuire, Kathlynn C. Brown   +2 more
doaj   +1 more source

Strategies to Screen Anti-AQP4 Antibodies from Yeast Surface Display Libraries

Antibodies, 2022
A rapid and effective method to identify disease-specific antibodies from clinical patients is important for understanding autoimmune diseases and for the development of effective disease therapies.
Aric Huang, Wei Jin, Ahmed S. Fahad, Brooklyn K. Mussman, Grazia Paola Nicchia, Bharat Madan, Matheus Oliveira de Souza, J. Daniel Griffin, Jeffrey L. Bennett, Antonio Frigeri, Cory J. Berkland, Brandon J. DeKosky   +11 more
doaj   +1 more source

High-Throughput Sequencing Enhanced Phage Display Identifies Peptides That Bind Mycobacteria [PDF]

, 2013
Bacterial cell wall components have been previously used as infection biomarkers detectable by antibodies. However, it is possible that the surface of the Mycobacterium tuberculosis (M.
Gresh, Lionel, Ioerger, Thomas R., Khati, Makobetsa, Ngubane, Nqobile A. C., Pym, Alexander, Rubin, Eric J., Sacchettini, James C., Willson, Richard C., Zhang, Yanjia J.   +8 more
core   +9 more sources

Biopanning of Antibody-Phage Clones Using Immunoplates Coated with Gel Slices of Electrophoresis: Immunogel-Biopanning

Biological and Pharmaceutical Bulletin, 2007
Biopanning of a phage library using a Western blotting membrane is difficult because of high background binding. We propose a reliable biopanning method, namely, immunogel-biopanning, which is performed using immunoplates coated with a molecular species fractionated from a crude sample by native polyacrylamide gel electrophoresis (PAGE).
Takayuki, Hamasaki, Shunsuke, Uchida, Tomoki, Yoshihara, Shuhei, Hashiguchi, Yuji, Ito, Kazuhisa, Sugimura   +5 more
openaire   +3 more sources

3in1: an ultra-fast phage display biopanning method overcoming the biopanning bias and yielding more diverse binders

BioTechniques
Phage display combined with biopanning is a powerful method for the discovery of therapeutic antibodies. Classic biopanning is lengthy and labor-intensive, requiring at least five days of work.
Samson Lichtenstein, Morgane Valles, Michael Mullin, Aleardo Morelli   +3 more
doaj   +1 more source

Development of anti-infectives using phage display: biological agents against bacteria, viruses, and parasites [PDF]

, 2012
The vast majority of anti-infective therapeutics on the market or in development are small molecules; however, there is now a nascent pipeline of biological agents in development.
Bishop-Hurley, Sharon L., Cooper, Matthew A., Huang, Johnny X.   +2 more
core   +1 more source

GPCR-Based Bioactive Peptide Screening Using Phage-Displayed Peptides and an Insect Cell System for Insecticide Discovery

Biomolecules, 2021
The discovery of new insecticides improves integrated pest management (IPM), but is usually a long high-risk process with a low probability of success.
Man-Yeon Choi, Robert K. Vander Meer
doaj   +1 more source

Theranostic applications of phage display to control leishmaniasis: Selection of biomarkers for serodiagnostics, vaccination, and immunotherapy [PDF]

, 2017
© 2015, Sociedade Brasileira de Medicina Tropical. All rights reserved. Phage display is a high-throughput subtractive proteomic technology used for the generation and screening of large peptide and antibody libraries.
Chávez-Fumagalli, Miguel A., Coelho, Eduardo A. F., Costa, Lourena E., Goulart, Luiz R., Soto, Manuel, Tavares, Carlos A. P.   +5 more
core   +1 more source