Affinity Capture Mass Spectrometry of Biomarker Proteins Using Peptide Ligands from Biopanning [PDF]
Affinity capture mass spectrometry was used to isolate and ionize protein A from Staphylococcus aureus from both a commercial source and cell culture lysate using matrix assisted laser desorption/ionization (MALDI) mass spectrometry. Two surfaces are compared: gold surfaces with immunoglobulin G covalently immobilized and silica surfaces with a ...
Erin M, Johnson +3 more
openaire +2 more sources
Preparation of hexagonal GeO2 particles with particle size and crystallinity controlled by peptides, silk and silk-peptide chimeras [PDF]
We demonstrate the use of silk based proteins to control the particle/crystallite size during GeO2 formation, using a bio-mimetic approach at circumneutral pH and ambient temperature. Multicrystalline GeO2 was prepared from germanium tetraethoxide (TEOG)
Avanzato +47 more
core +1 more source
Rapid, multiplexed microfluidic phage display [PDF]
The development of a method for high-throughput, automated proteomic screening could impact areas ranging from fundamental molecular interactions to the discovery of novel disease markers and therapeutic targets.
Ahmad, Habib +6 more
core
Subtractive phage display selection from canine visceral leishmaniasis identifies novel epitopes that mimic leishmania infantum antigens with potential serodiagnosis applications [PDF]
Visceral leishmaniasis (VL) is a zoonotic disease that is endemic to Brazil, where dogs are the main domestic parasite reservoirs, and the percentages of infected dogs living in regions where canine VL (CVL) is endemic have ranged from 10% to 62 ...
Andrade, Pedro H R +15 more
core +2 more sources
Astrochemistry on the EXPOSE/ISS and BIOPAN/Foton experiments [PDF]
AbstractWe describe three space experiments designed to expose to space conditions, and more specifically to solar UV radiation, selected samples of organic and mineral material.
Cottin, H. +14 more
openaire +2 more sources
We report an electrochemical biosensor that employs biopanning‐derived affinity peptides as selective receptors on a multifunctional chitosan–zwitterionic–gold nanoparticle (CS–ZI–AuNPs) nanohydrogel interface. The CS–ZI–AuNPs nanohydrogel exhibits antifouling properties, abundant sites for stable peptide immobilization, and efficient electrochemical ...
Hyo Jeong Yang +6 more
wiley +1 more source
Zearalenone (ZEN), a toxic estrogenic mycotoxin in cereals, threatens human and animal health through reproductive, immune, and cytotoxic effects, necessitating sensitive detection methods.
Ying Zeng +9 more
doaj +1 more source
Progress on Phage Display Technology: Tailoring Antibodies for Cancer Immunotherapy
The search for innovative anti-cancer drugs remains a challenge. Over the past three decades, antibodies have emerged as an essential asset in successful cancer therapy.
Renato Kaylan Alves França +7 more
doaj +1 more source
Transmissible gastroenteritis virus: Identification of M protein-binding peptide ligands with antiviral and diagnostic potential [PDF]
The membrane (M) protein is one of the major structural proteins of coronavirus particles. In this study, the M protein of transmissible gastroenteritis virus (TGEV) was used to biopan a 12-mer phage display random peptide library.
Ren, Xiaofeng +4 more
core +1 more source
Nectin‐4 is frequently overexpressed in solid tumors, yet many PDT photosensitizers show poor water solubility and nonspecific uptake. A Nectin‐4‐targeted nanobody–chlorin e6 conjugate (NPC) improves aqueous solubility and selectively accumulates in Nectin‐4‐positive tumor cells.
Haodian Wan +10 more
wiley +1 more source

