Results 21 to 30 of about 4,909 (142)

A CNN based m5c RNA methylation predictor

Scientific Reports, 2023
AbstractPost-transcriptional modifications of RNA play a key role in performing a variety of biological processes, such as stability and immune tolerance, RNA splicing, protein translation and RNA degradation. One of these RNA modifications is m5c which participates in various cellular functions like RNA structural stability and translation efficiency,
Irum Aslam, Sajid Shah, Saima Jabeen, Mohammed ElAffendi, Asmaa A. Abdel Latif, Nuhman Ul Haq, Gauhar Ali   +6 more
openaire   +3 more sources

Protocol for profiling RNA m⁵C methylation at base resolution using m⁵C-TAC-seq. [PDF]

STAR Protoc
RNA 5-methylcytosine (m5C) is a widespread modification and plays a crucial role in gene expression regulation. Here, we present a protocol for transcriptome-wide m5C methylome profiling at base resolution using bisulfite-free m5C detection strategy enabled by ten-eleven translocation (TET)-assisted chemical labeling sequencing (m5C-TAC-seq). We detail
Zhang X, Lu L, Yi C, Li X.
europepmc   +4 more sources

5-Methylcytosine profiles in mouse transcriptomes suggest the randomness of m5C formation catalyzed by RNA methyltransferase

BMC Research Notes, 2022
Objective 5-Methylcytosine (m5C) is a type of chemical modification on the nucleotides and is widespread in both DNA and RNA. Although the DNA m5C has been extensively studied over the past years, the distribution and biological function of RNA m5C still
Junfeng Liu
doaj   +1 more source

Overview of distinct 5-methylcytosine profiles of messenger RNA in normal and knock-down NSUN2 colorectal cancer cells

Frontiers in Genetics, 2023
Background: Colorectal cancer (CRC) is a harmful cancer with high morbidity and poor prognosis. There is growing evidence that RNA methylation is closely related to the occurrence of cancer and its malignant biological behavior.
Yu Lin, Yu Lin, Yu Lin, Zhifang Zhao, Wenqiang Nie, Manting Huang, Jiazhong Cai, Yadong Wang, Hesong Wang, Yongmei Huang, Yang Bai, Yang Bai   +11 more
doaj   +1 more source

NOP2-Mediated m5C Methylation Modification of LMNB2 mRNA Facilitates Colorectal Cancer Progression. [PDF]

Cancer Med
ABSTRACTBackgroundColorectal cancer (CRC) is a leading cause of cancer‐related mortality globally, yet current therapies exhibit suboptimal efficacy with limited prognostic improvement. RNA 5‐methylcytosine (m5C), a posttranscriptional modification, has been implicated in tumorigenesis and progression across malignancies. In our previous study, the m5C
Bi J, Huang Y, Hu W, Liu Y.
europepmc   +4 more sources

NSUN2 modified by SUMO-2/3 promotes gastric cancer progression and regulates mRNA m5C methylation. [PDF]

Cell Death Dis, 2021
AbstractThe 5-methylcytosine (m5C) RNA methyltransferase NSUN2 is involved in the regulation of cell proliferation and metastasis formation and is upregulated in multiple cancers. However, the biological significance of NSUN2 in gastric cancer (GC) and the modification of NSUN2 itself have not been fully investigated.
Hu Y, Chen C, Tong X, Chen S, Hu X, Pan B, Sun X, Chen Z, Shi X, Hu Y, Shen X, Xue X, Lu M.   +12 more
europepmc   +5 more sources

Folate regulates RNA m5C modification and translation in neural stem cells

BMC Biology, 2022
Background Folate is an essential B-group vitamin and a key methyl donor with important biological functions including DNA methylation regulation. Normal neurodevelopment and physiology are sensitive to the cellular folate levels.
Xiguang Xu, Zachary Johnson, Amanda Wang, Rachel L. Padget, James W. Smyth, Hehuang Xie   +5 more
doaj   +1 more source

Comprehensive Analysis of m5C RNA Methylation Regulator Genes in Clear Cell Renal Cell Carcinoma

International Journal of Genomics, 2021
Background. Recent research found that N5-methylcytosine (m5C) was involved in the development and occurrence of numerous cancers. However, the function and mechanism of m5C RNA methylation regulators in clear cell renal cell carcinoma (ccRCC) remains ...
Jiajin Wu, Chao Hou, Yuhao Wang, Zhongyuan Wang, Pu Li, Zengjun Wang   +5 more
doaj   +1 more source

NSUN2-Mediated m5C Methylation and METTL3/METTL14-Mediated m6A Methylation Cooperatively Enhance p21 Translation [PDF]

Journal of Cellular Biochemistry, 2017
N6-methyladenosine (m6A) and m5C methylation are two major types of RNA methylation, but the impact of joint modifications on the same mRNA is unknown. Here, we show that in p21 3'UTR, NSUN2 catalyzes m5C modification and METTL3/METTL14 catalyzes m6A modification. Interestingly, methylation at m6A by METTL3/METTL14 facilitates the methylation of m5C by
Qiu Li, Xiu Li, Hao Tang, Bin Jiang, Yali Dou, Myriam Gorospe, Wengong Wang   +6 more
openaire   +4 more sources

m5C RNA Methylation Primarily Affects the ErbB and PI3K–Akt Signaling Pathways in Gastrointestinal Cancer

Frontiers in Molecular Biosciences, 2020
5-Methylcytosine (m5C) is a kind of methylation modification that occurs in both DNA and RNA and is present in the highly abundant tRNA and rRNA. It has an important impact on various human diseases including cancer.
Shixin Xiang, Shixin Xiang, Yongshun Ma, Yongshun Ma, Jing Shen, Jing Shen, Yueshui Zhao, Yueshui Zhao, Xu Wu, Xu Wu, Mingxing Li, Mingxing Li, Xiao Yang, Parham Jabbarzadeh Kaboli, Parham Jabbarzadeh Kaboli, Fukuan Du, Fukuan Du, Huijiao Ji, Huijiao Ji, Yuan Zheng, Xiang Li, Jing Li, Qinglian Wen, Zhangang Xiao, Zhangang Xiao   +24 more
doaj   +1 more source