Results 41 to 50 of about 2,316 (143)

Methylation by NSun2 Represses the Levels and Function of MicroRNA 125b [PDF]

Molecular and Cellular Biology, 2014
Methylation is a prevalent posttranscriptional modification of RNAs. However, whether mammalian microRNAs are methylated is unknown. Here, we show that the tRNA methyltransferase NSun2 methylates primary (pri-miR-125b), precursor (pre-miR-125b), and mature microRNA 125b (miR-125b) in vitro and in vivo.
Shuai, Yuan, Hao, Tang, Junyue, Xing, Xiuqin, Fan, Xiaoyu, Cai, Qiu, Li, Pei, Han, Yuhong, Luo, Zhuojun, Zhang, Bin, Jiang, Yali, Dou, Myriam, Gorospe, Wengong, Wang   +12 more
openaire   +2 more sources

NSUN2-mediated m5C methylation of IRF3 mRNA negatively regulates type I interferon responses during various viral infections

Emerging Microbes and Infections, 2023
5-Methylcytosine (m5C) is a widespread post-transcriptional RNA modification and is reported to be involved in manifold cellular responses and biological processes through regulating RNA metabolism.
Hongyun Wang, Jiangpeng Feng, Cong Zeng, Jiejie Liu, Zhiying Fu, Dehe Wang, Yafen Wang, Lu Zhang, Jiali Li, Ao Jiang, Miao He, Yuanyuan Cao, Kun Yan, Hao Tang, Deyin Guo, Ke Xu, Xiang Zhou, Li Zhou, Ke Lan, Yu Zhou, Yu Chen   +20 more
doaj   +1 more source

NSun2 Promotes Cell Growth via Elevating Cyclin-Dependent Kinase 1 Translation [PDF]

Molecular and Cellular Biology, 2015
The tRNA methytransferase NSun2 promotes cell proliferation, but the molecular mechanism has not been elucidated. Here, we report that NSun2 regulates cyclin-dependent kinase 1 (CDK1) expression in a cell cycle-dependent manner. Knockdown of NSun2 decreased the CDK1 protein level, while overexpression of NSun2 elevated it without altering CDK1 mRNA ...
Junyue, Xing, Jie, Yi, Xiaoyu, Cai, Hao, Tang, Zhenyun, Liu, Xiaotian, Zhang, Jennifer L, Martindale, Xiaoling, Yang, Bin, Jiang, Myriam, Gorospe, Wengong, Wang   +10 more
openaire   +2 more sources

The RNA m⁵C Methylase NSUN2 Modulates Corneal Epithelial Wound Healing

Investigative Opthalmology & Visual Science, 2023
The emerging epitranscriptomics offers insights into the physiopathological roles of various RNA modifications. The RNA methylase NOP2/Sun domain family member 2 (NSUN2) catalyzes 5-methylcytosine (m5C) modification of mRNAs. However, the role of NSUN2 in corneal epithelial wound healing (CEWH) remains unknown.
Luo, Guangying, Xu, Weiwei, Chen, Xiaoyan, Xu, Wenji, Yang, Shuai, Wang, Jiao, Lin, Yong, Reinach, Peter S., Yan, Dongsheng   +8 more
openaire   +2 more sources

The m5C methyltransferase NSUN2 promotes codon‐dependent oncogenic translation by stabilising tRNA in anaplastic thyroid cancer

Clinical and Translational Medicine, 2023
Background Translation dysregulation plays a crucial role in tumourigenesis and cancer progression. Oncogenic translation relies on the stability and availability of tRNAs for protein synthesis, making them potential targets for cancer therapy.
Peng Li, Wenlong Wang, Ruixin Zhou, Ying Ding, Xinying Li   +4 more
doaj   +1 more source

NIPAL1 Drives a Metabolic‐Epigenetic Feedback Loop to Promote Lactate‐Mediated Immune Evasion in Esophageal Cancer

Advanced Science, EarlyView.
The study identifies a NIPAL1‐driven metabolic‐epigenetic circuit in esophageal squamous cell carcinoma that promotes glycolysis, lactate accumulation, and H3K18 histone lactylation, forming a self‐sustaining loop that suppresses CD8+ T cell immunity.
Ri‐Xin Chen, Xiao‐Dan Ma, Shui‐Dan Xu, Ao‐Tian Mo, Min‐Hua Deng, Jun‐Han Wu, Wei‐Tao Zhuang, Jin‐Ling Duan, Shu‐Huan Xie, Jin‐Long Lin, Peng Lin, Ji‐Ming Tang, Hai‐Yu Zhou, Xiao‐Song Ben, Gui‐Bin Qiao, Dan Xie   +15 more
wiley   +1 more source

Bridging maternal effects and epitranscriptomics: A novel perspective in developmental biology

Developmental Dynamics, EarlyView.
Abstract Maternal effects, encompassing both genetic (maternally expressed gene products) and non‐genetic (maternal state) influences, are powerful determinants of offspring phenotype, yet their RNA‐level mechanisms remain incompletely resolved. In parallel, epitranscriptomics, an emerging field centered on chemical modifications to RNA, has revealed ...
Ehsan Pashay Ahi
wiley   +1 more source

NSUN2-FOSB reciprocity facilitates leukemogenesis in an m5C-dependent manner by increasing BCL2L1 expression

Haematologica
NOP2/Sun RNA methyltransferase family member 2 (NSUN2) catalyzes 5-methylcytosine (m5C) modifications on RNA to regulate mRNA stability. However, its roles in normal hematopoiesis and leukemogenesis remain poorly understood.
Bin Zhou, Yigang Yuan, Yue Cai, Jingying Zhou, Liuzhi Shi, Yaqian Qin, Chen Meng, Shixin Zhang, Shirui Yu, Xinyao Chen, Xiaofei He, Shanshan Wu, Min Li, Xuanyu Yu, Yifen Shi, Chongyun Xing, Chiqi Chen, Meng Zhao, Shenmeng Gao   +18 more
doaj   +1 more source

GPRC5B Identified by m5C meRIP‐Seq Suppresses Apoptosis in Osteosarcoma Through NSUN2‐Mediated RNA Methylation

Cancer Science, EarlyView.
We performed the first transcriptome‐wide profiling of m5C modifications in osteosarcoma, analyzing four pairs of tumor and adjacent normal tissues. We revealed that NSUN2 suppresses apoptosis in osteosarcoma cells by promoting GPRC5B m5C modification and expression.
Zhenyi Chen, Min Yang, Xiaoxiao Liang, Zhiqiang Yang, Wulan Mai, Yuyang Wang, Yushen Lu, Ruimin Wang, Yuanhang Han, Yuanlong Xie, Lin Cai   +10 more
wiley   +1 more source

Impaired hippocampal NMDAR-LTP in a transgenic model of NSUN2-deficiency

Neurobiology of Disease, 2022
Biallelic loss-of-function NSUN2 mutations have recently been associated with cases of Autism Spectrum Condition (ASC), and NSun2-deficiency was also previously shown to cause a severe autosomal recessive intellectually disability disorder syndrome in which patients can sometimes display autistic behaviour.
George, Harry, Bashir, Zafar I., Hussain, Shobbir   +2 more
openaire   +5 more sources